Browsing by Author "Paredes, L."

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    A new multiplex PCR assay for the simultaneous detection of vancomycin-resistant enterococci from rectal swabs
    (2010) Benadof, D.; San Martín, M.; Aguirre, J.; Paredes, L.; Malig, R.; Melo Ledermann, Francisco Javier; Lehouque, G.
    Objectives This study describes the diagnostic performance of a recently available multiplex PCR-based kit for the simultaneous detection and identification of Enterococcus faecium, Enterococcus faecalis, vanA, vanB, vanC1 and vanC2/C3 genes, directly from rectal swabs constituting the most complete existing molecular assay currently available. Methods The diagnostic performance of this assay was evaluated by a multicenter study involving three independent public hospitals and consisted in the analysis of 187 rectal swabs from patients at high risk for vancomycin-resistant enterococci colonization. Results When bacteria culture was used as the gold standard, the sensitivity, specificity, positive and negative predicted values for the assay were 96.8%, 76.0%, 67.7% and 97.9%, respectively. When a composite reference standard consisting of culture and DNA sequencing of PCR products was used as the gold standard, the sensitivity, specificity, positive and negative predicted values for the PCR-based assay were 97.8%, 96.9%, 96.7% and 97.9%, respectively. Conclusions Based on these results, we conclude that this assay is considerably more sensitive than traditional microbiological methods for detecting vancomycin-resistant enterococci from rectal swabs. It is also much faster than culture. We believe that the implementation of this assay in routine clinical laboratories could help to reduce hospital-acquired vancomycin-resistant enterococci infections.
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    MASCARA-4 b/bRing-1b - A retrograde hot Jupiter around the bright A3V star HD 85628
    (2019) Brahm García, Enrique; Zapata, Abner; Jordán Colzani, Andrés Cristóbal; Vanzi, Leonardo; Dorval, P.; Talens, G.J.J.; Otten, G.P.P.L.; Henry, T.; Paredes, L.; Jao, W.C.; James, H.