Browsing by Author "Fuentes, D."

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    First characterization of the gut microbiome associated with Mytilus chilensis collected at a mussel farm and from a natural environment in Chile
    (2022) Santibanez, P.; Romalde, J.; Maldonado, J.; Fuentes, D.; Figueroa, J.
    The gut microbiome of Mytilus chilensis was analyzed using a next-generation sequencing (NGS) approach using as target the V6-V8 16S rDNA gene regions. The resident gut bacterial communities were analyzed in individuals collected from two locations, both free-living in natural conditions (Wild Type group, WT) and those found in intensive commercial cultures at a Mussel Farm (MF group) off the Chilean coast. A total of 1.2 M of nonchimeric sequences were detected, belonging to 30 phyla, including Fusobacteria, Proteobacteria, Bacteroides, Tenericutes, and 404 genera taxa. The major fraction of the sequences detected corresponded to Vibrionaceae and Fusobacteriaceae families for MF and WT groups, respectively. The gut microbiome of M. chilensis showed a wide diversity of microorganisms; however, differences were observed depending on the origin of samples, where gut samples from the mussel farm showed a lower number of genera when compared to gut tissue samples collected from the wild type individuals. The dominant bacterial are Vibrio, Psychrilyobacter, Mycoplasma, and Psychromonas, that were present with different relative abundancies among the samples analyzed. This next generation sequencing technique, applied for the first time in Chilean mussel (M. chilensis), showed that the gut is a useful tissue for the study of bacterial communities in this bivalve mollusk. The results obtained in this work provided insights into the composition of the microbiota of M. chilensis, unravelling the great bacterial diversity and the effect of habitat on the bacterial community structure.
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    Inversion recovery and saturation recovery pulmonary vein MR angiography using an image based navigator fluoro trigger and variable-density 3D cartesian sampling with spiral-like order
    (Springer Science and Business Media B.V., 2024) Craft, J.; Weber, J.; Li;Y.; Cheng, J.Y.; Diaz , N.; Kunze, K.P.; Schmidt, M.; Grgas, M.; Weber, S.; Tang, J.; Parikh, R.; Onuegbu, A.; Yamashita, A.-M.; Haag, E.; Fuentes, D.; Czipo, M.; Neji, R.; Espada, C.B.; Prieto Vasquez, Claudia Del Carmen; Botnar, Rene Michael
    Contrast enhanced pulmonary vein magnetic resonance angiography (PV CE-MRA) has value in atrial ablation pre-procedural planning. We aimed to provide high fidelity, ECG gated PV CE-MRA accelerated by variable density Cartesian sampling (VD-CASPR) with image navigator (iNAV) respiratory motion correction acquired in under 4 min. We describe its use in part during the global iodinated contrast shortage. VD-CASPR/iNAV framework was applied to ECG-gated inversion and saturation recovery gradient recalled echo PV CE-MRA in 65 patients (66 exams) using.15 mmol/kg Gadobutrol. Image quality was assessed by three physicians, and anatomical segmentation quality by two technologists. Left atrial SNR and left atrial/myocardial CNR were measured. 12 patients had CTA within 6 months of MRA. Two readers assessed PV ostial measurements versus CTA for intermodality/interobserver agreement. Inter-rater/intermodality reliability, reproducibility of ostial measurements, SNR/CNR, image, and anatomical segmentation quality was compared. The mean acquisition time was 3.58 ± 0.60 min. Of 35 PV pre-ablation datasets (34 patients), mean anatomical segmentation quality score was 3.66 ± 0.54 and 3.63 ± 0.55 as rated by technologists 1 and 2, respectively (p = 0.7113). Good/excellent anatomical segmentation quality (grade 3/4) was seen in 97% of exams. Each rated one exam as moderate quality (grade 2). 95% received a majority image quality score of good/excellent by three physicians. Ostial PV measurements correlated moderate to excellently with CTA (ICCs range 0.52–0.86). No difference in SNR was observed between IR and SR. High quality PV CE-MRA is possible in under 4 min using iNAV bolus timing/motion correction and VD-CASPR. Graphical Abstract: (Figure presented.)
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    RENAL FUNCTION IN THE PERIOPERATIVE PERIOD OF CARDIAC SURGERY WITH CARDIOPULMONARY BYPASS, IN NEWBORNS WITH COMPLEX CONGENITAL HEART DISEASES: USE OF THE BIOMARKER KIM-1.
    (LIPPINCOTT WILLIAMS & WILKINS, 2016) Borchert, E.; Lema, G.; Jalil, R.; Guzman, A. M.; De la Fuente, R.; Gomez, M.; Fuentes, D.
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    Uso de pacientes estandarizados para la adquisición de conocimientos teórico-clínicos en estudiantes de pregrado
    (2015) Dois C., Angelina M.; Sanhueza, M.; Fuentes, D.; Farías Cancino, Angélica