Browsing by Author "Bertin, P"

Now showing 1 - 5 of 5
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    Analysis of the complementary determining region III of the immunoglobulin heavy chain locus in acute lymphoblastic leukemia in Chilean children
    (1996) Barriga, FJ; Risueno, C; Patillo, JC; Andrade, W; Cabrera, ME; Beressi, V; DelBorgo, P; Salgado, C; Becker, A; Campbell, M; Bertin, P
    We have analyzed the sequence of 40 VDJ rearrangements of the immunoglobulin heavy chain gene locus on 32 unselected children from Chile with precursor B cell ALL at diagnosis. Rearrangements were derived by PCR with VH gene family-specific primers and sequenced directly. The number of VDJ rearrangements, and the pattern of VH, DH and JH gene usage was identical to the one reported by groups from developed countries. CDR3 regions represented an unbiased repertoire; VH to JH joinings were in frame in 36% of cases. Absent N nucleotides in the DJ border, suggestive of fetal origin of ALL, were seen in 9/40 rearrangements but they did not correlate with younger age. More than one rearrangement was sequenced in six patients, representing independent events with no signs of clonal evolution. One patient was analyzed at first bone marrow relapse showing persistence of one rearrangement and evolution of a second one which conserved the DJ border. The subset of B cell precursors which suffer malignant transformation to ALL appear to be common in different parts of the world.
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    B-cell monoclonal lymphocytosis and B-cell abnormalities in the setting of familial B-cell chronic lymphocytic leukemia
    (2003) Marti, GE; Carter, P; Abbasi, F; Washington, GC; Jain, N; Zenger, VE; Ishibe, N; Goldin, L; Fontaine, L; Weissman, N; Sgambati, M; Fauget, G; Bertin, P; Vogt, RF; Slade, B; Noguchi, PD; Stetler-Stevenson, MA; Caporaso, N
    Background: Among all hematologic malignancies, B-cell chronic lymphocytic leukemia (BCLL) has the highest familial clustering (three- to sevenfold increase), strongly suggesting a genetic component to its etiology. Familial BCLL can be used as a model to study the early pathogenesis of this disease.
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    Natural killer cell leukemia. Case report
    (SOC MEDICA SANTIAGO, 2005) Guerrero, M; Lira, P; Bertin, P; Galleguillos, M; Ocqueteau, M
    Natural killer leukemia is a rare and highly aggressive neoplasm, is more common in young male patients and has a very poor prognosis, with a median survival of few weeks. We report a 17 years old male patient who developed, after an acute upper respiratory disease, a rapidly multiorganic failure with pancytopenia. Bone marrow aspiration and trephine biopsy showed an acute lymphoblastic leukemia. The immunophenotypic and immunohistochemistry revealed a natural killer acute leukemia. The disease progressed rapidly and the patient died shortly after the diagnosis.
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    Selective engraftment of the granulocyte compartment after allogeneic bone marrow transplantation in a patient with severe aplastic anemia
    (1996) Barriga, FJ; Legues, ME; Bertin, P
    Purpose: We present a patient with severe aplastic anemia who had partial engraftment with full chimerism after allogeneic bone marrow transplantation from an HLA identical sibling.
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    t(1;5)(q23;q33) in a patient with high-risk B-lineage acute lymphoblastic leukemia
    (1996) Barriga, F; Bertin, P; Legues, E; Risueno, C; Andrade, W; Cabrera, E; Grebe, G
    The t(1;5)(q23;q33) is a rare genetic anomaly that was reported previously in two infants with a myeloproliferative disorder and eosinophilia and in one adult patient with acute nonlymphocytic leukemia (ANLL). A 13-year-old boy with high-risk early pre-B acute lymphoblastic leukemia (ALL) who presented to our institution carried the t(1;5)(q23;q33). He had an initial blast count of 230 x 10(9)/L and responded poorly to prednisone. Complete remission ICR) was achieved, and he had a bone marrow (BM) relapse 3 months after despite intensive consolidation therapy He underwent allogeneic BM transplantation (BMT) from a human leukocyte antigen (HLA)-identical sibling in early relapse with total body irradiation (TBI) and cyclophosphamide conditioning. He had a short second CR with a central nervous system (CNS) relapse on day +106 after BMT. Two of the previously reported patients also did not respond to chemotherapy. The t(1;5)(q23;q33) appears to be a rare lineage nonspecific anomaly related to hematologic malignancies that are resistant to current therapy.