Browsing by Author "Torres, A."
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- ItemAlcohol consumption during adolescence: A link between mitochondrial damage and ethanol brain intoxication(2017) Tapia, C.; Mira, R.; Torres, A.; Jara, C.; Pérez, M.; Vergara, E.; Cerpa Nebott, Waldo Francisco; Quintanilla Gómez, Rodrigo Arthur
- ItemEquilibrative nucleoside transporter 1 expression is downregulated by hypoxia in human umbilical vein endothelium(2005) Casanello, P.; Torres, A.; Sanhueza, F.; González, M.; Farías, M.; Gallardo, V.; Pastor-Anglada, M.; San Martín, R.; Sobrevía Luarte, Luis AlbertoReduced oxygen level (hypoxia) induces endothelial dysfunction and release of the endogenous nucleosideadenosine. Human umbilical vein endothelium (HUVEC) function in an environment with 3% to 5% O2and exhibitefficient adenosine membrane transport via human equilibrative nucleoside transporters 1 (hENT1). We studied whetheradenosine transport and hENT1 expression are altered by hypoxia in HUVEC. Hypoxia (0 to 24 hours, 2% and 1% O2)reduced maximal hENT1-adenosine transport velocity (Vmax) and maximal nitrobenzylthionosine (NBMPR, a high-affinity hENT1 protein ligand) binding, but increased extracellular adenosine concentration. Hypoxia also reducedhENT1 protein and mRNA levels, effects unaltered byN_x0001_-nitro-L-arginine methyl ester (L-NAME, nitric oxide synthase[NOS] inhibitor) or PD-98059 (inhibitor of mitogen-activated protein kinase kinase 1 and 2 [MEK1/2]). Hypoxiareduced endothelial NOS (eNOS) activity and eNOS phosphorylation at Ser1177, but increased eNOS protein level.Hypoxia increased (1 to 3 hours), but reduced (24 hours) p42/44mapkphosphorylation. Thus, hypoxia-increasedextracellular adenosine may result from reduced hENT1-adenosine transport in HUVEC. Hypoxia effect seems not toinvolve NO, but p42/44mapkmay be required for the relatively rapid effect (1 to 3 hours) of hypoxia. These results couldbe important in diseases where the fetus is exposed to intrauterine environments poor in oxygen, such as intrauterinegrowth restriction, or where adenosine transport is altered, such as gestational diabetes.
- ItemEstructura factorial de la escala DREEM en estudiantes de medicina chilenos(2015) Ortega, B.; Perez, V.; Ortiz, M.; Fasce, H.; Mccoll, C.; Torres, A.; Wright Navarrete, Ana Cecilia; Marquez, U.; Parra, P.
- ItemInterleukin-8 secreted by glioblastoma cells induces microvascular hyperpermeability through NO signaling involving S-Nitrosylation of VE-Cadherin and p120 in endothelial cells(2019) Guequen, A.; Zamorano, P.; Cordova, F.; Koning, T.; Torres, A.; Ehrenfeld, P.; Boric P., Mauricio; Salazar Onfray, F.; Gavard, J.; Durran, W.N.
- ItemMultidrug resistance in glioblastoma stem-like cells: Role of the hypoxic microenvironment and adenosine signaling(2017) Uribe, D.; Torres, A.; Rocha, J.; Niechi, I.; Oyarzun, C.; Sobrevía Luarte, Luis Alberto; San Martin, R.; Quezada, C.
- ItemOsteoarthritis in Latin America study of demographic and clinical characteristics in 3040 patients(2015) Reginato, A.; Riera, H.; Vera, M.; Torres, A.; Espinosa, R.; Esquivel, J.; Felipe, O.; Blas, J.; Rillo, O.; Radrigán Araya, Francisco José Ricardo; Souto, R.; Rossi, C.; Molina, J.; Ballesteros, F.; Papasidero, S.; Guibert, M.
- ItemReactivity and selectivity of the reaction of O,O-diethyl 2,4-dinitrophenyl phosphate and thionophosphate with thiols of low molecular weight(2016) Santos, J. G.; Aliaga Miranda, Margarita Elly; Alarcón, K.; Torres, A.; Céspedes, D.; Pavez Guerrero, Paulina Isabel