Browsing by Author "Araos, Joaquin"
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- ItemAcute lung injury secondary to hydrochloric acid instillation induces small airway hyperresponsiveness(2021) Basoalto Escobar, Roque Ignacio; Damiani Rebolledo, Luis Felipe; Bachmann Barron, María Consuelo; Fonseca, Marcelo; Barros, Marisol; Soto Muñoz, Dagoberto Igor; Araos, Joaquin; Jalil Contreras, Yorschua Frederick; Dubo, Sebastian; Retamal, Jaime; Bugedo Tarraza, Guillermo Jaime; Henriquez, Mauricio; Bruhn Cruz, Alejandro RodrigoBackground: Acute respiratory distress syndrome (ARDS) is a severe form of respiratory failure characterized by altered lung mechanics and poor oxygenation. Bronchial hyperresponsiveness has been reported in ARDS survivors and animal models of acute lung injury. Whether this hyperreactivity occurs at the small airways or not is unknown. Objective: To determine ex-vivo small airway reactivity in a rat model of acute lung injury (ALI) by hydrochloric acid (HCl) instillation. Methods: Twelve anesthetized rats were connected to mechanical ventilation for 4-hour, and randomly allocated to either ALI group (HCl intratracheal instillation; n=6) or Sham (intratracheal instillation of 0.9% NaCl; n=6). Oxygenation was assessed by arterial blood gases. After euthanasia, tissue samples from the right lung were harvested for histologic analysis and wet-dry weight ratio assessment. Precision cut lung slice technique (100-200 pm diameter) was applied in the left lung to evaluate ex vivo small airway constriction in response to histamine and carbachol stimulation, using phase-contrast video microscopy. Results: Rats from the ALI group exhibited hypoxemia, worse histologic lung injury, and increased lung wet-dry weight ratio as compared with the sham group. The bronchoconstrictor responsiveness was significantly higher in the ALI group, both for carbachol (maximal contraction of 84.5 +/- 2.5% versus 61.4 +/- 4.2% in the Sham group, P<0.05), and for histamine (maximal contraction of 78.6 +/- 5.3% versus 49.6 +/- 5.3% in the Sham group, P<0.05). Conclusion: In an animal model of acute lung injury secondary to HCL instillation, small airway hyperresponsiveness to carbachol and histamine is present. These results may provide further insight into the pathophysiologi of ARDS.
- ItemDistribution and Magnitude of Regional Volumetric Lung Strain and Its Modification by PEEP in Healthy Anesthetized and Mechanically Ventilated Dogs(2022) Araos, Joaquin; Cruces, Pablo; Martin-Flores, Manuel; Donati, Pablo; Gleed, Robin D.; Boullhesen-Williams, Tomas; Perez, Agustin; Staffieri, Francesco; Retamal, Jaime; Melo, Marcos Vidal F.; Hurtado, Daniel E.The present study describes the magnitude and spatial distribution of lung strain in healthy anesthetized, mechanically ventilated dogs with and without positive end-expiratory pressure (PEEP). Total lung strain (LSTOTAL) has a dynamic (LSDYNAMIC) and a static (LSSTATIC) component. Due to lung heterogeneity, global lung strain may not accurately represent regional total tissue lung strain (TSTOTAL), which may also be described by a regional dynamic (TSDYNAMIC) and static (TSSTATIC) component. Six healthy anesthetized beagles (12.4 +/- 1.4 kg body weight) were placed in dorsal recumbency and ventilated with a tidal volume of 15 ml/kg, respiratory rate of 15 bpm, and zero end-expiratory pressure (ZEEP). Respiratory system mechanics and full thoracic end-expiratory and end-inspiratory CT scan images were obtained at ZEEP. Thereafter, a PEEP of 5 cmH(2)O was set and respiratory system mechanics measurements and end-expiratory and end-inspiratory images were repeated. Computed lung volumes from CT scans were used to evaluate the global LSTOTAL, LSDYNAMIC, and LSSTATIC during PEEP. During ZEEP, LSSTATIC was assumed zero; therefore, LSTOTAL was the same as LSDYNAMIC. Image segmentation was applied to CT images to obtain maps of regional TSTOTAL, TSDYNAMIC, and TSSTATIC during PEEP, and TSDYNAMIC during ZEEP. Compliance increased (p = 0.013) and driving pressure decreased (p = 0.043) during PEEP. PEEP increased the end-expiratory lung volume (p < 0.001) and significantly reduced global LSDYNAMIC (33.4 +/- 6.4% during ZEEP, 24.0 +/- 4.6% during PEEP, p = 0.032). LSSTATIC by PEEP was larger than the reduction in LSDYNAMIC; therefore, LSTOTAL at PEEP was larger than LSDYNAMIC at ZEEP (p = 0.005). There was marked topographic heterogeneity of regional strains. PEEP induced a significant reduction in TSDYNAMIC in all lung regions (p < 0.05). Similar to global findings, PEEP-induced TSSTATIC was larger than the reduction in TSDYNAMIC; therefore, PEEP-induced TSTOTAL was larger than TSDYNAMIC at ZEEP. In conclusion, PEEP reduced both global and regional estimates of dynamic strain, but induced a large static strain. Given that lung injury has been mostly associated with tidal deformation, limiting dynamic strain may be an important clinical target in healthy and diseased lungs, but this requires further study.
- ItemEffect of positive end expiratory pressure on lung injury and haemodynamics during experimental acute respiratory distress syndrome treated with extracorporeal membrane oxygenation and near-apnoeic ventilation(2021) Araos, Joaquin; Alegría Vargas, Leyla; Garcia, Aline; Cruces, Pablo; Soto Muñoz, Dagoberto Igor; Erranz, Benjamín; Salomon, Tatiana; Medina, Tania; García Valdes, Patricio Hernán; Dubo, Sebastian; Bachmann Barron, María Consuelo; Basoalto Escobar, Roque Ignacio; Valenzuela, Emilio Daniel; Rovegno Echavarría, Maximiliano David; Vera Alarcón, María Magdalena; Retamal Montes, Jaime; Cornejo Rosas, Rodrigo Alfredo; Bugedo Tarraza, Guillermo; Bruhn, AlejandroBackground: Lung rest has been recommended during extracorporeal membrane oxygenation (ECMO) for severe acute respiratory distress syndrome (ARDS). Whether positive end-expiratory pressure (PEEP) confers lung protection during ECMO for severe ARDS is unclear. We compared the effects of three different PEEP levels whilst applying near-apnoeic ventilation in a model of severe ARDS treated with ECMO. Methods: Acute respiratory distress syndrome was induced in anaesthetised adult male pigs by repeated saline lavage and injurious ventilation for 1.5 h. After ECMO was commenced, the pigs received standardised near-apnoeic ventilation for 24 h to maintain similar driving pressures and were randomly assigned to PEEP of 0, 10, or 20 cm H2O (n¼7 per group). Respiratory and haemodynamic data were collected throughout the study. Histological injury was assessed by a pathologist masked to PEEP allocation. Lung oedema was estimated by wet-to-dry-weight ratio. Results: All pigs developed severe ARDS. Oxygenation on ECMO improved with PEEP of 10 or 20 cm H2O, but did not in pigs allocated to PEEP of 0 cm H2O. Haemodynamic collapse refractory to norepinephrine (n¼4) and early death (n¼3) occurred after PEEP 20 cm H2O. The severity of lung injury was lowest after PEEP of 10 cm H2O in both dependent and non-dependent lung regions, compared with PEEP of 0 or 20 cm H2O. A higher wet-to-dry-weight ratio, indicating worse lung injury, was observed with PEEP of 0 cmH2O. Histological assessment suggested that lung injury was minimised with PEEP of 10 cm H2O. Conclusions: During near-apnoeic ventilation and ECMO in experimental severe ARDS, 10 cm H2O PEEP minimised lung injury and improved gas exchange without compromising haemodynamic stability.
- ItemEvaluation of Lung Aeration and Respiratory System Mechanics in Obese Dogs Ventilated With Tidal Volumes Based on Ideal vs. Current Body Weight(2021) Araos, Joaquin; Lacitignola, Luca; de Monte, Valentina; Stabile, Marzia; Porter, Ian; Hurtado, Daniel E.; Perez, Agustin; Crovace, Antonio; Grasso, Salvatore; Martin-Flores, Manuel; Staffieri, FrancescoWe describe the respiratory mechanics and lung aeration in anesthetized obese dogs ventilated with tidal volumes (VT) based on ideal (VTi) vs. current (VTc) body weight. Six dogs with body condition scores >= 8/9 were included. End-expiratory respiratory mechanics and end-expiratory CT-scan were obtained at baseline for each dog. Thereafter, dogs were ventilated with VT 15 ml kg(-1) based on VTi and VTc, applied randomly. Respiratory mechanics and CT-scan were repeated at end-inspiration during VTi and VTc. Data analyzed with linear mixed models and reported as mean +/- SD or median [range]. Statistical significance p < 0.05. The elastance of the lung, chest wall and respiratory system indexed by ideal body weight (IBW) were positively correlated with body fat percentage, whereas the functional residual capacity indexed by IBW was negatively correlated with body fat percentage. At end-expiration, aeration (%) was: hyperaeration 0.03 [0.00-3.35], normoaeration 69.7 [44.6-82.2], hypoaeration 29.3 [13.6-49.4] and nonaeration (1.06% [0.37-6.02]). Next to the diaphragm, normoaeration dropped to 12 +/- 11% and hypoaeration increased to 90 +/- 8%. No differences in aeration between groups were found at end-inspiration. Airway driving pressure (cm H2O) was higher (p = 0.002) during VTc (9.8 +/- 0.7) compared with VTi (7.6 +/- 0.4). Lung strain was higher (p = 0.014) during VTc (55 +/- 21%) than VTi (38 +/- 10%). The stress index was higher (p = 0.012) during VTc (SI = 1.07 [0.14]) compared with VTi (SI = 0.93 [0.18]). This study indicates that body fat percentage influences the magnitude of lung, chest wall, and total respiratory system elastance and resistance, as well as functional residual capacity. Further, these results indicate that obese dogs have extensive areas of hypoaerated lungs, especially in caudodorsal regions. Finally, lung strain and airway driving pressure, surrogates of lung deformation, are higher during VTc than during VTi, suggesting that in obese anesthetized dogs, ventilation protocols based on IBW may be advantageous.
- ItemModelo de instilación intra-traqueal de jugo gástrico en la rata: Curso temporal de las alteraciones histológicas(2014) Araos, Joaquin; Contreras, Rafael; Cutino, Andrea; Meneses, Manuel; Borzone, Gisella
- ItemOptimization of lung ventilation and perfusion in anesthetized horses using a ventilation mode with flow-limited expiration(2024) Araos, Joaquin; Driessen, Bernd; Brandly, Jerrianne; Gorenberg, Emma; Heerdt, Paul; Bruhn, Alejandro; Martin-Flores, Manuel; Adler, Andy; Hopster, KlausOBJECTIVE To investigate the mechanisms underlying the improved arterial oxygenation described with flow-limited expiration (FLEX) ventilation in anesthetized horses. ANIMALS 5 healthy adult research horses. METHODS Horses underwent volume-controlled ventilation for 60 minutes (VCV (1) ), followed by 60 minutes of FLEX, and 30 minutes of VCV (VCV (2) ). Main outcomes included the arterial partial pressure of oxygen-to-F io(2) (PF) ratio and electrical impedance tomography (EIT)-derived functional indices at the end of each phase. The EIT data were used to create regional maps of relative lung ventilation and perfusion as well as regional maps of ventilation/perfusion (V/Q) ratios. Ventilation indices derived from EIT included the fraction of expired volume in 1 second (FEV (1) ; %) and the time it took for the EIT signal to drop to 50% of the peak signal at end -inspiration (T- Close 50; seconds). Data were analyzed with 2-way ANOVA for repeated measures. P < .05 was considered significant. RESULTS The PF ratio increased significantly with FLEX compared to both VCV (1) and VCV (2) ( P < .01). There were no differences in the relative distribution of ventilation nor perfusion between ventilation strategies. However, when ventilation and perfusion were superimposed and V/Q ratio maps were constructed, FLEX had a homogenizing effect toward values of 1.0. The FEV (1) was shorter ( P < .01) and the T Close 50 was longer ( P < .001) in all regions during FLEX compared to both VCV (1) and VCV (2) . CLINICAL RELEVANCE Our findings suggest that FLEX ventilation in anesthetized horses enhances regional V/Q matching, likely by pro - longing expiratory aeration and reducing airway closure.
- ItemThree-dimensional electrical impedance tomography (EIT) to study regional ventilation/perfusion (V/Q̇) ratios in anesthetized pigs(Research Square, 2023) Larrabee, Shannon; Nugen, Sarah; Bruhn, Alejandro; Stowe, Symon; Adler, Andy; Martin-Flores, Manuel; Araos, JoaquinThis study aimed to develop a 3D method for assessing V/Q̇ ratios in a pig model of hemodynamic perturbations using electrical impedance tomography (EIT). In order to evaluate whether the physiological coherence of changes in EIT-derived V/Q̇ ratios, global EIT-derived V/Q̇ mismatch were compared with global gold-standards. The study found regional heterogeneity in the distribution of V/Q̇ ratios in both the ventrodorsal and craniocaudal directions. Although global EIT-derived indices of V/Q̇ mismatch consistently underestimated both low and high V/Q̇ mismatch compared to global gold standards, the direction of the change was similar. We have made the software available at no cost for other researchers to use. Future studies should compare regional V/Q̇ ratios determined by our method against other regional, high-resolution methods such as SPECT or PET scan.
- ItemThree-dimensional electrical impedance tomography to study regional ventilation/perfusion ratios in anesthetized pigs(2023) Larrabee, Shannon; Nugen, Sarah; Bruhn, Alejandro; Porter, Ian; Stowe, Symon; Adler, Andy; Martin-Flores, Manuel; Araos, JoaquinThis study aimed to develop a three-dimensional (3-D) method for assessing ventilation/perfusion (V/Q) ratios in a pig model of hemodynamic perturbations using electrical impedance tomography (EIT). To evaluate the physiological coherence of changes in EIT-derived V/Q ratios, global EIT-derived V/Q mismatches were compared with global gold standards. The study found regional heterogeneity in the distribution of V/Q ratios in both the ventrodorsal and craniocaudal directions. Although global EIT-derived indices of V/Q mismatch consistently underestimated both low and high V/Q mismatch compared with global gold standards, the direction of the change was similar. We made the software available at no cost for other researchers to use. Future studies should compare regional V/Q ratios determined by our method against other regional, high-resolution methods.