Browsing by Author "Quezada, N"

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    Antioxidant activity of crude extract, alkaloid fraction, and flavonoid fraction from boldo (Peumus boldus Molina) leaves
    (2004) Quezada, N; Asencio, M; del Valle, JM; Aguilera, JM; Gómez, B
    Boldo is an endemic plant from Chile widely used in folk medicine, whose biological (mainly antioxidant) activity has been attributed to the presence of the alkaloid boldine. The aim of this study was to determine the contribution of the flavonoid and other crude fractions from boldo leaves to the antioxidant activity of extracts. The main constituent in the flavonoid fraction was also isolated. The antioxidant activity and reducing power of crude boldo extract and fractions were measured and compared with natural and synthetic antioxidants. The antioxidant activity of boldo leaf extracts came mainly from the flavonoid fraction (44.1%) followed by the alkaloid fraction (15.6%), with catechin and boldine being the main contributors of the antioxidant activity of these 2 fractions (60.9% and 35.6% of the total activity, respectively). On the basis of these results, we propose to use catechin and boldline as markers for the antioxidant activity of boldo leaves. Tailor-made antioxidants to be used in foods can be prepared by enriching these 2 fractions.
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    Solubility of boldo leaf antioxidant components (Boldine) in high-pressure carbon dioxide
    (ELSEVIER, 2005) de la Fuente, JC; Quezada, N; del Valle, JM
    This work contributes to the development of an enrichment process for antioxidant compounds in aqueous alcoholic extracts of boldo (Peumus boldus M.) leaves by using high-pressure CO2 as the solvent. Specifically we measured the high-pressure solubility (y(2), molar fraction) of a selected bioactive compound in boldo leaves (boldine) in CO, as a function of system temperature (298 K <= T <= 333 K) and pressure (8 MPa <= P <= 40 MPa). Experimental data was correlated by using a density-based model which is valid for solvent densities > 607 kg/m(3). Predicted solubility values are low (4 x 10(-7) <= y(2) <= 6 x 10(-5)) but comparable with those of nitrogen-containing organic compounds with similar molecular weight (327.4 Da) and solubility parameter (28.3 MPa (0.5) at 313 K) as boldine. (c) 2005 Elsevier B.V. All rights reserved.