Browsing by Author "Aguayo Paul, Sebastián"
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- ItemA multi-scale biophysical approach to develop structure-property relationships in oral biofilms.(2018) Pattem, M.; Davrandi, M.; Aguayo Paul, Sebastián; Allan, E.; Spratt, D.; Bozec, Laurent.
- ItemA simple and robust method for pre-wetting poly (lactic-co-glycolic) acid microspheres.(2015) Wright, Bernice.; Parmar, Nina; Bozec, Laurent.; Aguayo Paul, Sebastián; Day, Richard M.
- ItemAssociation between alzheimer's disease and oral and gut microbiota : are pore forming proteins the missing link?(2018) Aguayo Paul, Sebastián; Schuh, Christina María Anna Pía; Vicente, Benjamín; Aguayo, Luis Gerardo
- ItemAtomic force microscopy-mediated mechanobiological profiling of complex human tissues(2023) Cho, David H.; Aguayo Paul, Sebastián; Cartagena-Rivera, Alexander X.Tissue mechanobiology is an emerging field with the overarching goal of understanding the interplay between biophysical and biochemical responses affecting development, physiology, and disease. Changes in mechanical properties including stiffness and viscosity have been shown to describe how cells and tissues respond to mechanical cues and modify critical biological functions. To quantitatively characterize the mechanical properties of tissues at physiologically relevant conditions, atomic force microscopy (AFM) has emerged as a highly versatile biomechanical technology. In this review, we describe the fundamental principles of AFM, typical AFM modalities used for tissue mechanics, and commonly used elastic and viscoelastic contact mechanics models to characterize complex human tissues. Furthermore, we discuss the application of AFM-based mechanobiology to characterize the mechanical responses within complex human tissues to track their developmental, physiological/functional, and diseased states, including oral, hearing, and cancer45 related tissues. Finally, we discuss the current outlook and challenges to further advance the field of tissue mechanobiology. Altogether, AFM-based tissue mechanobiology provides a mechanistic understanding of biological processes governing the unique functions of tissues
- ItemCase‐based learning to teach scientific thinking to dental students(2022) Aguayo Paul, Sebastián; Benso, Bruna; Cantarutti Martínez, Cynthia; Ortuño Borroto, Duniel; Véliz, Claudia P.
- ItemEarly adhesion of Candida albicans onto dental acrylic surfaces.(2017) Aguayo Paul, Sebastián; Marshall, H.; Pratten, J.; Bradshaw, D.; Brown, J.S.; Porter, S.R.; Spratt, D.; Bozec, Laurent.
- ItemExosome-like vesicles in Apis mellifera bee pollen, honey and royal jelly contribute to their antibacterial and pro-regenerative activity(2019) Schuh, Christina M. A. P.; Aguayo Paul, Sebastián; Zavala, Gabriela; Khoury, Maroun
- ItemExtracellular Vesicles derived from Apis mellifera Royal Jelly promote wound healing by modulating inflammation and cellular responses(2022) Álvarez, Simón; Contreras Kallens, Pamina; Aguayo Paul, Sebastián; Ramírez, Orlando; Vallejos, Catalina; Ruiz, Jorge; Carrasco Gallardo, Eva; Troncoso Vera, Stephanie; Morales, Bernardo; Schuh, ChristinaApis mellifera Royal Jelly (RJ) is a well-known remedy in traditional medicine around the world and its versatile effects range from antibacterial to anti-inflammatory properties and pro-regenerative properties. Several active compounds have been identified, however, the mechanisms of action still remain widely unknown. As a glandular product, RJ has been shown to contain a substantial number of extracellular vesicles (EVs) and in this study, we aimed to investigate the extent of involvement of RJEVs in wound healing associated effects. Molecular analysis of RJEVs verified the presence of important conserved exosomal markers such as CD63 and syntenin, as well as cargo molecules MRJP1, defensin-1 and jellein-3. RJEV internalization analysis demonstrated the involvement of membrane fusion as well as macropinocytosis or clathrin-dependent endocytosis into mammalian cells. Furthermore, RJEVs have demonstrated to modulate MSCs differentiation and secretome, as well as decrease LPS-induced inflammation in RAW 264.7 macrophages by blocking the MAPK pathway. In vivo studies confirmed anti-bacterial effects of RJEVs, and demonstrated an acceleration of wound healing in a splinted mouse model. Summarizing, this study suggests that RJEVs of potentially exosomal origin play a crucial role in the known effects of RJ by modulating the inflammatory phase and cellular response in wound healing.
- ItemGenerative artificial intelligence in dentistry: Current approaches and future challenges(2024) Villena, F.; Véliz, Claudia P.; García-Huidobro Munita, Rosario; Aguayo Paul, SebastiánArtificial intelligence (AI) has become a commodity for people because of the advent of generative AI (GenAI) models that bridge the usability gap of AI by providing a natural language interface to interact with complex models. These GenAI models range from text generation - such as two-way chat systems - to the generation of image or video from textual descriptions input by a user. These advancements in AI have impacted Dentistry in multiple aspects. In dental education, the student now has the opportunity to solve a plethora of questions by only prompting a GenAI model and have the answer in a matter of seconds. GenAI models can help us deliver better patient healthcare by helping practitioners gather knowledge quickly and efficiently. Finally, GenAI can also be used in dental research, where the applications range from new drug discovery to assistance in academic writing. In this review, we first define GenAI models and describe their multiple generation modalities; then, we explain and discuss their current and potential applications in Dentistry; and finally, we describe the challenges these new technologies impose in our area.
- ItemIn Vivo Relationship between the Nano-Biomechanical Properties of Streptococcal Polysaccharide Capsules and Virulence Phenotype(2020) Marshall, H; Aguayo Paul, Sebastián; Kilian, M; Petersen, F; Bozec, L; Brown, J
- ItemInfluence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells(2016) Aguayo Paul, Sebastián; Strange, Adam P.; Gadegaard, Nikolaj J.; Dalby, Matthew; Bozec, Laurent.
- ItemMicrofabrication-based engineering of biomimetic dentin-like constructs to simulate dental aging(2024) Álvarez Martínez, Simón Esteban; Morales, José; Tiozzo-Lyon, Paola; Berrios Segovia, Pablo Germán; Barraza Montenegro, Valentina Maríia; Simpson, Kevin; Ravasio, Andrea; Monforte Vila, Xavier; Teuschl-Woller, Andreas; Schuh, Christina MAP; Aguayo Paul, SebastiánHuman dentin is a highly organized dental tissue displaying a complex microarchitecture consisting of micrometer-sized tubules encased in a mineralized type-I collagen matrix. As such, it serves as an important substrate for the adhesion of microbial colonizers and oral biofilm formation in the context of dental caries disease, including root caries in the elderly. Despite this issue, there remains a current lack of effective biomimetic in vitro dentin models that facilitate the study of oral microbial adhesion by considering the surface architecture at the micro- and nanoscales. Therefore, the aim of this study was to develop a novel in vitro microfabricated biomimetic dentin surface that simulates the complex surface microarchitecture of exposed dentin. For this, a combination of soft lithography microfabrication and biomaterial science approaches were employed to construct a micropitted PDMS substrate functionalized with mineralized type-I collagen. These dentin analogs were subsequently glycated with methylglyoxal (MGO) to simulate dentin matrix aging in vitro and analyzed utilizing an interdisciplinary array of techniques including atomic force microscopy (AFM), elemental analysis, and electron microscopy. AFM force-mapping demonstrated that the nanomechanical properties of the biomimetic constructs were within the expected biological parameters, and that mineralization was mostly predominated by hydroxyapatite deposition. Finally, dual-species biofilms of Streptococcus mutans and Candida albicans were grown and characterized on the biofunctionalized PDMS microchips, demonstrating biofilm-specific morphologic characteristics and confirming the suitability of this model for the study of early biofilm formation under controlled conditions. Overall, we expect that this novel biomimetic dentin model could serve as an in vitro platform to study oral biofilm formation or dentin–biomaterial bonding in the laboratory without the need for animal or human tooth samples in the future.
- ItemModulatory Effect of Glycated Collagen on Oral Streptococcal Nanoadhesion(2020) Schuh, C. M. A. P.; Benso, Bruna; Naulin Gysling, Pamela Alejandra; Barrera Rojas, Nelson Patricio; Bozec, L.; Aguayo Paul, Sebastián
- ItemNanoadhesion of Staphylococcus aureus onto titanium implant surfaces(2015) Aguayo Paul, Sebastián; Donos, Nikolaos; Spratt, David Alexander; Bozec, Laurent.
- ItemNonmotile Single-Cell Migration as a Random Walk in Nonuniformity: The Extreme Dumping Limit for Cell-to-Cell Communications(2018) Panotopoulos, Grigorios P.; Aguayo Paul, Sebastián; Haidar, Ziyad S.
- ItemPotential Novel Strategies for the Treatment of Dental Pulp-Derived Pain: Pharmacological Approaches and Beyond(2019) Schuh, C. M. A. P.; Benso, Bruna; Aguayo Paul, Sebastián
- ItemProbing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy.(2016) Aguayo Paul, Sebastián; Donos, Nikolaos; Spratt D.; Bozec, Laurent.
- ItemQuantitative nanohistological investigation of scleroderma: An atomic force microscopy-based approach to disease characterization.(2017) Strange, Adam P.; Aguayo Paul, Sebastián; Ahmed, Tarek R.; Mordan, Nichola J.; Stratton, Richard J.; Porter, Stephen R.; Parekh, Susan; Bozec, Laurent.
- ItemRoyal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses(2023) Álvarez, Simón; Contreras-Kallen, Pamina; Aguayo Paul, Sebastián; Ramírez, Orlando; Vallejos, Catalina; Ruiz, Jorge; Carrasco-Gallardo, Eva; Troncoso-Vera, Stefanie; Morales, Bernardo; Schuh, Christina M.A.P.Apis mellifera royal jelly (RJ) is a well-known remedy in traditional medicine around the world and its versatile effects range from antibacterial to anti-inflammatory properties and pro-regenerative properties. As a glandular product, RJ has been shown to contain a substantial number of extracellular vesicles (EVs), and, in this study, we aimed to investigate the extent of involvement of RJEVs in wound healing-associated effects. Molecular analysis of RJEVs verified the presence of exosomal markers such as CD63 and syntenin, and cargo molecules MRJP1, defensin-1, and jellein-3. Furthermore, RJEVs were demonstrated to modulate mesenchymal stem cell (MSC) differentiation and secretome, as well as decrease LPS-induced inflammation in macrophages by blocking the mitogen-activated protein kinase (MAPK) pathway. In vivo studies confirmed antibacterial effects of RJEVs and demonstrated an acceleration of wound healing in a splinted mouse model. This study suggests that RJEVs play a crucial role in the known effects of RJ by modulating the inflammatory phase and cellular response in wound healing. Transfer of RJ into the clinics has been impeded by the high complexity of the raw material. Isolating EVs from the raw RJ decreases the complexity while allowing standardization and quality control, bringing a natural nano-therapy one step closer to the clinics.
- ItemSingle-bacterium nanomechanics in biomedicine : unravelling the dynamics of bacterial cells.(2015) Aguayo Paul, Sebastián; Donos, Nikolaos; Spratt D.; Bozec, Laurent.