Browsing by Author "Sandoval, Felipe"

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    Periprosthetic Hip Fracture due to Ballistic Injuries
    (2024) Sandoval, Felipe; Valenzuela, Joaquin; Carmona, Maximiliano; Guiloff, Benjamin; Salgado, Martin
    Periprosthetic hip fractures are a common cause for revision. To date, however, there are no reports of periprosthetic fractures (PFs) in total hip arthroplasty caused by ballistic injury (BI). There are no current recommendations on the management of this pathology in the literature. The objective of this paper is to report on 2 successfully treated cases of PF caused by BIs. Additionally, a brief review of the literature regarding open fracture secondary to BIs is carried out. What we consider appropriate initial and definitive management for these patients is outlined. According to our clinical results and current evidence, adequate management for a BI Vancouver B1 femoral PF consists of early antibiotic therapy, surgical debridement, osteosynthesis with variable angle locking plate, structural allograft, cerclage wires, and negative pressure wound therapy. (c) 2024 The Authors. Published by Elsevier Inc. on behalf of The American Association of Hip and Knee Surgeons. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/).
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    Royal Jelly Derived Extracellular Vesicles Modulate Microglial Nanomechanics and Inflammatory Responses
    (2025) Zavala, Gabriela; Berríos, Pablo; Sandoval, Felipe; Bravo, Graciela; Barrera, Nelson P.; Alarcón Moyano, Jéssica; Díaz Calderón, Paulo; Aguayo, Sebastián; Schuh, Christina
    BACKGROUND Microglia, the braińs resident immune cells, undergo profound mechanical and functional changes upon activation contributing to neuroinflammation, a pathological signature of many neurological diseases. Thus, new anti-inflammatory treatment options are needed that tackle these mechanobiological alterations in microglia, which remain strongly understudied. In this context, extracellular vesicles (EVs) are crucial mediators of intercellular and interkingdom communication, yet their influence on the mechanobiological properties of recipient cells remains largely unknown. Honeybee-derived Royal Jelly EVs (RJEVs) have demonstrated remarkable anti-inflammatory properties, but their impact on microglial cellular nanomechanics and uptake mechanisms remains unclear. RESULTS In this study, we used a multi-disciplinary approach to analyze the resulting biological and nanomechanical changes following the activation of human microglia and the potential effect of RJEV treatment on these mechanobiological parameters. We observed that LPS treatment was associated with decreased cellular Young’s modulus, increased membrane fluidity, and enhanced motility of microglia, indicating a more migratory and pro-inflammatory phenotype. Additionally, LPS exposure altered cellular EV uptake mechanisms by shifting preference from an equilibrium of four mechanisms to the predominance of macropinocytosis and clathrin-dependent endocytosis. Remarkably, RJEV treatment counteracted these mechanobiological changes by, in turn, increasing microglial stiffness, reducing motility, and decreasing secretion of pro-inflammatory cytokines. CONCLUSION This is the first study to demonstrate that microglial activation state dictates EV uptake mechanisms and to establish a direct link between inflammation, cellular and membrane mechanics, and EV-mediated modulation. Our findings highlight RJEVs as promising candidates for regulating neuroinflammation by targeting microglial mechanobiology as well as opening new strategies for EV-based therapeutics