Browsing by Author "Velasquez, Patricia"

Now showing 1 - 9 of 9
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    Antibacterial potential assessment of Nalca (Gunnera tinctoriaMol.) ethanolic extracts
    (2021) Velasquez, Patricia; Riquelme, Karla; Leyton, Felipe; Giordano, Ady; Gomez, Miguel; Montenegro, Gloria
    Nalca is an edible rhubarb-type plant from the southern of Chile; with studies focussing on petiole chemical and biological properties. This work evaluated for the first time the antibacterial capacities of three organs of nalca againstEscherichia coli,Staphylococcus aureusandPseudomonas aeruginosa. Petiole (P), root (R) and flower (F) ethanolic extracts at three different concentrations (25, 50, 100 mg/mL) were evaluated by means of agar diffusion assay and MIC. The result showed that antibacterial activities are bacteria and concentration dependent, with root having the highest antimicrobial activity of the organs plant studied. Compared to commercial antibiotics, nalca extracts show promising control overPs. aeruginosa. The main polyphenolics of the extracts were identified by UPLC-MS/MS, observing for the first time caffeic, coumaric and gallic acids presence in all samples, with quercetin and rutin determined in petiole and flower extracts only. Our results contribute to better understanding of nalca attributes as potential source of antibacterial compounds to be used in nutraceutical, cosmetic and food industry.
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    Bioactive polyphenols and antioxidant capacity of Azara petiolaris and Azara integrifolia Honeys
    (2018) Giordano Villatoro, Ady; Retamal, Mauricio; Leyton, Felipe; Martinez, Patricia; Bridi, Raquel; Velasquez, Patricia; Montenegro Rizzardini, Gloria
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    Biological activity of native Myrtaceae fruits from Chile as a potential functional food
    (2022) Velasquez, Patricia; Orellana, Jocelyn; Munoz-Carvajal, Eduardo; Faundez, Mario; Gomez, Miguel; Montenegro, Gloria; Giordano, Ady
    Myrtaceae fruits (Myrceugenia obtusa, Luma apiculata, and Luma chequen) were used as food and medicine by Chilean indigenous people. This study aimed to evaluate the bioactive properties of these berry-type fruits. The antioxidant capacity determined by the FRAP assay varied between 10.4 and 646.9 mmol Fe+2/g, while the antibacterial activity against Staphylococcus aureus and Salmonella typhi was 0 - 33 mm and 0 - 7.33 mm, respectively. All the extracts were rich in polyphenols and showed low cytotoxicity. Overall, M. obtusa presented dissimilar results compared to those of L. apiculata and L. chequen, encouraging the use of these native fruits as food, nutraceutical, or pharmacological ingredients.
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    Biological capacities of seven Chilean ferns
    (2024) Velasquez, Patricia; Trucco, Josefina; Munoz-Carvajal, Eduardo; Bustos, Daniela; Viteri, Rafael; Montenegro, Gloria; Giordano, Ady
    Sticherus squamulosus, Hymenophyllum dentatum, Blechnum microphyllum, Polypodium Feuillei, Blechnum chilense, Lophosoria quadripinnata, and Lissopimpla excelsa are native fern species found in southern Chile and are often used in traditional medicine. However, their bioactive properties have not been confirmed. In this study, ethanolic extracts of ferns exhibited significant antibacterial capacity against human pathogens. H. dentatum and S. squamulosus were selected for further analysis owing to their antimicrobial and antioxidant capacities. The entire H. dentatum extract exhibited fungistatic and fungicidal capacity on Botrytis cinerea and Penicillium digitatum, with minimum inhibitory concentration values of 1.56 mg/mL and 6.25 mg/mL and minimum fungicidal concentration values of 6.25 mg/mL and 12.5 mg/mL, respectively. The S. squamulosus extract presented the highest rutin content (222.5 mu g/g fern). In addition, the ethanolic extract of H. dentatum inhibited 91% of DPPH radicals. Therefore, both types of ferns can be used in the food industry.
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    Changes in the antibacterial capacity of Ulmo honey in relation to the contribution of Eucryphia cordifolia pollen
    (VUP FOOD RESEARCH INST, BRATISLAVA, 2021) Montenegro, Gloria; Velasquez, Patricia; Viteri, Rafael; Giordano, Ady
    Ulmo honey is known for its antibacterial characteristics. However, the importance of the content from Eucryphia cordifolia in the honey regarding its non-peroxidic antibacterial capacity is unknown. The present work evaluated the antibacterial activity of Ulmo honey with various percentages of pollen from E. cordifolia against various bacteria, determine the non-peroxidic capacity of Ulmo honey and, finally, compare that capacity with Manuka and Jarrah honeys. The antibacterial activity was evaluated by agar diffusion test and the non-peroxide capacity was evaluated by the reactivity with the catalase enzyme. The tests were carried out against Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes. The results showed a relationship between the percentage of floral pollen from E. cordifolia and the antibacterial activity of Ulmo honey. It was also observed than the antibacterial activity of Ulmo honey can have values similar to Manuka and Jarrah honeys.
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    Influence ofBrassica campestrisandGalega officinalison Antioxidant Activity of Bee Pollen
    (2020) Munoz, Eduardo; Velasquez, Patricia; Rodriguez, Karina; Montenegro, Gloria; Giordano, Ady
    The presence of polyphenol compounds in bee pollen differs with the floral species that comprise the pollen. These polyphenols are thus responsible for the free radical scavenging ability that the pollen exhibits. To correlate the botanical origin with the polyphenol content of Chilean bee pollen, samples were extracted in methanol using ultrasound-assisted extraction. Total polyphenol content, total flavonoid content, and antioxidant capacity were determined in all samples along with the polyphenol HPLC profile. According to the melissopalynological analysis, samples were either monofloral or multifloral bee pollen, with total polyphenol content between 7.7 and 22mg eq gallic acid/g of bee pollen and total flavonoid content between 76 and 296mg quercetin/kg pollen. All extracts showed an antioxidative activity for the ABTS radical of 23.9 to 43 mg eq trolox/g of bee pollen, and lower values for DPPH scavenging capacity (0.98 to 4.74 mg eq trolox/g of bee pollen). Coumaric acid and caffeic acid were present in all bee pollen, but different polyphenol profiles were observed among the samples. Results show a high correlation between the properties exhibited and the relative amount of pollen fromBrassica campestris and Galega officinalis in the sample, indicating a dependence of the bioactivity of bee pollen with the botanical origin and chemical composition.
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    Mayten Tree Seed Oil: Nutritional Value Evaluation According to Antioxidant Capacity and Bioactive Properties
    (2021) Ginocchio, Rosanna; Munoz-Carvajal, Eduardo; Velasquez, Patricia; Giordano, Ady; Montenegro, Gloria; Colque-Perez, German; Saez-Navarrete, Cesar
    The Mayten tree (Maytenus boaria Mol.), a native plant of Chile that grows under environmentally limiting conditions, was historically harvested to extract an edible oil, and may represent an opportunity to expand current vegetable oil production. Seeds were collected from Mayten trees in north-central Chile, and seed oil was extracted by solvent extraction. The seed oil showed a reddish coloration, with quality parameters similar to those of other vegetable oils. The fatty acid composition revealed high levels of monounsaturated and polyunsaturated fatty acids. Oleic and linoleic acids, which are relevant to the human diet, were well represented in the extracted Mayten tree seed oil. The oil displayed an antioxidant capacity due to the high contents of antioxidant compounds (polyphenols and carotenoids) and may have potential health benefits for diseases associated with oxidative stress.
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    Nutritional and antioxidant potential of Chilean native fruits: lleuque (Prumnopitys andina) and copihue (Lapageria rosea)
    (VUP FOOD RESEARCH INST, BRATISLAVA, 2021) Giovagnoli vicuna, Claudia; Velasquez, Patricia; Montenegro, Gloria; Espejo, Jaime; Gomez, Miguel; Cabrera barjas, Gustavo; Giordano, Ady
    Nutritional and antioxidant properties of pulp and seeds of lleuque (Prumnopitys andina) and copihue (Lapageria rosea) were investigated. Proximal analysis revealed that the pulps are rich in carbohydrates, whereas the seeds are a good source of fibre. Both pulps exhibited higher phenolics content (PC) and flavonoids content (FC), as well as antioxidant capacities, compared to seeds. The data showed that PC correlated with antioxidant capacity the strongest, followed by FC and anthocyanins content. It was observed that global antioxidant score and relative antioxidant capacity index represented the antioxidant capacity behaviour of the extracts. According to the polyphenol antioxidant coefficients calculation, PC was an important contributor to antioxidant capacity of all extracts. The results of this study confirmed that the native Chilean fruits lleuque and copihue are an excellent source of antioxidants.
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    Rapid Scanning of the Origin and Antioxidant Potential of Chilean Native Honey Through Infrared Spectroscopy and Chemometrics
    (2019) Giordano, Ady; Retamal, Mauricio; Fuentes, Edwar; Ascar, Loreto; Velasquez, Patricia; Rodriguez, Karina; Montenegro, Gloria
    Antioxidant compounds have the ability to trap free radicals; in honey, this capacity is related to the botanical origin of the sample, and therefore, there has been a growing interest in verifying the floral origin of beehive products and its relation with the polyphenolic compounds with potential antioxidant activity. A FTIR spectrum has been use to discriminate floral origin in Chilean monofloral samples and to predict their antioxidant capacity. Forty-nine honey samples from different geographical zones and botanical origin were classified according to melissopalynology analysis, and total phenolic and flavonoid contents were quantified by spectrophotometric methods. Discriminant analysis showed that Quillay (Quillaja saponaria), Corcolen (Azara petiolaris), and Tebo (Retanilla trinervia) honeys showed similarities related to their common geographical origin, while Ulmo (Eucryphia cordifolia) presents a differentiate behavior. The FTIR spectra were able to predict phenolic and flavonoid content, establishing the potential of spectroscopic tools for quality control in Chilean beehive industry.