Browsing by Author "Quezada, C."
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- ItemAdenosine A(2B) receptor mediates an increase on VEGF-A production in rat kidney glomeruli(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2008) Valladares, D.; Quezada, C.; Montecinos, P.; Yanez, Aj.; Sobrevia, L.; Martin, R. SanUp-regulation of the glomerular expression and the activity of vascular endothelial growth factor-A (VEGF) have been identified as an early pathogenic event for the progression of diabetic nephropathy. Currently, however the mediators are not yet clearly recognized. In this study we identified all four adenosine receptor (AR) subtypes, i.e. A(1), A(2A), A(2B) and A(3) in isolated rat kidney glomeruli. We localized the expression of A(2B)AR in podocytes, the primary VEGF producing cells. The ex vivo treatment of kidney glomeruli with adenosine or a general AR agonist NECA, increases VEGF protein content. In addition, NECA treatment elicits VEGF release. These effects were blocked by the A(2B)AR selective antagonist MRS1754 supplementation. Furthermore, we showed that A(2B)AR activation was necessary to promote a higher expression of VEGF in kidney glomeruli upon exposure to high D-glucose concentration, a pathogenic condition like those observed in diabetic nephropathy. (c) 2007 Elsevier Inc. All rights reserved.
- ItemAdenosine mediates transforming growth factor-beta 1 release in kidney glomeruli of diabetic rats(WILEY, 2009) Roa, H.; Gajardo, C.; Troncoso, E.; Fuentealba, V.; Escudero, C.; Yanez, A.; Sobrevia, L.; Pastor Anglada, M.; Quezada, C.; San Martin, R.Up regulation of the transforming growth factor-beta 1 (TGF-beta 1) axis has been recognized as a pathogenic event for progression of glomerulosclerosis in diabetic nephropathy. We demonstrate that glomeruli isolated from diabetic rats accumulate up to sixfold more extracellular adenosine than normal rats. Both decreased nucleoside uptake activity by the equilibrative nucleoside transporter 1 and increased AMP hydrolysis contribute to raise extracellular adenosine. Ex vivo assays indicate that activation of the low affinity adenosine A(2B) receptor subtype (A(2B)AR) mediates TGF-beta 1 release from glomeruli of diabetic rats, a pathogenic event that could support progression of glomerulopathy when the bioavailability of adenosine is increased. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
- ItemAkt/mTOR Role in Human Foetoplacental Vascular Insulin Resistance in Diseases of Pregnancy(2017) Villalobos Labra, Roberto Esteban; Silva L; Subiabre Morales, Mario Enrique; Araos J; Salsoso Rodríguez, M. Rocío; Fuenzalida Saavedra, Bárbara; Sáez, Tamara; Toledo F; Leiva Mendoza, Andrea Alejandra; Sobrevía Luarte, Luis Alberto; Gonzalez, M.; Quezada, C.; Pardo, F.; Chiarello, Di
- ItemComparing bulge RR Lyrae stars with bulge giants: Insight from 3D kinematics(2024) Carvajal, J. Olivares; Zoccali, M.; De Leo, M.; Ramos, R. Contreras; Quezada, C.; Rojas-Arriagada, A.; Valenti, E.; Albarracin, R.; Navarro, A. ValenzuelaContext. The structure and kinematics of the old component of the Galactic bulge are still a matter of debate. It is clear that the bulk of the bulge as traced by red clump stars includes two main components, which are usually identified as the metal-rich and metal-poor components. They have different shapes, kinematics, mean metallicities, and alpha-element abundances. It is our current understanding that they are associated with a bar and a spheroid, respectively. On the other hand, RR Lyrae variables trace the oldest population of the bulge. While it would be natural to think that they follow the structure and kinematics of the metal-poor component, the data analysed in the literature show conflicting results. Aims. We aim to derive a rotation curve for bulge RR Lyrae stars in order to determine that the old component traced by these stars is distinct from the two main components observed in the Galactic bulge. Methods. This paper combines APOGEE-2S spectra with OGLE-IV light curves, near-infrared photometry, and proper motions from the VISTA Variables in the V & iacute;a L & aacute;ctea survey for 4193 RR Lyrae stars. Six-dimensional phase-space coordinates were used to calculate orbits within an updated Galactic potential and to isolate the stars. Results. The stars that stay confined within the bulge represent 57% of our sample. Our results show that bulge RR Lyrae variables rotate more slowly than metal-rich red clump stars and have a lower velocity dispersion. Their kinematics is compatible with them being the low-metallicity tail of the metal-poor component. We confirm that a rather large fraction of halo and thick disc RR Lyrae stars pass by the bulge within their orbits, increasing the velocity dispersion. A proper orbital analysis is therefore critical to isolate bona fide bulge variables. Finally, bulge RR Lyrae seem to trace a spheroidal component, although the current data do now allow us to reach a firm conclusion about the spatial distribution.
- ItemDesarrollo de la granulación durante la maduración de naranjas de ombligo tardías en la Zona Central de Chile(2000) Ortúzar, Juan E.; Barrales V., Luis; Peña Neira, Alvaro Iván; Carmona Morales, Patricia; Valdivieso Gatica, Gonzalo; Quezada, C.
- ItemHypoxia-reduced nitric oxide synthase activity is partially explained by higher arginase-2 activity and cellular redistribution in human umbilical vein endothelium(W B SAUNDERS CO LTD, 2011) Prieto, C. P.; Krause, B. J.; Quezada, C.; San Martin, R.; Sobrevia, L.; Casanello, P.Hypoxia relates with altered placental vasodilation, and in isolated endothelial cells, it reduces activity of the endothelial nitric oxide synthase (eNOS) and L-arginine transport. It has been reported that arginase-2 expression, an alternative pathway for L-arginine metabolism, is increased in adult endothelial cells exposed to hypoxia as well as in pre-eclamptic placentae. We studied in human umbilical vein endothelial cells (HUVEC) whether hypoxia-reduced NO synthesis results from increased arginase-mediated L-arginine metabolism and changes in subcellular localization of eNOS and arginase-2. In HUVEC exposed (24 h) to 5% (normoxia) or 2% (hypoxia) oxygen, L-arginine transport kinetics, arginase activity (urea assay), and NO synthase (NOS) activity (L-citrulline assay) were determined. Arginase-1, arginase-2 and eNOS expression were determined by RT-PCR and Western blot. Subcellular localization of arginase-2 and eNOS were studied using confocal microscopy and indirect immunofluorescence. Experiments were done in absence or presence of S-(2-boronoethyl)-L-cysteine-HCl (BEC, arginase inhibitor) or N-G-nitro-L-arginine methyl ester (L-NAME). Hypoxia-induced reduction in eNOS activity was associated with a reduction in eNOS phosphorylation at Serine-1177 and increased phosphorylation at Threonine-495. This was paralleled with an induction in arginase-2 expression and activity, and decreased L-arginine transport. In hypoxia the arginase inhibition, restored NO synthesis and L-arginine transport, without changes in the eNOS post-translational modification status. Hypoxia increased arginase-2/eNOS colocalization, and eNOS redistribution to the cell periphery. Altogether these data reinforce the thought that eNOS cell location, post-translational modification and substrate availability are important mechanisms regulating eNOS activity. If these mechanisms occur in pregnancy diseases where feto-placental oxygen levels are reduced remains to be clarified. (C) 2011 Elsevier Ltd. All rights reserved.
- ItemInterfacial behavior of PAMAM-PCL dendrimers and in situ spontaneous formation of gold nanoparticles at the toluene-water and air-water interfaces: Experimental and theoretical studies(2016) Saldías, César; Méndez Lesser, Manuel; Saavedra, M.; Pereira, A.; Rojas, M.; Avila, F.; Bonardd Salvador, Sebastián Ignacio; Pino, M.; Saldías, S.; Quezada, C.; Leiva, A.; Radić Foschino, Deodato D.
- ItemMultidrug resistance in glioblastoma stem-like cells: Role of the hypoxic microenvironment and adenosine signaling(2017) Uribe, D.; Torres, A.; Rocha, J.; Niechi, I.; Oyarzun, C.; Sobrevía Luarte, Luis Alberto; San Martin, R.; Quezada, C.
- ItemNueva normalidad, vieja precariedad : la crisis pandémica en Santiago de Chile(2020) Di Giminiani, Piergiorgio; Pérez, M.; Quezada, C.
- ItemVVV catalog of ab-type RR Lyrae in the inner Galactic bulge(2024) Zoccali, Manuela; Quezada, C.; Contreras Ramos, R.; Valenti, E.; Valenzuela-Navarro, A.; Olivares Carvajal, J.; Rojas Arriagada, A.; Minniti, J.H.; Gran, F.; De Leo, M.Context. Observational evidence has accumulated in recent years, showing that the Galactic bulge includes two populations, a metal-poor one and a metal-rich one, which in addition to having different metallicities show different alpha over iron abundances, spatial distribution, and kinematics. While the metal-rich, barred component has been fairly well characterized, the metal-poor, spheroidal component has been more elusive and harder to describe. RR Lyrae variables are clean tracers of the old bulge component, and they are, on average, more metal-poor than red clump stars. Aims. In the present paper, we provide a new catalog of 16488 ab-type RR Lyrae variables in the bulge region within |l|≲10° and |b|≲2.8°, extracted from multi-epoch Point Spread Function photometry performed on VISTA Variable in the Vía Láctea survey data. We used the catalog to constrain the shape of the old, metal-poor, bulge stellar population. Methods. The identification of ab-type RR Lyrae among a large sample of candidate variables of different types has been performed via a combination of a Random Forest classifier and visual inspection. We optimized this process in such a way to extract a clean catalog with high purity, although for this reason its completeness, close to the midplane, is lower compared to a few other near-infrared catalogs covering the same region of the sky. Results. We used the present catalog to derive the shape of their distribution around the Galactic center, resulting in an elongated spheroid with projected axis ratio of b/a∼0.7 and an inclination angle of φ∼20 degrees. We discuss how observational biases, such as errors on the distances and a nonuniform sampling in longitude, affect both the present measurements and previous ones, especially those based on red clump stars. Because the latter have not been taken into account before, we refrain from a quantitative comparison between these shape parameters and those derived for the main Galactic bar. Nonetheless, qualitatively, taking into account observational biases would lower the estimated ellipticity of the bar derived from red clump stars, and hence reduce the difference with the present results. Conclusions. We publish a high-purity RRab sample for future studies of the oldest Galactic bulge population, close to the midplane. We explore different choices for the period-luminosity-metallicity relation, highlighting how some of them introduce spurious trends of distance with either the period or the metallicity, or both. We provide evidence that they trace a structure that is less elongated than the main bar, though we also highlight some biases of these kind of studies not discussed before.