Browsing by Author "Krause, B. J."
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- ItemDynamic DNA methylation changes in early versus late adulthood suggest nondeterministic effects of childhood adversity : a meta-analysis(2020) Artigas, Rocío; Vega Tapia, F.; Hamilton, J.; Krause, B. J.
- ItemHypoxia-reduced nitric oxide synthase activity is partially explained by higher arginase-2 activity and cellular redistribution in human umbilical vein endothelium(W B SAUNDERS CO LTD, 2011) Prieto, C. P.; Krause, B. J.; Quezada, C.; San Martin, R.; Sobrevia, L.; Casanello, P.Hypoxia relates with altered placental vasodilation, and in isolated endothelial cells, it reduces activity of the endothelial nitric oxide synthase (eNOS) and L-arginine transport. It has been reported that arginase-2 expression, an alternative pathway for L-arginine metabolism, is increased in adult endothelial cells exposed to hypoxia as well as in pre-eclamptic placentae. We studied in human umbilical vein endothelial cells (HUVEC) whether hypoxia-reduced NO synthesis results from increased arginase-mediated L-arginine metabolism and changes in subcellular localization of eNOS and arginase-2. In HUVEC exposed (24 h) to 5% (normoxia) or 2% (hypoxia) oxygen, L-arginine transport kinetics, arginase activity (urea assay), and NO synthase (NOS) activity (L-citrulline assay) were determined. Arginase-1, arginase-2 and eNOS expression were determined by RT-PCR and Western blot. Subcellular localization of arginase-2 and eNOS were studied using confocal microscopy and indirect immunofluorescence. Experiments were done in absence or presence of S-(2-boronoethyl)-L-cysteine-HCl (BEC, arginase inhibitor) or N-G-nitro-L-arginine methyl ester (L-NAME). Hypoxia-induced reduction in eNOS activity was associated with a reduction in eNOS phosphorylation at Serine-1177 and increased phosphorylation at Threonine-495. This was paralleled with an induction in arginase-2 expression and activity, and decreased L-arginine transport. In hypoxia the arginase inhibition, restored NO synthesis and L-arginine transport, without changes in the eNOS post-translational modification status. Hypoxia increased arginase-2/eNOS colocalization, and eNOS redistribution to the cell periphery. Altogether these data reinforce the thought that eNOS cell location, post-translational modification and substrate availability are important mechanisms regulating eNOS activity. If these mechanisms occur in pregnancy diseases where feto-placental oxygen levels are reduced remains to be clarified. (C) 2011 Elsevier Ltd. All rights reserved.
- ItemLeptin in Cord Blood Associates with Asthma Risk at Age 3 in the Offspring of Women with Gestational Obesity(2020) Castro Rodríguez, José Antonio; Forno, E.; Casanello Toledo, Paola Cecilia; Padilla Pérez, Oslando; Krause, B. J.; Uauy, Ricardo
- ItemMaternal Obesity Is Associated With Higher Cord Blood Adipokines in Offspring Most Notably in Females.(2021) Jaramillo-Ospina, Á; Castaño-Moreno, E.; Muñoz-Muñoz, E.; Krause, B. J.; Uauy, R.; Casanello, P.; Castro-Rodríguez, José Antonio
- ItemPPARGC1A Gene Promoter Methylation as a Biomarker of Insulin Secretion and Sensitivity in Response to Glucose Challenges(2020) Santos Martín, José Luis; Krause, B. J.; Cataldo Bascuñan, Luis Rodrigo; Vega, Javier Andrés; Salas Pérez, F.; Mennickent, P.; Gallegos, R.; Milagro, F. I.; Prieto Hontoria, P.; Riezu-Boj, J. I.; Galgani Fuentes, José; Bravo, C.; Salas Huertos, A.; Arpon, A.; Martínez, J. A.
- ItemStim-activated TRPC-ORAI channels in pulmonary hypertension induced by chronic intermittent hypoxia(2020) Castillo Galán, Sebastián; Arenas Menéndez, German Alberto; Reyes, R. V.; Krause, B. J.; Iturriaga Agüera, Rodrigo