Browsing by Author "Rodriguez, A"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Sexual dimorphism in number and proportion of neurons in the human median raphe nucleus
    (ELSEVIER, 2000) Cordero, ME; Valenzuela, CY; Torres, R; Rodriguez, A
    The number and proportion of neurons in the median raphe nuclei stained by the Golgy-Cox and Nissl methods was compared in males and females infants. When subjects are matched by age and cause of death the number and proportion of fusiform, ovoid and multipolar cells differs significantly between sexes at different ages. (C) 2000 Elsevier Science B.V. All rights reserved.
  • Thumbnail Image
    Item
    Sterol carrier protein 2 gene transfer changes lipid metabolism and enterohepatic sterol circulation in mice
    (W B SAUNDERS CO, 2000) Zanlungo, S; Amigo, L; Mendoza, H; Miquel, JF; Vio, C; Glick, JM; Rodriguez, A; Kozarsky, K; Quinones, V; Rigotti, A; Nervi, F
    Background & Aims: Sterol carrier protein 2 (SCP-2) enhances sterol cycling and facilitates cholesterol translocation between intracellular organelles and plasma membrane in cultured cells, including hepatocytes. We examined the role of SCP-2 in hepatic cholesterol and lipid trafficking through the sinusoidal and canalicular secretory pathways of the liver in vivo. Methods: Recombinant adenovirus-mediated SCP-2 gene transfer was used to obtain hepatic overexpression of SCP-2 in C57BL/6 mice. Results: SCP-2 overexpression in the mouse liver resulted in an 8-fold increase of SCP-2 protein levels and determined various effects on lipid metabolism. It decreased high-density lipoprotein cholesterol and increased low-density lipoprotein (LDL) cholesterol concentrations. The expressions of hepatic LDL receptor, apolipoprotein (apo) A-I, apoB, and apoE were decreased. SCP-2 overexpression also increased hepatic cholesterol concentration, associated with decreased cholesterol neosynthesis. Increased biliary cholesterol and bile acid secretion, bile acid pool size, and intestinal cholesterol absorption were also observed. Conclusions: These results indicate that modulation of SCP-2 expression in the liver determines important modifications on lipoprotein metabolism, hepatic cholesterol synthesis and storage, biliary lipid secretion, bile acid metabolism and intestinal cholesterol absorption.