Browsing by Author "Godoy, Liliana"

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    Antimicrobial Activity of Leaf Aqueous Extract of Schinus polygamus (Cav.) Cabrera against Pathogenic Bacteria and Spoilage Yeasts
    (2024) Acuna-Fontecilla, Andrea; Bruna, Julio; Ganga, Maria Angelica; Godoy, Liliana
    The antimicrobial activity of an aqueous extract of the leaves of Schinus polygamus (cav.) Cabrera against microorganisms of food importance was evaluated. First, the leaf aqueous extract of Schinus polygamus was characterized, quantifying hydroxycinnamic acids and phenolic compounds. Then, a battery of strains was tested, including Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 14028, and Listeria monocytogenes ATCC 13932. Also, we tested wine spoilage yeasts such as Brettanomyces bruxellensis LAMAP2480, B. bruxellensis LAMAP1359, B. bruxellensis CECT1451, and Pichia guilliermondii NPCC1051. Tests were conducted using the kinetic curve of growth and cell viability counts. The results indicate that with 10% v/v of concentrated extract, it is possible to observe growth inhibition of all microorganisms studied, with statistically significant differences during the whole measurement time (70 h for bacteria and 145 h for yeast).
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    Comparative proteome analysis of brettanomyces bruxellensis under hydroxycinnamic acid growth
    (2016) Carmona, Lourdes.; Godoy, Liliana; Varela, Javier.; Ganga, María Angélica.
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    Comparative transcriptome assembly and genome-guided profiling for Brettanomyces bruxellensis LAMAP2480 during p-coumaric acid stress
    (2016) Godoy, Liliana; Vera Wolf, Patricia.; Martínez, Claudio.; Ugalde, Juan A.; Ganga, María Angélica.
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    Comparison of the behaviour of brettanomyces bruxellensis strain LAMAP L2480 growing in authentic and synthetic wines
    (2015) Coronado, P.; Godoy, Liliana; Aguilera, S.; Carmona, Lourdes.; Martínez Fernández, Claudio.; Ganga, María Angélica.
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    Draft genome sequence and transcriptome analysis of the wine spoilage yeast dekkera bruxellensis LAMAP2480 provides insights into genetic diversity, metabolism and survival
    (2014) Valdés F., Jorge; Godoy, Liliana; Tapia, Paz.; Cepeda, Victoria.; Varela, Javier.; Cubillos Riffo, Francisco.; Silva Moreno, Evelyn.; Martínez Fernández, Claudio.; Ganga, María Angélica.
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    Effect of Light and p-Coumaric Acid on the Growth and Expression of Genes Related to Oxidative Stress in Brettanomyces bruxellensis LAMAP2480
    (2021) Catrileo, Daniela; Moreira, Sandra; Angelica Ganga, Maria; Godoy, Liliana
    Brettanomyces bruxellensis is considered the most significant contaminant yeast in the wine industry since it causes a deterioration in the organoleptic properties of the wine and significant economic losses. This deterioration is due to the production of volatile phenols from hydroxycinnamic acids. These compounds possess antimicrobial properties; however, B. bruxellensis can resist this effect because it metabolizes them into less toxic ones. Recent studies have reported that B. bruxellensis grows under different stress conditions, including p-coumaric acid (pCA) but effective methods for its control have not been found yet. Since that in other yeasts, such as Saccharomyces cerevisiae, it has been described that light affects its growth, and we evaluated whether the light would have a similar effect on B. bruxellensis. The results show that at light intensities of 2,500 and 4,000 lux in the absence of pCA, B. bruxellensis LAMAP2480 does not grow in the culture medium; however, when the medium contains this acid, the yeast adapts to both factors of stress managing to grow. The expression of genes related to oxidative stress in B. bruxellensis LAMAP2480, such as SOD1, GCN4, and ESBP6, showed a higher relative expression when the yeast was exposed to 2,500 lux compared to 4,000 lux, agreeing with the growth curves. This suggests that a higher expression of the genes studied would be related to stress-protective effects by pCA.
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    Encapsulación y evaluación de la cepa de Pichia kluyveri YCPUC83 en co-fermentaciones con S. cerevisiae EC1118 en mosto sintético
    (2023) Riquelme Toledo, Francisca Andrea; Godoy, Liliana; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
    Pichia kluyveri is a Non-Saccharomyces yeast that is characterized by its contribution to the aromatic potential of wines. However, it only survives in the first stage of fermentation before Saccharomyces cerevisiae completely dominates the medium. The main objective of this study was to evaluate the potential organoleptic contribution that Pichia kluyveri YCPUC83 has on the aromatic profile of wines obtained in co-fermentation with Saccharomyces cerevisiae EC1118. A sodium alginate-based medium that allows P. kluyveri to be encapsulated was evaluated in order to ensure its viability until the end of fermentation. Then, fermentations with encapsulated and non-encapsulated P. kluyveri were carried out in co-fermentation with S. cerevisiae EC1118 and oenological parameters and sensory profile of the wines were evaluated. The results of this study suggest that the encapsulation technique in P. kluyveri YCPUC83 turns out to be an interesting and innovative alternative for the oenological industry, since it is capable of improving fermentation performance, ensuring viability at the end of fermentation without affecting oenological parameters. On the other hand, co-fermenting with yeast improves the sensory profile of wines, as it demonstrates a clear trend in obtaining floral, fruity and tropical aromas compared to wines obtained by fermentation with S. cerevisiae EC1118.
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    Evaluation of antimicrobial activity from native wine yeast against food industry pathogenic microorganisms
    (2017) Acuña Fontecilla, Andrea.; Godoy, Liliana; Silva Moreno, Evelyn.; Ganga, María Angélica.
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    Genomics Perspectives on Metabolism, Survival Strategies, and Biotechnological Applications of Brettanomyces bruxellensis LAMAP2480
    (2017) Godoy, Liliana; Silva Moreno, E.; Mardones, W.; Guzmán Ángel, Daniela; Cubillos, F.A.; Ganga, María Angélica.
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    Identification of a second PAD1 in Brettanomyces bruxellensis LAMAP2480
    (2017) Godoy, Liliana; González, Camila.; Ganga, María Angélica.
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    Identification of the dekkera bruxellensis phenolic acid decarboxylase (PAD) gene responsible for wine spoilage
    (2014) Godoy, Liliana; García, Verónica.; Peña, Rubén.; Martínez Fernández, Claudio.; Ganga, María Angélica.
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    Non-conventional yeasts as biocontrol agents against fungal pathogens related to postharvest diseases
    (2022) Reyes-Bravo, Paula; Acuna-Fontecilla, Andrea; Marlene Rosales, Ines; Godoy, Liliana
    There are numerous investigations related to biological control and the use of microorganisms as new control strategies. In this sense, yeasts have been reported to have biocontrol activity and could be interesting candidates for BCA development. The main goal of this study was to evaluate the antagonist effect of four endophytic yeast, Naganishia antarctica YCPUC12, Aureobasidium pullulans YCPUC14, Cryptococcus terrestris YCPUC16 and Filobasidium oeirense YCPUC41 over the growth of Botrytis cinerea MPC40, Monilinia laxa MPC71, Penicillium expansum MPC102 and Geotrichum candidum MPC115.Three experimental approaches were used to evaluate biocontrol effect of yeast. Two in vitro assays (agar plug diffusion and dual-culture) and one in vivo assay. The results obtained here revealed that the four yeast strains evaluated showed inhibitory effect against the phytopathogens tested. Furthermore, it is indicated that the inhibitory effect by the yeasts over the fungi is differential, suggesting that there could be involved more than one antagonist-mechanism on the part of yeasts. Yeasts produce compounds capable of inhibiting the growth of fungi and, depending on the assay, the evaluated antagonist-yeasts have differential biocontrol effect against these pathogens. Their potential as biocontrol agents for postharvest diseases are interesting, and further investigation is needed to verify the effectiveness of these antagonists.
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    Non-ionizing electromagnetic fields for food safety
    (2018) Godoy, Liliana; Ganga, María Angélica.; B. Rodríguez, Arturo.
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    Purification and characterization of a p-coumarate decarboxylase and a vinylphenol reductase from Brettanomyces bruxellensis
    (2008) Godoy, Liliana; Garrido, D.; Martínez, Claudio.; Saavedra, J.; Combina, M.; Ganga, María Angélica.
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    Screening for indole-3-acetic acid synthesis and 1-aminocyclopropane-carboxylate deaminase activity in soil yeasts from Chile uncovers Solicoccozyma aeria as an effective plant growth promoter
    (2023) Carvajal Contardo, Mariajosé; Godoy, Liliana; Gebauer H., Marlene; Catrileo Aros, Daniela; Albornoz G., Francisco
    Background and aims: Plant growth-promoting microorganisms (PGPMs) stimulate plant growth by a series of mechanisms, including atmospheric nitrogen fixation, phosphorus solubilization and the synthesis of plant hormones. Within the group of PGPMs, several species of bacteria and fungi have been extensively studied. However, little information is available with regard to soil yeasts. The present study was conducted to identify yeast strains isolated from different soils in Chile that present plant growth promotion activity. Methods: Twenty-three strains were evaluated either for their capacity to synthesize indole-3-acetic acid or show aminocyclopropane-1-carboxylate deaminase activity. The effect on tomato seedling growth was evaluated in vitro, and two strains were selected for in vivo evaluation of plant growth and root ethylene synthesis. Results: All the strains analyzed presented IAA synthesis within the range between 0.8 and 3.3 ?g IAA mL?1. Solicoccozyma aeria (YCPUC75 and YCPUC79 strains) was the only yeast with ACC deaminase activity. In vitro inoculation of tomato seeds with eight of the evaluated strains resulted in an increase in the root volume and the number of lateral roots. In the second experiment, a 40% reduction in root ethylene synthesis was achieved by adding S. aeria (YCPUC79) to the root zone, which resulted in a 26% increase in plant growth. Conclusions: Solicoccozyma aeria YCPUC79 is an effective plant growth promoter stimulating root growth and reducing root ethylene synthesis.
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    Screening of native S. cerevisiae strains in the production of pajarete wine: a tradition of Atacama Region, Chile
    (2018) Lorca, Gustavo.; Godoy, Liliana; Uribe, Sandra.; Martínez Fernández, Claudio.; Ganga, María Angélica.
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    Screening of native Saccharomyces cerevisiae strains from Chile for beer production
    (2024) Moreira-Ramos, Sandra; Saavedra-Torrico, Jorge; G. Poblete, Camila; Godoy, Liliana; Sangorrin, Marcela; Ganga, María Angélica
    Introduction: Beer is one of the most consumed alcoholic drinks in the world, and this industry is a growing market that demands different properties to satisfy new consumers. The yeasts are used in different fermented beverages to contribute to new flavors. However, yeast strains used in the beer industry are limited so far, thus the diversity of flavors is very restricted. Therefore, the use of native yeast strains has been taking more importance with the purpose of conferring differentiated organoleptic properties to the product. Based on this observation the potentiality of native Saccharomyces cerevisiae strains obtained from different localities in Chile was researched. Methods: In this work was selected those strains that produced the highest ethanol concentration (nearly 6% v/v), consumed the highest amounts of sugars, and produced the lowest amounts of organic acids in the resulting beers. Finally, we did a beer tasting to select those strains that added different flavors to the final beer compared with a commercial strain used. Results and discussion: In this study, two native strains that produced fruity descriptors are described, which could be used in the future in brewing, craft or industrial production.
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    Selección de levaduras con capacidad de síntesis de ácido indolacético y/o actividad ACC desaminasa y su aplicación para promover el crecimiento vegetal
    (2022) Carvajal Contardo, Mariajosé; Godoy, Liliana; Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
    Las levaduras como promotores de crecimiento vegetal pueden mejorar la absorción de nutrientes mediante el aumento en el crecimiento radicular. El objetivo principal es promover el crecimiento vegetal en plántulas de tomate a través de la aplicación de levaduras con capacidad de síntesis de AIA y actividad ACC desaminasa. Para cuantificar el AIA, veintitrés cepas fueron cultivadas en medio YPD con y sin Triptófano, siguiendo el método colorimétrico de Salkowski. Para determinar la actividad ACCD las mismas cepas fueron cultivadas en el medio mínimo YNB con y sin ACC, se midió turbidez a los 14 días de incubación mediante DO y luego fue cuantificado como mM de alfa-cetobutirato. Luego, se evaluó el crecimiento radicular en tomates bajo el inoculo de las 23 cepas, se midió el número de raíces laterales, el largo de la raíz principal y el volumen radicular. Finalmente se cuantificó la producción de etileno en plantas de tomates inoculadas con dos cepas seleccionadas, obteniendo 4 tratamientos; Control, AIA: inóculo con la cepa con mayor producción de AIA, ACC: inóculo con cepa que tenga actividad ACCD y AIA/ACC: la mezcla de ambas cepas. Se midió etileno en la zona radicular. Todas las cepas fueron capaces de producir AIA con y sin Triptófano. Dos cepas de Soliococozyma aeria poseen actividad ACCD. El número de raíces laterales aumentó en la mayoría de los tratamientos con levaduras, mientras que el largo de la raíz principal disminuyó en todos los casos, excepto en el tratamiento con Rhodotorula babjevae. El tratamiento ACC disminuyó los niveles de etileno en las raíces, además obtuvo el mayor peso fresco aéreo y peso seco radicular, En conclusión, las cepas Metschnikowia sp. y S. aeria surgen como microorganismos promotores de crecimiento vegetal gracias a su producción de AIA y actividad ACCD.
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    Study of the coumarate decarboxylase and vinylphenol reductase activities of Dekkera bruxellensis (anamorph Brettanomyces bruxellensis) isolates
    (2009) Godoy, Liliana; Martínez, Claudio.; Carrasco, Nelson.; Ganga, María Angélica.