Browsing by Author "Catrileo, Daniela"
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- ItemEffect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480(2021) Catrileo, Daniela; Moreira, Sandra; Angelica Ganga, Maria; Godoy, LilianaBrettanomyces bruxellensis is considered the most significant contaminant yeast in the wine industry since it causes a deterioration in the organoleptic properties of the wine and significant economic losses. This deterioration is due to the production of volatile phenols from hydroxycinnamic acids. These compounds possess antimicrobial properties; however, B. bruxellensis can resist this effect because it metabolizes them into less toxic ones. Recent studies have reported that B. bruxellensis grows under different stress conditions, including p-coumaric acid (pCA) but effective methods for its control have not been found yet. Since that in other yeasts, such as Saccharomyces cerevisiae, it has been described that light affects its growth, and we evaluated whether the light would have a similar effect on B. bruxellensis. The results show that at light intensities of 2,500 and 4,000 lux in the absence of pCA, B. bruxellensis LAMAP2480 does not grow in the culture medium; however, when the medium contains this acid, the yeast adapts to both factors of stress managing to grow. The expression of genes related to oxidative stress in B. bruxellensis LAMAP2480, such as SOD1, GCN4, and ESBP6, showed a higher relative expression when the yeast was exposed to 2,500 lux compared to 4,000 lux, agreeing with the growth curves. This suggests that a higher expression of the genes studied would be related to stress-protective effects by pCA.
- ItemEl cielo será como un río. Poemas y cantos de sanadoras, guerreras y guardianas de la naturaleza de Chile plurinacional(2022) Arriagada Vladilo, Mirka; Avendaño Curaqueo, Elisa; Romero Cheuquian, Catalina; Catrileo, Daniela; Río, Alejandra del; Díaz, Victoria; Domínguez, Delia; Fariña, Soledad; Figuero, Damsi; Hernández, Elvira; Manquepillán Calfuleo, Faumelisa; Millaleo, Daniela; Miranda Rupailaf, Roxana; Mistral, Gabriela; Muñoz, Rosabetty; Olivares, Alicia de las Mercedes; Paredes Pinda, Adriana; Sánchez, Isidora; Urriola, Malú; Vicuña, Cecilia; Barros Cruz, Maria José; Carreño Bolívar, RubíCantos y poemas de mujeres de todos los territorios de Chile se reúnen en esta antología que nos invita a repensar nuestra relación con la naturaleza desde los principios de la reciprocidad y la dignidad. Así, se nos revelan saberes femeninos, indígenas, campesinos y populares a través de textos que ingresan al terreno de lo político para disputar la lógica predatoria promovida por el capitalismo y su idea de naturaleza como una materia inerte e inhabitada que debe ser colonizada y explotada con fines comerciales. Sigue la voz de estas sanadoras, guardianas y guerreras junto a las ballenas, shumpalles, pájaros y helechos en este canto a Chile plurinacional.
- Item“Ojo de agua atenta”: Aparatos de resonancia y resistencia en los videoperformances de Paula Coñoepan y Sebastian Calfuqueo(2022) Amaro, Lorena; Catrileo, Daniela; Quevedo, JavieraEn las dos últimas décadas urge la necesidad de pensar nuevas formas de relación con el planeta, marcadas en la modernidad por el afán de dominio y control. El colonialismo impuso una «mirada extractiva» (Gómez-Barris) sobre los territorios anexados a las metrópolis; visión y poder se combinan en diversas metáforas cognitivas que revelan, además de la centralidad de la visión en la cultura occidental, la postergación de formas perceptivas y cognitivas que pudieran propiciar vínculos más emancipadores, «tentaculares» (Haraway) e integradores en tiempos de crisis planetaria. En este artículo abordamos videoperformances de dos artistas visuales mapuche, Sebastián Calfuqueo y Paula Coñoepan, cuyo quehacer corporizado explora las posibilidades del ojo y, sobre todo, del oído, invitándonos a viajar por superficies y paisajes no normados, en que el uso del sonido líquido, ambiental, funciona como aparato de resonancia y resistencia de genealogías, cuerpos y subjetividades.
- ItemSolicoccozyma aeria YCPUC79 Promotes Tomato Seedling Root Growth by Volatile Organic Compounds Emission(2024) Carvajal Contardo, Maria José; Albornoz Gutierrez, Francisco Javier; Catrileo, Daniela; Chorbadjian Alonso, Rodrigo Armen; Gebauer Hernández, Marlene Gloria; Godoy Olivares, LilianaBeneficial microorganisms promote plant growth through different mechanisms, such asthe production of plant hormones, the synthesis of 1-aminocyclopropane-1-carboxylic aciddeaminase (ACCD), or, through volatile organic compounds (VOCs) emission. There are numerousstudies describing a biostimulant action mediated by VOCs released by bacteria and filamentousfungi. However, in soil yeasts, research in this regard is still incipient. In a previous study, wepresent the stimulatory action of Solicoccozyma aeria YCPUC79 on tomato seedling growth byinoculating the rootzone with this yeast. The positive effect of S. aeria was related to the synthesis ofindole acetic acid (IAA) and the presence of ACCD activity by the yeast. In the present study, weevaluated whether S. aeria is capable of emitting VOCs with biostimulant activity. For this, anexperiment was conducted to test the release of VOCs in four treatments: S. aeria (Sa), tomatoseedlings (T), tomato seedlings sharing the ambient with S. aeria but with no physical contact (TSa)plus a control with no yeast nor tomato seedlings (C). Tomato seedlings exposed to S. aeria inoculum(TSa) presented 1.2-fold shorter main roots but increased the number of lateral roots by 80%compared to T. Regarding the analysis of VOCs, 59 compounds were identified excluding thosefound in the control treatment. These compounds represent twelve chemical families, includingalcohols, esters, furans, hydrocarbons, ketones and terpenes. The treatment TSa shows an increasedabundance of ketones, alcohols, esters, sulfur-containing compounds, and pyrazines in comparisonto T treatment. Three compounds (butyl hept-4-yl ester-phthalic acid, (E)1.3-pentadiene and 1-propenylthiol) were exclusively present in the TSa treatment. This study provides, for the first time,information on a soil yeast capable of promoting the production of lateral roots in tomato throughVOCs.
- ItemVolatile organic compounds produced after exposure of tomato roots to the soil yeast Solicoccozyma terrea modulate root nitrate transporters in tomato(2025) Albornoz Gutiérrez, Francisco Javier; Carvajal Contardo, Mariajose; Catrileo, Daniela; Gebauer Hernández, Marlene Gloria; Godoy Olivares, LilianaBackground Beneficial microorganisms modify root architecture through different mechanisms mostly related to plant hormones synthesis. Less information is available regarding the emission of microbial volatile organic compounds (VOCs) and their effects on root architecture. Numerous studies describe the release of VOCs by bacteria and filamentous fungi, but little information is available regarding soil yeasts.MethodsThe VOCs released by the soil yeast Solicoccozyma terrea were evaluated in an experiment with tomato seedlings. Four treatments (tomato seedlings, T; S. terrea inoculum without tomato seedlings, St; tomato seedlings exposed to the volatiles of S. terrea inoculum, TSt; and a control without tomato seedlings or yeast inoculum, C) were assessed in terms of root morphology. VOCs were analyzed by GC-MS, and in a second experiment, the response of tomato seedlings and root nitrate uptake transporters to specific compounds identified in the first experiment was evaluated under low and high N supply.ResultsA mixture with 57 VOCs was identified which promoted lateral roots formation in tomato plants. Three compounds (isopropyl alcohol, 2-pentylfuran and trans-1,3-pentadiene) were exclusively present in the TSt treatment. All three compounds stimulated lateral roots formation and increased root volume. These compounds showed a stimulatory effect on the expression of NRT1.2, NRT2.1 and NRT2.3 nitrate transporters under N deficiency conditions.ConclusionsThis study provides, for the first time, information on a soil yeast capable of promoting lateral roots formation in tomato through VOCs and that isopropyl alcohol, 2-pentylfuran and trans-1,3-pentadiene modulate root nitrate transporters expression under N deficiency conditions.