Browsing by Author "Aedo, Geraldine"

Now showing 1 - 7 of 7
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    Doxorubicin induced senescence affects the integrity of the inner mitochondrial membrane in human iPS derived cardiomyocytes and primary rat cardiomyocytes
    (ELSEVIER, 2022) Morris, Silke; Molina-Riquelme, Isidora; Barrientos, Gonzalo; Bravo, Francisco; Aedo, Geraldine; Gomez, Wileidy; Psathaki, Katherina; Peischard, Stefan; Seebohm, Guiscard; Eisner, Veronica; Busch, Karin B.
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    Inner mitochondrial membrane structure and fusion dynamics are altered in senescent human iPSC-derived and primary rat cardiomyocytes
    (Elsevier B.V., 2023) Morris, Silke; Busch, Karin B.; Molina Riquelme, Isidora; Barrientos, Gonzalo; Bravo, Francisco; Aedo, Geraldine; Gómez, Wileidy; Lagos, Daniel; Eisner Sagues Veronica Raquel; Verdejo, Hugo; Peischard, Stefan; Seebohm, Guiscard; Psathaki, Olympia E.
    Dysfunction of the aging heart is a major cause of death in the human population. Amongst other tasks, mitochondria are pivotal to supply the working heart with ATP. The mitochondrial inner membrane (IMM) ultrastructure is tailored to meet these demands and to provide nano-compartments for specific tasks. Thus, function and morphology are closely coupled. Senescent cardiomyocytes from the mouse heart display alterations of the inner mitochondrial membrane. To study the relation between inner mitochondrial membrane architecture, dynamics and function is hardly possible in living organisms. Here, we present two cardiomyocyte senescence cell models that allow in cellular studies of mitochondrial performance. We show that doxorubicin treatment transforms human iPSC-derived cardiomyocytes and rat neonatal cardiomyocytes in an aged phenotype. The treated cardiomyocytes display double-strand breaks in the nDNA, have ?-galactosidase activity, possess enlarged nuclei, and show p21 upregulation. Most importantly, they also display a compromised inner mitochondrial structure. This prompted us to test whether the dynamics of the inner membrane was also altered. We found that the exchange of IMM components after organelle fusion was faster in doxorubicin-treated cells than in control cells, with no change in mitochondrial fusion dynamics at the meso-scale. Such altered IMM morphology and dynamics may have important implications for local OXPHOS protein organization, exchange of damaged components, and eventually the mitochondrial bioenergetics function of the aged cardiomyocyte.
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    Mitochondrial nucleoid dynamics perturbation by OPA1 disease-causing mutants
    (CELL PRESS, 2022) Eisner, Veronica; Macuada, Josefa; Vidal, Gonzalo; Aedo, Geraldine; Cartes-Saavedra, Benjamin; Rudge, Timothy
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    OPA1 disease-causing mutants perturb mitochondrial nucleoid cluster distribution
    (ELSEVIER, 2022) Eisner, Veronica; Macuada, Josefa; Vidal, Gonzalo; Molina-Riquelme, Isidora; Aedo, Geraldine; Lagos, Daniel; Perez, Nicolas; Rudge, Timothy; Cartes-Saavedra, Benjamin
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    Role of OPA1 ADOA-Causing Mutants in Mitochondrial Nucleoid Distribution
    (CELL PRESS, 2021) Macuada, Josefa; Aedo, Geraldine; Vidal, Gonzalo; Rudge, Timothy; Cartes Saavedra, Benjamin; Eisner, Veronica
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    Semi-Automated Method for Image Analysis of mtDNA Nucleoids Dynamics
    (CELL PRESS, 2021) Aedo, Geraldine; Macuada, Josefa; Cartes Saavedra, Benjamin; Vidal, Gonzalo; Rudge, Timothy; Eisner, Veronica
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    Zebrafish as an emerging model organism to study angiogenesis in development and regeneration
    (2016) Chávez, Myra; Aedo, Geraldine; Fierro, Fernando; Allende, Miguel; Egaña, José Tomás
    Angiogenesis is the process through which new blood vessels are formed from preexisting ones and plays a critical role in several conditions including embryonic development, tissue repair and disease. Moreover, enhanced therapeutic angiogenesis is a major goal in the field of regenerative medicine and efficient vascularization of artificial tissues and organs is one of the main hindrances in the implementation of tissue engineering approaches, while, on the other hand, inhibition of angiogenesis is a key therapeutic target to inhibit for instance tumor growth. During the last decades, the understanding of cellular and molecular mechanisms involved in this process has been matter of intense research. In this regard, several in vitro and in vivo models have been established to visualize and study migration of endothelial progenitor cells, formation of endothelial tubules and the generation of new vascular networks, while assessing the conditions and treatments that either promote or inhibit such processes. In this review, we address and compare the most commonly used experimental models to study angiogenesis in vitro and in vivo. In particular, we focus on the implementation of the zebrafish (Danio rerio) as a model to study angiogenesis and discuss the advantages and not yet explored possibilities of its use as model organism.