Browsing by Author "Aguilera, J"

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    Analgesia after thoracotomy: Epidural fentanyl/bupivacaine compared with intercostal nerve block plus intravenous morphine
    (W B SAUNDERS CO-ELSEVIER INC, 2004) Concha, M; Dagnino, J; Cariaga, M; Aguilera, J; Aparicio, R; Guerrero, M
    Objectives: Intercostal nerve blockade plus intravenous (IV) patient-controlled analgesia (PCA) could be an easier and safer alternative to epidural analgesia for postthoracotomy pain, but information about the efficacy of this technique is scarce. The objective of this randomized study was to compare the quality of analgesia and lung function in 2 groups of patients undergoing pulmonary surgery through a posterolateral thoracotomy.
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    PAF receptor and PAF acetylhydrolase expression in the endosalpinx of the human Fallopian tube: possible role of embryo-derived PAF in the control of embryo transport to the uterus
    (2001) Velasquez, LA; Maisey, K; Fernandez, R; Valdes, D; Cardenas, H; Imarai, M; Delgado, J; Aguilera, J
    BACKGROUND: Prostaglandin-E-2 and platelet-activating factor (PAF) are embryonic-derived signals that time embryo passage into the uterus in the mare and hamster respectively. PAF-like activity is detectable in the spent media of preimplantation human embryos and it has been suggested that PAF may be the embryonic signal that controls embryo transport to the uterus in our species. The actions of PAF are regulated at the level of its synthesis and degradation as well as the expression of a specific cell surface receptor (PAFr). The enzyme PAF acetylhydrolase (PAF-AH) degrades PAR This study was undertaken to examine whether or not PAFr and PAF-AH are expressed in the human Fallopian tube and to identify the cell types in which they are expressed. METHODS: The presence of PAFr mRNA in tissue extracts was investigated using reverse transcription-polymerase chain reaction. We amplified the predicted amplicon for PAFr mRNA from RNA samples extracted from Fallopian tubes. The expression of PAF-AH was detected by Western blot and the localization of PAFr and PAF-AH proteins was detected by immunohistochemistry. RESULTS: Utilizing antibodies against PAFr and PAF-AH, co-localization of the two proteins in the epithelium and stromal cells were demonstrated. CONCLUSIONS: These observations show that the human Fallopian tube expresses PAFr and PAF-AH at a location compatible with the proposed paracrine role of early embryo-derived PAF.
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    Smooth muscle activity in the oviduct is regulated by sexual hormones.
    (SOC STUDY REPRODUCTION, 1996) Villalon, M; Budinich, M; Hermoso, M; Aguilera, J; Saez, JC