Browsing by Author "Martínez, J."
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- ItemA 10-Gene Classifier for Indeterminate Thyroid Nodules: Development and Multicenter Accuracy Study(2017) González Oneto, Humberto; Martínez, J.; Vargas Salas, Sergio; Solar González, Antonieta Alejandra; Véliz García, Loreto Pamela; Cruz, F.; Arias, T.; Loyola Zunino, Sandra; Horvath, E.; Tala, H.; Traipe, E.; Meneses, M.; Marín, L.; Wohllk, N.; Díaz, R.; Véliz, J.; Pineda, P.; Arroyo, P.; Mena, N.; Bracamonte, M.; Miranda, G.; Bruce, E.; Urra, S
- ItemA real-time PCR-based strategy for the detection of Paenibacillus larvae vegetative cells and spores to improve the diagnosis and the screening of American foulbrood(2010) Martínez, J.; González Ojeda, Bernardo
- ItemChitosan and platelet-derived growth factor synergistically stimulate cell proliferation in gingival fibroblasts(2013) Silva, Daniel; Arancibia, Rodrigo; Tapia, Cristián; Acuña Rougier, Cristina; Díaz Dosque, Mario; Cáceres, Mónica; Martínez, J.; Smith, Patricio C.
- ItemEffects of cigarette smoke and nicotine on cell viability, migration and myofibroblastic differentiation(2012) Silva, D.; Cáceres, M.; Arancibia, R.; Martínez, C.; Martínez, J.; Smith, P. C.
- ItemGastrointestinal neuroendocrine tumor with unresectable liver metastases: An example of multimodal therapeutic approach(2015) Martínez, J.; Besa, S.; Arab, J.P.; Quintana, J.C.; Regonesi, C.; Huete, A.; Jarufe, N.; Guerra, J.F.; Benítez, C.; Arrese, M.
- ItemMethylglyoxal and methylglyoxal-modified collagen as inducers of cellular injury in gingival connective tissue cells(2016) Retamal, I. N.; Hernández, R.; González-Rivas, C.; Cáceres, M.; Arancibia, R.; Romero, A.; Martínez, Constanza; Tobar, N.; Martínez, J.; Smith, P. C.Background and Objectives: Methylglyoxal is a toxic product derived from glucose metabolism that plays a role in inflammation, diabetes and aging. In addition, the periodontal pathogen Tannerella forsythensis may also generate this compound. However, the effects of methylglyoxal on gingival cells are still poorly understood. In the present study, we have explored whether methylglyoxal or methylglyoxal-treated collagen may modulate cell viability, death and proliferation in gingival connective tissue cells. In addition, we have searched for inflammatory mediators secreted by cells upon exposure to these conditions. Material and Methods: Primary cultures of human gingival fibroblasts were stimulated with soluble methylglyoxal or cultured over a collagen matrix glycated by this agent. Cell viability was evaluated through the MTS assay. Cell death was assessed through DAPI nuclear staining, annexin V and propidium iodide assays. Cell proliferation was evaluated through double immunofluorescence for DAPI and Ki67. Protein levels of matrix metalloproteinases and cytokines were assessed through antibody arrays, enzyme-linked immunosorbent assay, real-time reverse transcription polymerase chain reaction and immunofluorescence. Statistical analysis was performed using the Kruskall-Wallis and Mann-Whitney tests. Results: Soluble methylglyoxal, but not culture of gingival fibroblasts over a methylglyoxal-modified collagen matrix, induced a reduction on cell viability. Moreover, soluble methylglyoxal induced apoptotic cell death as indicated by DAPI nuclear staining, annexin V and propidium iodide assays. Neither soluble methylglyoxal, nor methylglyoxal-modified collagen modified cell proliferation. Using an antibody array, enzyme-linked immunosorbent assay and immunofluorescence assays, we determined that both, soluble methylglyoxal and methylglyoxal- modified collagen stimulated an increase in tissue inhibitor of metalloproteinase (TIMP)-1 protein levels. Conclusions: Soluble methylglyoxal is a highly cytotoxic compound that induces cell death through apoptosis in gingival fibroblasts. TIMP-1 is induced in these cells upon direct exposure to methylglyoxal or after culture of gingival fibroblasts over methylglyoxal-treated collagen. As TIMP-1 has been implicated in cell survival and matrix remodeling, we propose that increased TIMP-1 protein levels may be part of a protective response of gingival connective tissue cells upon exposure to methylglyoxal or after the interaction with the collagen matrix that has been modified by this agent.
- ItemNeoadjuvant chemoradiation therapy for borderline pancreatic adenocarcinoma: report of two cases(2013) Galindo, J.; Gabrielli Nervi, Mauricio; Guerra, J. F.; Cassina, J. C.; Garrido, M.; Jarufe Cassis, Nicolás; Borghero Ríos, Yerko Orestes; Madrid Arenas, Jorge; Zoroquiain Vélez, José Pablo; Roa Strauch, Juan Carlos Enrique; Martínez, J.
- ItemPortal Angiogenesis in Chronic Liver Disease Patients Correlates with Portal Pressure and Collateral Formation(2019) Serrano Honeyman, Carolina; Ling, S. C.; Verdaguer, S.; León, M.; Jarufe Cassis, Nicolás; Guerra, J. F.; Pattillo Silva, Juan Carlos; Benítez, C.; Villagrán, A.; Torres, J.; Concha, M.; Villarroel del Pino, Luis A.; Dellepiane, P.; Domínguez, P.; Martínez, J.; Gana Ansaldo, Juan Cristóbal
- ItemProbing 3D structure with a large MUSE mosaic : extending the mass model of Frontier Field Abell 370(2019) Lagattuta, D. J.; Richard, J.; Bauer, Franz Erik; Clement, B.; Mahler, G.; Soucail, G.; Carton, D.; Kneib, J. P.; Laporte, Nicolás; Martínez, J.; Patricio, V.; Payne, A. V.; Pello, R.; Schmidt, K. B.; De La Vieuville, G.
- ItemQSAR model for ethylene polymerisation catalysed by supported bis(imino)pyridine iron complexes(2007) Cruz, V. L.; Martínez, J.; Martínez Salazar, J.; Ramos, J.; Reyes, M. L.; Toro Labbé, Alejandro; Gutiérrez Oliva, Soledad
- ItemSignificant transfer of surgical skills obtained with an advanced laparoscopic training program to a laparoscopic jejunojejunostomy in a live porcine model: Feasibility of learning advanced laparoscopy in a general surgery residency(2012) Varas Cohen, Julian Emanuel; Mejía, R.; Riquelme, A.; Maluenda, F.; Buckel, E.; Salinas, J.; Martínez, J.; Aggarwal, R.; Jarufe, N.; Boza, C.
- ItemSoluble MMP-14 produced by bone marrow-derived stromal cells sheds epithelial endoglin modulating the migratory properties of human breast cancer cells(2014) Tobar, N.; Avalos M., Celeste; Mendez, N; Smith, Patricio C.; Bernabeu, C.; Quintanilla, M.; Martínez, J.
- ItemThe delay of shock breakout due to circumstellar material evident in most type II supernovae(2018) Forster, F.; Moriya, T. J.; Maureira, J. C.; Anderson, J. P.; Blinnikov, S.; Bufano, F.; Cabrera Vives, G.; Clocchiatti, Alejandro; De Jaeger, T.; Estevez, P. A.; Galbany, L.; González -Gaitán, S.; Grafener, G.; Hamuy, M.; Hsiao, E. Y.; Huentelemu, P.; Huijse, P.; Kuncarayakti, H.; Martínez, J.; Medina, G.; Olivares, F.; Pignata, Giuliano; Razza, A.; Reyes, I.; San Martín, J.; Smith, R. C.; Vera, E.; Vivas, A. K.; Postigo, A. D.; Yoon, S. C.; Ashall, C.; Fraser, M.; Gal-Yam, A.; Kankare, E.; Le Guillou, L.; Mazzali, P. A.; Walton, N. A.; Young, D. R.
- ItemThe reaction force. A scalar property to characterize reaction mechanisms(2009) Martínez, J.; Toro Labbé, Alejandro
- ItemTrasplante hepático en pacientes con trombosis de la vena porta(2013) Gabrielli Nervi, Mauricio; Galindo, J.; Figueroa, E.; Moisan, F.; Arrese Jiménez, Marco; Benítez, Carlos; Soza, Alejandro; Dominguez, P.; Pérez Ayuso, Rosa María; Guerra Castro, Juan Francisco; Jarufe Cassis, Nicolás; Martínez, J.
- ItemTumor Necrosis Factor-alpha Inhibits Transforming Growth Factor-beta-Stimulated Myofibroblastic Differentiation and Extracellular Matrix Production in Human Gingival Fibroblasts(2013) Arancibia, R.; Oyarzún, A.; Silva, D.; Tobar, N.; Martínez, J.; Smith, Patricio C.
- ItemUncoupled inflammatory, proliferative, and cytoskeletal responses in senescent human gingival fibroblasts(2020) Páez, J.; Hernández Salinas, Romina; Rojas Cortéz, Leticia Andrea; Espinoza, J.; Martínez, Constanza; Tobar, N.; Martínez, J.; Smith, P. C.