Browsing by Author "Tapia, AA"

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    Secondary metabolite content in Fabiana imbricata plants and in vitro cultures
    (2004) Schmeda-Hirschmann, G; Jordan, M; Gerth, A; Wilken, D; Hormazabal, E; Tapia, AA
    A rapid in vitro propagation system leading to the formation of shoots, calli, roots, cell suspensions and plantlets was developed for the Andean medicinal plant Fabiana imbricata (Solanaceae). Massive propagation of shoots and roots was achieved by the temporary immersion system (TIS), morphogenesis and maintenance of cell suspensions by standard in vitro culture techniques. Oleanolic acid (OA), rutin, chlorogenic acid (CA) and scopoletin content in aerial parts of wild growing Fabiana imbricata plants as well as in plantlets regenerated in vitro, callus cultures, cell suspensions and biomass, obtained by the TIS system was assessed by HPLC.