Browsing by Author "Aguayo Paul, Sebastián"
Now showing 1 - 20 of 20
Results Per Page
Sort Options
- ItemA multi-scale biophysical approach to develop structure-property relationships in oral biofilms.(2018) Pattem, M.; Davrandi, M.; Aguayo Paul, Sebastián; Allan, E.; Spratt, D.; Bozec, Laurent.
- ItemA simple and robust method for pre-wetting poly (lactic-co-glycolic) acid microspheres.(2015) Wright, Bernice.; Parmar, Nina; Bozec, Laurent.; Aguayo Paul, Sebastián; Day, Richard M.
- ItemAssociation between alzheimer's disease and oral and gut microbiota : are pore forming proteins the missing link?(2018) Aguayo Paul, Sebastián; Schuh, Christina María Anna Pía; Vicente, Benjamín; Aguayo, Luis Gerardo
- ItemAtomic force microscopy-mediated mechanobiological profiling of complex human tissues(2023) Cho, David H.; Aguayo Paul, Sebastián; Cartagena-Rivera, Alexander X.Tissue mechanobiology is an emerging field with the overarching goal of understanding the interplay between biophysical and biochemical responses affecting development, physiology, and disease. Changes in mechanical properties including stiffness and viscosity have been shown to describe how cells and tissues respond to mechanical cues and modify critical biological functions. To quantitatively characterize the mechanical properties of tissues at physiologically relevant conditions, atomic force microscopy (AFM) has emerged as a highly versatile biomechanical technology. In this review, we describe the fundamental principles of AFM, typical AFM modalities used for tissue mechanics, and commonly used elastic and viscoelastic contact mechanics models to characterize complex human tissues. Furthermore, we discuss the application of AFM-based mechanobiology to characterize the mechanical responses within complex human tissues to track their developmental, physiological/functional, and diseased states, including oral, hearing, and cancer45 related tissues. Finally, we discuss the current outlook and challenges to further advance the field of tissue mechanobiology. Altogether, AFM-based tissue mechanobiology provides a mechanistic understanding of biological processes governing the unique functions of tissues
- ItemCase‐based learning to teach scientific thinking to dental students(2022) Aguayo Paul, Sebastián; Benso, Bruna; Cantarutti Martínez, Cynthia; Ortuño Borroto, Duniel; Véliz, Claudia P.
- ItemEarly adhesion of Candida albicans onto dental acrylic surfaces.(2017) Aguayo Paul, Sebastián; Marshall, H.; Pratten, J.; Bradshaw, D.; Brown, J.S.; Porter, S.R.; Spratt, D.; Bozec, Laurent.
- ItemExosome-like vesicles in Apis mellifera bee pollen, honey and royal jelly contribute to their antibacterial and pro-regenerative activity(2019) Schuh, Christina M. A. P.; Aguayo Paul, Sebastián; Zavala, Gabriela; Khoury, Maroun
- ItemExtracellular Vesicles derived from Apis mellifera Royal Jelly promote wound healing by modulating inflammation and cellular responses(2022) Álvarez, Simón; Contreras Kallens, Pamina; Aguayo Paul, Sebastián; Ramírez, Orlando; Vallejos, Catalina; Ruiz, Jorge; Carrasco Gallardo, Eva; Troncoso Vera, Stephanie; Morales, Bernardo; Schuh, ChristinaApis mellifera Royal Jelly (RJ) is a well-known remedy in traditional medicine around the world and its versatile effects range from antibacterial to anti-inflammatory properties and pro-regenerative properties. Several active compounds have been identified, however, the mechanisms of action still remain widely unknown. As a glandular product, RJ has been shown to contain a substantial number of extracellular vesicles (EVs) and in this study, we aimed to investigate the extent of involvement of RJEVs in wound healing associated effects. Molecular analysis of RJEVs verified the presence of important conserved exosomal markers such as CD63 and syntenin, as well as cargo molecules MRJP1, defensin-1 and jellein-3. RJEV internalization analysis demonstrated the involvement of membrane fusion as well as macropinocytosis or clathrin-dependent endocytosis into mammalian cells. Furthermore, RJEVs have demonstrated to modulate MSCs differentiation and secretome, as well as decrease LPS-induced inflammation in RAW 264.7 macrophages by blocking the MAPK pathway. In vivo studies confirmed anti-bacterial effects of RJEVs, and demonstrated an acceleration of wound healing in a splinted mouse model. Summarizing, this study suggests that RJEVs of potentially exosomal origin play a crucial role in the known effects of RJ by modulating the inflammatory phase and cellular response in wound healing.
- ItemIn Vivo Relationship between the Nano-Biomechanical Properties of Streptococcal Polysaccharide Capsules and Virulence Phenotype(2020) Marshall, H; Aguayo Paul, Sebastián; Kilian, M; Petersen, F; Bozec, L; Brown, J
- ItemInfluence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells(2016) Aguayo Paul, Sebastián; Strange, Adam P.; Gadegaard, Nikolaj J.; Dalby, Matthew; Bozec, Laurent.
- ItemModulatory Effect of Glycated Collagen on Oral Streptococcal Nanoadhesion(2020) Schuh, C. M. A. P.; Benso, Bruna; Naulin Gysling, Pamela Alejandra; Barrera Rojas, Nelson Patricio; Bozec, L.; Aguayo Paul, Sebastián
- ItemNanoadhesion of Staphylococcus aureus onto titanium implant surfaces(2015) Aguayo Paul, Sebastián; Donos, Nikolaos; Spratt, David Alexander; Bozec, Laurent.
- ItemNonmotile Single-Cell Migration as a Random Walk in Nonuniformity: The Extreme Dumping Limit for Cell-to-Cell Communications(2018) Panotopoulos, Grigorios P.; Aguayo Paul, Sebastián; Haidar, Ziyad S.
- ItemPotential Novel Strategies for the Treatment of Dental Pulp-Derived Pain: Pharmacological Approaches and Beyond(2019) Schuh, C. M. A. P.; Benso, Bruna; Aguayo Paul, Sebastián
- ItemProbing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy.(2016) Aguayo Paul, Sebastián; Donos, Nikolaos; Spratt D.; Bozec, Laurent.
- ItemQuantitative nanohistological investigation of scleroderma: An atomic force microscopy-based approach to disease characterization.(2017) Strange, Adam P.; Aguayo Paul, Sebastián; Ahmed, Tarek R.; Mordan, Nichola J.; Stratton, Richard J.; Porter, Stephen R.; Parekh, Susan; Bozec, Laurent.
- ItemRoyal jelly extracellular vesicles promote wound healing by modulating underlying cellular responses(2023) Álvarez, Simón; Contreras-Kallen, Pamina; Aguayo Paul, Sebastián; Ramírez, Orlando; Vallejos, Catalina; Ruiz, Jorge; Carrasco-Gallardo, Eva; Troncoso-Vera, Stefanie; Morales, Bernardo; Schuh, Christina M.A.P.Apis mellifera royal jelly (RJ) is a well-known remedy in traditional medicine around the world and its versatile effects range from antibacterial to anti-inflammatory properties and pro-regenerative properties. As a glandular product, RJ has been shown to contain a substantial number of extracellular vesicles (EVs), and, in this study, we aimed to investigate the extent of involvement of RJEVs in wound healing-associated effects. Molecular analysis of RJEVs verified the presence of exosomal markers such as CD63 and syntenin, and cargo molecules MRJP1, defensin-1, and jellein-3. Furthermore, RJEVs were demonstrated to modulate mesenchymal stem cell (MSC) differentiation and secretome, as well as decrease LPS-induced inflammation in macrophages by blocking the mitogen-activated protein kinase (MAPK) pathway. In vivo studies confirmed antibacterial effects of RJEVs and demonstrated an acceleration of wound healing in a splinted mouse model. This study suggests that RJEVs play a crucial role in the known effects of RJ by modulating the inflammatory phase and cellular response in wound healing. Transfer of RJ into the clinics has been impeded by the high complexity of the raw material. Isolating EVs from the raw RJ decreases the complexity while allowing standardization and quality control, bringing a natural nano-therapy one step closer to the clinics.
- ItemSingle-bacterium nanomechanics in biomedicine : unravelling the dynamics of bacterial cells.(2015) Aguayo Paul, Sebastián; Donos, Nikolaos; Spratt D.; Bozec, Laurent.
- ItemType I collagen hydrogels as a delivery matrix for royal jelly derived extracellular vesicles(2020) Ramirez, O. J.; Alvarez, S.; Contreras-Kallens, P.; Barrera Rojas, Nelson Patricio; Aguayo Paul, Sebastián; Schuh, C. M. A. P.
- ItemUltrastructural characterisation of young and aged dental enamel by atomic force microscopy(2022) Leiva Sabadini, Camila Andrea; Schuh, Christina MAP; Barrera Rojas, Nelson Patricio; Aguayo Paul, SebastiánRecent advances in atomic force microscopy (AFM) have allowed the characterisation of dental-associated biomaterials and biological surfaces with high-resolution and minimal sample preparation. In this context, the topography of dental enamel – the hardest mineralised tissue in the body – has been explored with AFM-based approaches at the micro-scale. With age, teeth are known to suffer changes that can impact their structural stability and function; however, changes in enamel structure because of ageing have not yet been explored with nanoscale resolution. Therefore, the aim of this exploratory work was to optimise an approach to characterise the ultrastructure of dental enamel and determine potential differences in topography, hydroxyapatite (HA) crystal size, and surface roughness at the nanoscale associated to ageing. For this, a total of six teeth were collected from human donors from which enamel specimens were prepared. By employing AC mode imaging, HA crystals were characterised in both transversal and longitudinal orientation with high-resolution in environmental conditions. Sound superficial enamel displayed the presence of a pellicle-like coating on its surface, that was not observable on cleaned specimens. Acidetching exposed crystals that were imaged and morphologically characterised in highresolution at the nanoscale in both the external and internal regions of enamel in older and younger specimens. Our results demonstrated important individual variations in HA crystal width and roughness parameters across the analysed specimens; however, an increase in surface roughness and decrease in HA width was observed for the pooled older external enamel group compared to younger specimens. Overall, high-resolution AFM was an effective approach for the qualitative and quantitative characterisation of human dental enamel ultrastructure at the nanometre range. Future work should focus on exploring the ageing of dental enamel with increased sample sizes to compensate for individual differences as well as other potential confounding factors such as behavioural habits and mechanical forces.