Browsing by Author "Aguayo, C."

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    Differential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells
    (W B SAUNDERS CO LTD, 2010) Guzman Gutierrez, E.; Sandoval, C.; Nova, E.; Castillo, J. L.; Vera, J. C.; Lamperti, L.; Krause, B.; Salomon, C.; Sepulveda, C.; Aguayo, C.; Sobrevia, L.
    Extracellular adenosine removal is via human equilibrative nucleoside transporters 1 (hENT1) and 2 (hENT2) in the endothelium, thus regulating adenosine-induced revascularization and angiogenesis. Since human endothelial progenitor cells (hEPCs) promote revascularization, we hypothesize differential expression of nucleoside transporters in hEPCs. hEPCs were cultured 3 (hEPC-3d) or 14 (hEPC-14d) days. RT-PCR for prominin 1, CD34, octamer-4, kinase insert domain receptor, oxidized low-density lipoprotein (lectin-like) receptor 1 and tyrosine endothelial kinase was used to evaluate phenotypic differentiation. Flow cytometry was used to estimate CD34(+)/KDR- (non-differentiated), CD34(-)/KDR+ (differentiated) or CD34(+)/KDR+ (mixed) cell populations. Adenosine transport was measured in absence or presence of sodium, S-(4-nitrobenzyl)-6-thio-inosine (NBTI, 1-10 mu M), inosine, hypoxanthine or guanine (0.1-5 mM), hENTs protein abundance by western blot, and hENTs, hCNT1, hCNT2 and hCNT3 mRNA expression by real time RT-PCR. hEPC-3d cells were CD34(+)/KDR- compared with hEPC-14d cells that were CD34(-)/KDR+ hEPC-3d cells exhibit hENT1-like adenosine transport (NBTI-sensitive, Na+-independent), which is absent in hEPC-14d cells. hEPC-14d cells exhibit two transport components: component 1 (NBTI insensitive, Na+-independent) and component 2 (NBTI insensitive, Na+-dependent, Hill coefficient similar to 1.8), the latter resembling CNT3-like transport. hEPC-3d cells express hENT1 protein and mRNA, which is reduced (similar to 90%) in hEPC-14d cells, but instead only hCNT3 mRNA is expressed in this cell type. hENT2, hCNT1 and hCNT2 were undetectable in hEPCs. Thus, hEPCs exhibit a differential expression of hENT1 and hCNT3 functional nucleoside transporters, which could be related with its differentiation stage. (c) 2010 Elsevier Ltd. All rights reserved.
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    Increased placental angiogenesis in late and early onset pre-eclampsia is associated with differential activation of vascular endothelial growth factor receptor 2
    (W B SAUNDERS CO LTD, 2014) Escudero, C.; Celis, C.; Saez, T.; San Martin, S.; Valenzuela, F. J.; Aguayo, C.; Bertoglia, P.; Roberts, J. M.; Acurio, J.
    Introduction: Placentas from both early-onset (EOPE) and late-onset pre-eclampsia (LOPE) exhibit signs of underperfusion, which in turn, may be associated with altered angiogenesis. Tyrosine 951 (Y951) and Y1175 phosphorylation of the vascular endothelial growth factor receptor 2 (VEGFR2) induced by VEGF triggers the angiogenesis process. Endothelial markers such as CD31 and CD34 have been used for estimating angiogenic processes in several tissues, including placenta. We asked whether vascular density in placental villi was related to Y951/Y1175 phosphorylation of VEGFR2 in LOPE or EOPE.
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    Insulin Reverses D-Glucose-Increased Nitric Oxide and Reactive Oxygen Species Generation in Human Umbilical Vein Endothelial Cells
    (2015) Gonzalez, M.; Rojas, S.; Avila. P.; Cabrera, L.; Villalobos Labra, Roberto Esteban; Palma, C.; Aguayo, C.; Pena, E.; Salsoso Rodríguez, M. Rocío; Leiva Mendoza, Andrea Alejandra; Sobrevía Luarte, Luis Alberto; Gallardo, V.; Guzman-Gutierrez, E.; Saez, T.; Sanhueza, C.; Pardo, F.