Browsing by Author "Henríquez Henríquez, Marcela Patricia"
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- ItemA thyroid genetic classifier correctly predicts benign nodules with Indeterminate cytology : two Independent, multicenter, prospective validation trials(2020) Zafereo, M.; McIver, B.; Vargas-Salas, S.; Domínguez Ruiz-Tagle, José Miguel; Steward, D. L.; Holsinger, F. C.; Kandil, E.; Williams, M.; Cruz, F.; Loyola, S.; Solar González, Antonieta Alejandra; Roa, J. C.; León, A.; Droppelman, N.; Lobos, M.; Arias, T.; Kong, C. T. S.; Busaidy, N.; Grubbs, E. G.; Graham, P.; Stewart, J.; Tang, AL; Wang, J.; Orloff, L.; Henríquez Henríquez, Marcela Patricia; Lagos, M.; Osorio, M.; Schachter, D.; Franco, C.; Medina, F.; Wohllk, N.; Díaz, R. E.; Véliz, J.; Horvath, E.; Tala, H.; Pineda, P.; Arroyo, P.; Vásquez, F.; Traipe, E.; Marin, L.; Miranda, G.; Bruce, E.; Bracamonte, M.; Mena, N.; González, H. E.
- ItemAnalytical and Clinical Validation for RT-qPCR Detection of SARS-CoV-2 Without RNA Extraction(2020) Miranda Marín, José Patricio; Henríquez Henríquez, Marcela Patricia; Osorio, Javiera; Videla, Mauricio; Ángel, Gladys; Camponovo, RossanaBackground: The recent COVID-19 pandemic has posed an unprecedented challenge to laboratory diagnosis, based on the amplification of SARS-CoV-2 RNA. With global contagion figures exceeding 4 million persons, the shortage of reagents for RNA extraction represents a bottleneck for testing globally. We present the validation results for an RT-qPCR protocol without prior RNA extraction. Due to its simplicity, this protocol is suitable for widespread application in resource-limited settings. Methods: Optimal direct protocol was selected by comparing RT-qPCR performance under a set of thermal (65, 70, and 95° for 5, 10, and 30 min) and amplification conditions (3 or 3.5 uL loading volume; 2 commercial RT-qPCR kits with a limit of detection below 10 copies/reaction) in nasopharyngeal swabs stored at 4°C in sterile Weise's buffer pH 7.2. The selected protocol was evaluated for classification concordance with a standard protocol (automated RNA extraction) in 130 routine samples and 50 historical samples with Cq values near to the clinical decision limit. Results: Optimal selected conditions for direct protocol were: thermal shock at 70°C for 10 min, loading 3.5 ul in the RT-qPCR. Prospective evaluation in 130 routine samples showed a 100% classification concordance with the standard protocol. The evaluation in historical samples, selected because their Cqs were at the clinical decision limit, showed 94% concordance with our confirmatory standard, which includes manual RNA extraction. Conclusions: Our results validate the use of this direct RT-qPCR protocol as a safe alternative for SARS-CoV-2 diagnosis in the case of a shortage of reagents for RNA extraction, with minimal clinical impact.
- ItemGenetic testing for indeterminate thyroid cytology: review and meta-analysis.(2018) Vargas Salas, Sergio; Martinez, Jose R.; Urra, Soledad; Domínguez Ruiz-Tagle, José Miguel; Mena, Natalia; Uslar, Thomas; Lagos Lucero, Marcela; Henríquez Henríquez, Marcela Patricia; González Díaz, Hernán
- ItemIntratask Variability As a Correlate for DRD4 and SLC6A3 Variants : A Pilot Study in ADHD(2015) Henríquez Henríquez, Marcela Patricia; Villarroel del Pino, Luis A.; Henríquez, Hugo; Zamorano, Francisco; Rothhammer, Francisco; Aboitiz, Francisco
- ItemLow serum sphingolipids in children with attention deficit-hyperactivity disorder(2015) Henríquez Henríquez, Marcela Patricia; Solari, Sandra; Quiroga, Teresa; Kim, Benjamin I.; Deckelbaum, Richard J.; Worgall, Tilla S.
- Itemω-3 Long-Chain Polyunsaturated Fatty Acids and Fatty Acid Desaturase Activity Ratios as Eventual Endophenotypes for ADHD(2015) Henríquez Henríquez, Marcela Patricia; Solari Gajardo, Sandra; Vargas, Gisela; Vasquez, Luis; Allende, Fidel; Castañón Santibáñez, Carla Alejandra; Tenorio Delgado, Marcela; Quiroga Gutiérrez, Sara Teresita