Browsing by Author "Barbosa, Angelica Mercia Pascon"

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    Maternal care of the whole litter improves the success rate of diabetes in pregnancy in rats
    (2022) Floriano, Juliana Ferreira; Barbosa, Angelica Mercia Pascon; de Oliveira, Rafael Guilen; Vega, Sofia; Catinelli, Bruna Bologna; Garcia, Gabriela Azevedo; Reyes, David Rafael; Sobrevia, Luis; Rudge, Marilza Vieira Cunha; DIAMATER Study Grp
    Induction of diabetes mellitus by streptozotocin (STZ) in rats at birth is of high mortality and low success rate when male puppies are separated from females, prioritizing females breastfeeding. Cross-parental care of the entire litter and SZT-induced diabetes up to 12 h post-birth become with high success rate, low animal death, and females with glycaemia > 140 mg/dL on the 90 postnatal day. Cross-parental care is more effective in STZ-induction of diabetes, which is maintained during pregnancy (diabetes in pregnancy), than the conventional protocol of male separation at birth.
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    Role of dietary food intake patterns, anthropometric measures, and multiple biochemical markers in the development of pregnancy- specific urinary incontinence in gestational diabetes mellitus
    (2024) Costa, Sarah Maria Barneze; Hallur, Raghavendra Lakshmana Shetty; Postdoc, David Rafael Abreu Reyes; Postdoc, Juliana Ferreira Floriano; Carvalhaes, Maria Antonieta de Barros Leite; Nunes, Helio Rubens de Carvalho; Sobrevia, Luis; Valero, Paola; Barbosa, Angelica Mercia Pascon; Rudge, Marilza Cunha Vieira
    Objectives: The aim of this study was to assess maternal dietary food intake patterns, anthropometric measures, and multiple biochemical markers in women with gestational diabetes mellitus and pregnancy-specific urinary incontinence and to explore whether antedating gestational diabetes mellitus environment affects the pregnancy-specific urinary incontinence development in a cohort of pregnant women with gestational diabetes mellitus and pregnancy-specific urinary incontinence. Methods: Maternal dietary information and anthropometric measurements were collected. At 24 wk of gestation, with a fasting venipuncture sample, current blood samples for biochemical markers of hormones, vita -mins, and minerals were analyzed. The groups were compared in terms of numerical variables using analysis of variance for independent samples followed by multiple comparisons. Results: Of the 900 pregnant women with complete data, pregnant women in the gestational diabetes mellitus pregnancy-specific urinary incontinence group had higher body mass index during pregnancy, arm circumference, and triceps skinfold than the non-gestational diabetes mellitus continent and non-gestational diabetes mellitus pregnancy-specific urinary incontinence groups, characterizing an obesogenic maternal environment. Regarding dietary food intake, significant increases in aromatic amino acids, branched-chain amino acids, dietary fiber, magnesium, zinc, and water were observed in pregnancy-specific urinary incontinence group compared with the non-gestational diabetes mellitus continent group. Serum vitamin C was reduced in the gestational diabetes mellitus pregnancy-specific urinary incontinence group compared with the non-gestational diabetes mellitus pregnancy-specific urinary incontinence group. Conclusions: This study emphasizes the necessity for a comprehensive strategy for gestational diabetes melli-tus women with pregnancy-specific urinary incontinence in terms of deviation in maternal adaptation trend-ing toward obesity and maternal micronutrients deficiencies. (c) 2023 Elsevier Inc. All rights reserved.
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    Transcriptomic Profiling of Rectus Abdominis Muscle in Women with Gestational Diabetes-Induced Myopathy: Characterization of Pathophysiology and Potential Muscle Biomarkers of Pregnancy-Specific Urinary Incontinence
    (2022) Alves, Fernanda Cristina Bergamo; de Oliveira, Rafael Guilen; Reyes, David Rafael Abreu; Garcia, Gabriela Azevedo; Floriano, Juliana Ferreira; Shetty, Raghavendra Hallur Lakshmana; Mareco, Edson Assuncao; Dal-Pai-Silva, Maeli; Payao, Spencer Luiz Marques; Souza, Fatima Pereira de; Witkin, Steven S.; Sobrevia, Luis; Barbosa, Angelica Mercia Pascon; Rudge, Marilza Vieira Cunha
    Gestational diabetes mellitus (GDM) is recognized as a "window of opportunity" for the future prediction of such complications as type 2 diabetes mellitus and pelvic floor muscle disorders, including urinary incontinence and genitourinary dysfunction. Translational studies have reported that pelvic floor muscle disorders are due to a GDM-induced-myopathy (GDiM) of the pelvic floor muscle and rectus abdominis muscle (RAM). We now describe the transcriptome profiling of the RAM obtained by Cesarean section from GDM and non-GDM women with and without pregnancy-specific urinary incontinence (PSUI). We identified 650 genes in total, and the differentially expressed genes were defined by comparing three control groups to the GDM with PSUI group (GDiM). Enrichment analysis showed that GDM with PSUI was associated with decreased gene expression related to muscle structure and muscle protein synthesis, the reduced ability of muscle fibers to ameliorate muscle damage, and the altered the maintenance and generation of energy through glycogenesis. Potential genetic muscle biomarkers were validated by RT-PCR, and their relationship to the pathophysiology of the disease was verified. These findings help elucidate the molecular mechanisms of GDiM and will promote the development of innovative interventions to prevent and treat complications such as post-GDM urinary incontinence.