Browsing by Author "Brenet, Marianne"

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    A First in Human Trial Implanting Microalgae Shows Safety of Photosynthetic Therapy for the Effective Treatment of Full Thickness Skin Wounds
    (FRONTIERS MEDIA SA, 2021) Obaid, Miguel Luis; Camacho, Juan Pablo; Brenet, Marianne; Corrales Orovio, Rocio; Carvajal Diaz, Felipe Alonso; Martorell, Ximena; Werner, Consuelo; Simon, Valeska; Varas, Juan; Calderon, Wilfredo; Guzman, Christian Dani; Bono, Maria Rosa; San Martin, Sebastián; Eblen Zajjur, Antonio Alejandro; Egaña Erazo, José Tomás
    Insufficient oxygen supply represents a relevant issue in several fields of human physiology and medicine. It has been suggested that the implantation of photosynthetic cells can provide oxygen to tissues in the absence of a vascular supply. This approach has been demonstrated to be successful in several in vitro and in vivo models; however, no data is available about their safety in human patients. Here, an early phase-1 clinical trial ( identifier: NCT03960164, ) is presented to evaluate the safety and feasibility of implanting photosynthetic scaffolds for dermal regeneration in eight patients with full-thickness skin wounds. Overall, this trial shows that the presence of the photosynthetic microalgae Chlamydomonas reinhardtii in the implanted scaffolds did not trigger any deleterious local or systemic immune responses in a 90 days follow-up, allowing full tissue regeneration in humans. The results presented here represent the first attempt to treat patients with photosynthetic cells, supporting the translation of photosynthetic therapies into clinics.
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    Case report: Long-term follow-up of a large full-thickness skin defect treated with a photosynthetic scaffold for dermal regeneration
    (FRONTIERS MEDIA SA, 2022) Obaid, Miguel L.; Carvajal Diaz, Felipe Alonso; Camacho, Juan Pablo; Corrales-Oravio, Rocio; Martorell, Ximena; Varas, Juan; Calderon, Wilfredo; Dani Guzman, Christian; Brenet, Marianne; Castro, Margarita; Orlandi, Cecilia; San Martin, Sebastian; Eblen Zajjur Antonio Alejandro; Egaña Erazo, José Tomás
    It is broadly described that almost every step of the regeneration process requires proper levels of oxygen supply; however, due to the vascular disruption in wounds, oxygen availability is reduced, being detrimental to the regeneration process. Therefore, the development of novel biomaterials combined with improved clinical procedures to promote wound oxygenation is an active field of research in regenerative medicine. This case report derives from a cohort of patients enrolled in a previously published ongoing phase I clinical trial (NCT03960164), to assess safety of photosynthetic scaffolds for the treatment of full skin defects. Here, we present a 56 year old patient, with a scar contracture in the cubital fossa, which impaired the elbow extension significantly affecting her quality of life. As part of the treatment, the scar contracture was removed, and the full-thickness wound generated was surgically covered with a photosynthetic scaffold for dermal regeneration, which was illuminated to promote local oxygen production. Then, in a second procedure, an autograft was implanted on top of the scaffold and the patient's progress was followed for up to 17 months. Successful outcome of the whole procedure was measured as improvement in functionality, clinical appearance, and self-perception of the treated area. This case report underscores the long-term safety and applicability of photosynthetic scaffolds for dermal regeneration and their stable compatibility with other surgical procedures such as autograft application. Moreover, this report also shows the ability to further improve the clinical outcome of this procedure by means of dermal vacuum massage therapy and, more importantly, shows an overall long-term improvement in patient & PRIME;s quality of life, supporting the translation of photosynthetic therapies into human patients.