Browsing by Author "Barrales, Luis"

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    Direct and indirect in vitro organogensis of Leucocoryne purpurea (Alliaceae) a chilean ornamental geophyte
    (SEJANI PUBL, 2008) Escobar, Luis H.; Jordan, Miguel; Olate, Eduardo; Barrales, Luis; Gebauer, Marlene
    In vitro shoot induction from callus and direct bulbil formation from shoots were obtained in Leucocoryne purpurea, an endemic vulnerable Chilean species. Basal leaf portions, bulb basal plate and root tips from in vitro gown plants were used as explants. Only bulb basal plate explants displayed morphogenic responses. Callus, shoot and bulbils developed on MS basal medium in the presence of various growth regulator combinations. Callus formation from bulb basal explants ranged between 40 to 100%; the highest response was observed in the presence of 4.5 or 13.6 mu M 2,4-D alone or 4.5 mu M 2,4-D in combination with 0.5 mu M BA after 4 months. Picloram alone or in combination with 2iP or BAP also supported callus initiation. Indirect shoot formation from callus occurred only after a further culture period of 2 months in MS medium containing 0.5 or 2.3 mu M TDZ. Basal bulb plate explants cultured in the presence of TDZ alone (0.5 and 2.3 RM, respectively) exhibited direct shoot formation within 90 days. After subculture in the presence of 0.5 mu M TDZ each single shoot formed new shoots (3 to 4) and new bulbils. However higher TDZ levels, up to 2.3 mu M, produced shoots with roots only, inhibiting bulbil formation, and the 4.5 mu M TDZ concentration caused necrosis.
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    FEEDING DRY OLIVE CAKE MODIFIES SUBCUTANEOUS FAT COMPOSITION IN LAMBS, NOTING CAKE RESISTANCE TO DEGRADATION AND PEROXIDATION
    (INST INVESTIGACIONES AGROPECUARIAS, 2009) Vera, Raul; Aguilar, Claudio; Lira, Rodrigo; Toro, Paula; Barrales, Luis; Pena, Ivan; Squella, Fernando; Perez, Patricio; Quenaya, Javiera; Yutronic, Hanja; Briones, Ignacio
    The purpose of this experiment was to determine the extent to which lamb carcass quality and fat composition could be altered by applying a dry olive cake-based ration instead of a conventional ration or pasture feeding. Three treatments were compared in a completely randomized experiment using 36 single male Suffolk Down lambs with an initial age and live weight of 80 d and 25 kg, respectively. The three treatments were: (a) suckling lambs kept with their mothers on annual Mediterranean grassland (GRAZE); (b) weaned stall-fed lambs on a control ration (CONC); and (c) weaned stall-fed lambs on an olive cake-based ration (CAKE). At the end of the 28-d experimental period, lambs were slaughtered, carcass quality evaluated, and samples of subcutaneous fat analyzed for fatty acids. Treatments did not differ in weight gain or carcass weight and quality, but highly significant differences were found in several fatty acid contents. CAKE animals had reduced palmitic acid as well as increased oleic and stearic acid contents. Atherogenic and thrombogenic indices improved with cake feeding. Principal component and discriminant analyses clearly differentiated treatments suggesting they could be applicable for traceability purposes. A laboratory experiment demonstrated that with or without added vitamin E, the dry olive cake did not alter its lipid properties with increased storage time.
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    Nutrient absorption, feed intake and liveweight change as a consequence of Roux - en - Y gastric bypass with increasing alimentary limb lengths in the pig model.
    (2011) Gandarillas Henríquez, Mónica; Boza, Camilo; Barrales, Luis; Hodgkinson, Suzanne Marie; Bas Mir, Fernando