Browsing by Author "Gallardo, Victoria"

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    Diseño y medición experimental del desempeño de Ruta CL, estándar tipográfico para las carreteras de Chile
    (2015) Ramírez Montecinos, Rodrigo Fernando; Gallardo, Victoria
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    Insulin-Stimulated L-Arginine Transport Requires SLC7A1 Gene Expression and Is Associated With Human Umbilical Vein Relaxation
    (WILEY, 2011) Gonzalez, Marcelo; Gallardo, Victoria; Rodriguez, Natalia; Salomon, Carlos; Westermeier, Francisco; Guzman Gutierrez, Enrique; Abarzua, Fernando; Leiva, Andrea; Casanello, Paola; Sobrevia, Luis
    Insulin causes endothelium-derived nitric oxide (NO)-dependent vascular relaxation, and increases L-arginine transport via cationic amino acid transporter 1 (hCAT-1) and endothelialNOsynthase (eNOS) expression and activity in human umbilical vein endothelium (HUVEC). We studied insulin effect on SLC7A1 gene (hCAT-1) expression and hCAT-transport activity role in insulin-modulated human fetal vascular reactivity. HUVEC were used for L-arginine transport and L-[H-3] citrulline formation (NOS activity) assays in absence or presence of N-ethylmaleimide (NEM) or L-lysine (L-arginine transport inhibitors). hCAT-1 protein abundance was estimated by Western blot, mRNA quantification by real time PCR, and SLC7A1 promoter activity by Luciferase activity (-1,606 and -650 bp promoter fragments from ATG). Specific protein 1 (Sp1), and total or phosphorylatedeNOSprotein was determined by Western blot. Sp1 activity (at four sites between -177 and -105 bp from ATG) was assayed by chromatin immunoprecipitation (ChIP) and vascular reactivity in umbilical vein rings. Insulin increased hCATs-L-arginine transport, maximal transport capacity (V-max/K-m), and hCAT-1 expression. NEM and L-lysine blocked L-arginine transport. In addition, it was trans-stimulated (similar to 7.8-fold) by L-lysine in absence of insulin, but unaltered (similar to 1.4-fold) in presence of insulin. Sp1 nuclear protein abundance and binding to DNA, and SLC7A1 promoter activity was increased by insulin. Insulin increasedNOsynthesis and caused endothelium-dependent vessel relaxation and reduced U46619-induced contraction, effects blocked by NEM and L-lysine, and dependent on extracellular L-arginine. We suggest that insulin induces human umbilical vein relaxation by increasing HUVEC L-arginine transport via hCATs (likely hCAT-1) most likely requiring Sp1-activated SLC7A1 expression. J. Cell. Physiol. 226: 2916-2924, 2011. (C) 2011 Wiley-Liss, Inc.
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    Una propuesta de revisión del método de caso.
    (1992) Gallardo, Victoria
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    The design of RutaCL Developing and measuring performance for highway typeface
    (2016) Gálvez Pizarro, Francisco; Gallardo, Victoria; Ramírez Montecinos, Rodrigo Fernando