Browsing by Author "Rodriguez-Cordova, Leonardo"

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    Bacterial networks in Atlantic salmon with Piscirickettsiosis
    (Springer Nature, 2023) Coca Rives, Yoandy; Godoy, Marcos; Pontigo, Juan Pablo; Caro, Diego; Maracaja-Coutinho, Vinicius; Arias-Carrasco, Raul; Rodriguez-Cordova, Leonardo; de Oca, Marco Montes; Saez-Navarrete, Cesar; Burbulis, Ian
    An unbalanced composition of gut microbiota in fish is hypothesized to play a role in promoting bacterial infections, but the synergistic or antagonistic interactions between bacterial groups in relation to fish health are not well understood. We report that pathogenic species in the Piscirickettsia, Aeromonas, Renibacterium and Tenacibaculum genera were all detected in the digesta and gut mucosa of healthy Atlantic salmon without clinical signs of disease. Although Piscirickettsia salmonis (and other pathogens) occurred in greater frequencies of fish with clinical Salmonid Rickettsial Septicemia (SRS), the relative abundance was about the same as that observed in healthy fish. Remarkably, the SRS-positive fish presented with a generalized mid-gut dysbiosis and positive growth associations between Piscirickettsiaceae and members of other taxonomic families containing known pathogens. The reconstruction of metabolic phenotypes based on the bacterial networks detected in the gut and mucosa indicated the synthesis of Gram-negative virulence factors such as colanic acid and O-antigen were over-represented in SRS positive fish. This evidence indicates that cooperative interactions between organisms of different taxonomic families within localized bacterial networks might promote an opportunity for P. salmonis to cause clinical SRS in the farm environment.
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    Salmo salar Skin and Gill Microbiome during Piscirickettsia salmonis Infection
    (2024) Godoy, Marcos; Coca, Yoandy; Suarez, Rudy; Montes de Oca, Marco; Bledsoe, Jacob W.; Burbulis, Ian; Caro, Diego; Pontigo, Juan Pablo; Maracaja-Coutinho, Vinicius; Arias-Carrasco, Raul; Rodriguez-Cordova, Leonardo; Saez-Navarrete, Cesar
    Maintaining the high overall health of farmed animals is a central tenant of their well-being and care. Intense animal crowding in aquaculture promotes animal morbidity especially in the absence of straightforward methods for monitoring their health. Here, we used bacterial 16S ribosomal RNA gene sequencing to measure bacterial population dynamics during P. salmonis infection. We observed a complex bacterial community consisting of a previously undescribed core pathobiome. Notably, we detected Aliivibrio wodanis and Tenacibaculum dicentrarchi on the skin ulcers of salmon infected with P. salmonis, while Vibrio spp. were enriched on infected gills. The prevalence of these co-occurring networks indicated that coinfection with other pathogens may enhance P. salmonis pathogenicity.