Browsing by Author "Chirinos, Rosana"

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    A multiomics integrative analysis of color de-synchronization with softening of 'Hass' avocado fruit: A first insight into a complex physiological disorder
    (2023) Nunez-Lillo, Gerardo; Ponce, Excequel; Arancibia-Guerra, Camila; Carpentier, Sebastien; Carrasco-Pancorbo, Alegria; Olmo-Garcia, Lucia; Chirinos, Rosana; Campos, David; Campos-Vargas, Reinaldo; Meneses, Claudio; Pedreschi, Romina
    Exocarp color de-synchronization with softening of 'Hass' avocado is a relevant recurrent problem for the avocado supply chain. This study aimed to unravel the mechanisms driving this de-synchronization integrating omics datasets from avocado exocarp of different storage conditions and color phenotypes. In addition, we propose potential biomarkers to predict color synchronized/de-synchronized fruit. Integration of transcriptomics, proteomics and metabolomics and network analysis revealed eight transcription factors associated with differentially regulated genes between regular air (RA) and controlled atmosphere (CA) and twelve transcription factors related to avocado fruit color de-synchronization control in ready-to-eat stage. CA was positively correlated to auxins, ethylene, cytokinins and brassinosteroids-related genes, while RA was characterized by enrichment of cell wall remodeling and abscisic acid content associated genes. At ready-to-eat higher contents of flavonoids, abscisic acid and brassinosteroids were associated with color-softening synchronized avocados. In contrast, de-synchronized fruit revealed increases of jasmonic acid, salicylic acid and auxin levels.
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    Atmospheric immersion and vacuum impregnation of gallotannins and hydrolysed gallotannins from tara pods (Caesalpinia spinosa) mitigate acrylamide and enhances the antioxidant power in potato chips
    (2024) Campos, David; Chirinos, Rosana; Huaraca-Espinoza, Paola; Aguilar-Galvez, Ana; Garcia-Rios, Diego; Pedreschi, Franco; Pedreschi, Romina
    This research aimed to evaluate atmospheric pressure immersion (API) and vacuum impregnation (VI) of non -hydrolyzed and hydrolyzed tara gallotannins (TE and THE) extracts and the purification process by leaching with ethyl acetate (TE-L and THE-L) and absorption chromatography on the acrylamide (AA) mitigation in potato chips. Better results were obtained with THE-L at hydrolysis degrees of 48.5 and 99.8 % and API with AA reduction of 57.9 and 61.7 %, respectively (p > 0.05), while with TE-L, AA reduction was 29.2 %. Instead, THE-L and TE-L with VI reduced AA by 70.3 and 66.4 %, respectively. In potato chips subjected to THE-L treatment, phenolic compounds and ABTS AC increased in 86.5-91.0 % and 71.0-103.2 %, respectively compared to the control. The main antioxidants were identified in the extracts and in potato chips. Tara gallotannins are an interesting alternative to mitigate AA formation and to enhance the antioxidant power of potato chips.
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    Postharvest storage and cooking techniques affect the stability of glucosinolates and myrosinase activity of Andean mashua tubers (Tropaeolum tuberosum)
    (2019) Campos, David; Aguilar-Galvez, Ana; Garcia-Rios, Diego; Chirinos, Rosana; Limaymanta, Evelin; Pedreschi, Romina
    The identification and quantification of Glucosinolates (Gls) via UPLC (R) MS-MS/PDA/qToF in ten mashua cultivars was carried out. Gls total contents were within the 4.9-54.2 mu molg(-1) dry matter range, of which 96-99% corresponded to glucoaubrietin. Other less abundant Gls were glucotropaeolin and tentatively two isomers of hydroxybenzyl Gls. Postharvest refrigeration and shade storage conditions increased the content of Gls up to day 6 in 39.7% and 51.5% respectively. Sun exposure increased the Gls content in 40% up to day 3 but from day 6 considerable losses were attained (92% at day 15). Low correlation levels (R-2) between the Gls and myrosinase (MYR) activity of 0.57, 0.28 and 0.39 for the refrigeration, shade and sun exposure treatments were obtained. The cooking regimes tested, boiling, microwaving and baking totally inactivated MYR without affecting the Gls content.
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    Tara pod (Caesalpinia spinosa) extract mitigates neo-contaminant formation in Chilean bread preserving their sensory attributes
    (2018) Pedreschi Plasencia, Franco; Saavedra, Ilse; Bunger, Andrea; Zúñiga, Rommy N.; Pedreschi, Romina; Chirinos, Rosana; Campos, David; Mariotti Celis, María Salomé