Browsing by Author "Dureja, H."

Now showing 1 - 3 of 3
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    Biological databases and tools for neurological disorders
    (IMR Press Limited, 2022) Usman, M.B.; Ojha, S.; Jha, S.K.; Chellappan, D.K.; Gupta, G.; Singh, S.K.; Dua, K.; Roychoudhury, S.; Kumar, N.; Khan, F.A.; Dureja, H.; Upadhye, V.; Zacconi, Flavia C. M.; Prasanna, P.; Kesari, K.K.; Ashraf, G.M.; Alexiou, A.; Jha, N.K.
    Computational approaches to study of neuronal impairment is rapidly evolving, as experiments and intuition alone do not explain the complexity of the brain system. An overwhelming increase in the amount of new data from both theory and computational modeling necessitate the development of databases and tools for analysis, visualization and interpretation of neuroscience data. To ensure the sustainability of this development, consistent update and training of young professionals is imperative. For this purpose, relevant articles, chapters, and modules are essential to keep abreast of developments. This review seeks to outline the biological databases and analytical tools along with their applications. It is envisaged that such knowledge could provide a ''training recipe'' for young scientists and a guide for professionals and researchers in neuroscience.
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    Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation
    (2021) Zacconi, Flavia C. M.; Chellappan, D. K.; Dureja, H.; Dua, K.; Raj Paudel, K. R.; Shastri, M. D.; Mehta, M.; Gulati, M.; Hansbro, P.; Singh, S. K.; Satija, S.; Shukla, S. D.
    Aim: In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (NQO1); gamma-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (Nox)-4; Nox2B) genes. Results: Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely Nox2B and Nox4, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and Nqo-1 in a dose-dependent manner. Conclusion: The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.
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    Targeting eosinophils in respiratory diseases: biological axis, emerging therapeutics and treatment modalities
    (2021) Lee, L.-Y.; Hew, G.S.Y.; Mehta, M.; Shukla, S.D.; Satija, S.; Khurana, N.; Anand, K.; Dureja, H.; Singh, S.K.; Mishra, V.; Singh, P.K.; Gulati, M.; Prasher, P.; Aljabali, A.A.A.; Tambuwala, M.M.; Thangavelu, L.; Panneerselvam, J.; Gupta, G.; Zacconi, Flavia C. M.; Shastri, M.; Jha, N.K.; Xenaki, D.; MacLoughlin, R.; Oliver, B.G.; Chellappan, D.K.; Dua, K.
    Eosinophils are bi-lobed, multi-functional innate immune cells with diverse cell surface receptors that regulate local immune and inflammatory responses. Several inflammatory and infectious diseases are triggered with their build up in the blood and tissues. The mobilization of eosinophils into the lungs is regulated by a cascade of processes guided by Th2 cytokine generating T-cells. Recruitment of eosinophils essentially leads to a characteristic immune response followed by airway hyperresponsiveness and remodeling, which are hallmarks of chronic respiratory diseases. By analysing the dynamic interactions of eosinophils with their extracellular environment, which also involve signaling molecules and tissues, various therapies have been invented and developed to target respiratory diseases. Having entered clinical testing, several eosinophil targeting therapeutic agents have shown much promise and have further bridged the gap between theory and practice. Moreover, researchers now have a clearer understanding of the roles and mechanisms of eosinophils. These factors have successfully assisted molecular biologists to block specific pathways in the growth, migration and activation of eosinophils. The primary purpose of this review is to provide an overview of the eosinophil biology with a special emphasis on potential pharmacotherapeutic targets. The review also summarizes promising eosinophil-targeting agents, along with their mechanisms and rationale for use, including those in developmental pipeline, in clinical trials, or approved for other respiratory disorders.