Browsing by Author "Navarrete, Jose"

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    Deterioration, decay and identification of fungi isolated from wooden structures at the Humberstone and Santa Laura saltpeter works: A world heritage site in Chile
    (ELSEVIER SCI LTD, 2014) Ortiz, Rodrigo; Navarrete, Hugo; Navarrete, Jose; Parraga, Mario; Carrasco, Ivo; de la Vega, Eduardo; Ortiz, Manuel; Herrera, Paula; Blanchette, Robert A.
    The use of wood in construction has been part of mankind's history but wood placed into the environment is affected by biotic and abiotic agents and is degraded over time. Even in extreme environments, such as dry desert sites, deterioration of wood can take place. One site located in the Atacama Desert in northern Chile is the Humberstone and Santa Laura saltpeter works where offices and other structures were built of wood. Founded in 1872, the Humberstone and Santa Laura Saltpeter Works was designated a UNESCO World Heritage Site in 2005 for its historic significance. Since significant deterioration in the wooden buildings has taken place, investigations were initiated to better understand the degradation underway so conservation efforts to protect the historic buildings can be developed. The objectives of this study were to identify the type of deterioration and decay taking place and to isolate and identify fungi from wood samples of structural elements at both sites. Samples of deteriorated wood showed extensive degradation that resulted in a defibration of the wood. The middle lamella between cells was degraded and remaining secondary walls separated due to high concentrations of salts. This resulted in a serious corrosion of the exterior layers of wood cells. Although high salts inhibit fungi, many different fungi were isolated. Sequencing of the ITS region of the rDNA was used and fungi were identified as Penicillium chrysogenum, Engyodontium album, Eupenicillium tropicum, Penicillium digitatum, Pseudotaeniolina globosa, Cladosporium phaenocomae, Aureobasidium pullulans, Penicillium virgatum, Coprinopsis sp. and Phanerochaete sordida. Several of these fungi appear to be halophilic. (C) 2013 Elsevier Ltd. All rights reserved.
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    Investigations of Biodeterioration by Fungi in Historic Wooden Churches of Chilo,, Chile
    (SPRINGER, 2014) Ortiz, Rodrigo; Parraga, Mario; Navarrete, Jose; Carrasco, Ivo; de la Vega, Eduardo; Ortiz, Manuel; Herrera, Paula; Jurgens, Joel A.; Held, Benjamin W.; Blanchette, Robert A.
    The use of wood in construction has had a long history and Chile has a rich cultural heritage of using native woods for building churches and other important structures. In 2000, UNESCO designated a number of the historic churches of Chilo,, built entirely of native woods, as World Heritage Sites. These unique churches were built in the late 1700 s and throughout the 1800 s, and because of their age and exposure to the environment, they have been found to have serious deterioration problems. Efforts are underway to better understand these decay processes and to carryout conservation efforts for the long-term preservation of these important structures. This study characterized the types of degradation taking place and identified the wood decay fungi obtained from eight historic churches in Chilo,, seven of them designated as UNESCO World Heritage sites. Micromorphological observations identified white, brown and soft rot in the structural woods and isolations provided pure cultures of fungi that were identified by sequencing of the internal transcribed region of rDNA. Twenty-nine Basidiomycota and 18 Ascomycota were found. These diverse groups of fungi represent several genera and species not previously reported from Chile and demonstrates a varied microflora is causing decay in these historic buildings.