Browsing by Author "Garcia-Diaz, D. F."

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    Chemically induced hypoxia promotes differential outcomes over preadipocyte- or adipocyte-macrophage communication
    (2017) Avila-George, K.; Ramos-Olivares, K.; Vasquez-Munoz, K.; Villanueva-Morales, V.; Reyes-Farias, M.; Quintero, P.; Garcia, L.; Garcia-Diaz, D. F.
    Expansion of white adipose tissue induce insufficient vascularization, driving hypoxia and low-grade inflammation. Resident preadipocytes are thought to be involved. We evaluated the effects of hypoxia over preadipocytes and adipocytes, to determine which cellular type impacts the most over macrophages activation. 3T3-L1 cells were either differentiated, or maintained undifferentiated. Each group was subjected to the presence or absence of chemical hypoxia (200 mu M CoCl2) for 24 h. Conditioned media were used as treatment for murine RAW264.7 macrophages for 24 h. Gene expression of HIF-1 alpha and TNF-alpha, and the release of several markers were assessed. It was observed that culture media from hypoxic preadipocytes induced greater expression of inflammatory markers and NO release than culture media from hypoxic adipocytes, by macrophages. Gene expression correlated closer with inflammatory markers release specially on macrophages treated with conditioned media from preadipocytes. Hence, the present work highlights the importance of preadipocytes on inflammatory conditions in vitro.
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    Maqui, Calafate, and Blueberry fruits extracts treatments suppress the pathogenic interaction amongst human adipocytes and macrophages
    (2020) Ovalle-Marin, A.; Reyes-Farias, M.; Vasquez, K.; Parra-Ruiz, C.; Quitral, V.; Jimenez, P.; Garcia, L.; Ramirez, L. A.; Quezada, J.; Gonzalez-Muniesa, P.; Garcia-Diaz, D. F.
    BACKGROUND: Obesity occurs due to a positive energy imbalance, leading to the expansion of adipose tissue. This phenomenon triggers a chronic low-grade inflammatory state, which is associated with comorbidities development. It is, therefore, of great interest to investigate new counteracting nutritional strategies. In this regard, polyphenol-rich Chilean native fruits, Aristotelia chilensis (Maqui) and Berberis microphylla (Calafate), and also the non-Chilean Vaccinium corymbosum (Blueberry), have been associated with antioxidant and anti-inflammatory features.
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    n-3 polyunsaturated fatty acids in the regulation of adipose tissue browning and thermogenesis in obesity: Potential relationship with gut microbiota
    (2021) Zapata, J.; Gallardo, A.; Romero, C.; Valenzuela, R.; Garcia-Diaz, D. F.; Duarte, L.; Bustamante, A.; Gasaly, N.; Gotteland, M.; Echeverría González, Francisca Cecilia
    Background: Obesity is a worldwide public health problem characterized by fat tissue accumulation, favouring adipose tissue and metabolic alterations. Increasing energy expenditure (EE) through brown adipose tissue activation and white adipose tissue (WAT) browning has gained relevance as a therapeutic approach. Different bioactive compounds, such as n-3 polyunsaturated fatty acids (PUFA), have been shown to induce those thermogenic effects. This process is regulated by the gut microbiota as well. Nevertheless, obesity is characterized by gut microbiota dysbiosis, which can be restored by weight loss and n-3 PUFA intake, among other factors. Knowledge gap: However, the role of the gut microbiota on the n-3 PUFA effect in inducing thermogenesis in obesity has not been fully elucidated. Objective: This review aims to elucidate the potential implications of this interrelation on WAT browning adiposw sittue (BAT), BAT activity, and EE regulation in obesity models.