Browsing by Author "Yuri, Jose A."

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    Antiplatelet, anticoagulant, and fibrinolytic activity in vitro of extracts from selected fruits and vegetables
    (LIPPINCOTT WILLIAMS & WILKINS, 2011) Torres Urrutia, Constanza; Guzman, Luis; Schmeda Hirschmann, Guillermo; Moore Carrasco, Rodrigo; Alarcon, Marcelo; Astudillo, Luis; Gutierrez, Margarita; Carrasco, Gilda; Yuri, Jose A.; Aranda, Eduardo; Palomo, Ivan
    A diet rich in fruits and vegetables is known to decrease the risk of cardiovascular disease. However, the information regarding the antithrombotic activity (antiplatelet, anticoagulant, and fibrinolytic) of fruits and vegetables is scarce. The aim of this study was to assess the antithrombotic activity of extracts from fruits and vegetables widely consumed in central Chile. The study included samples of 19 fruits and 26 vegetables, representative of the local diet. The extracts prepared from each sample included an aqueous (juice or pressed solubles) and/or methanol-soluble fraction. The extracts were evaluated for antiplatelet, anticoagulant, and fibrinolytic activity in vitro at a final concentration of 1 mg/ml. The antiplatelet activity was assessed by platelet aggregation inhibition; anticoagulant activity was measured by the prothrombin time (PT), diluted prothrombin time (dPT), activated partial thromboplastin time (APTT), kaolin clotting time (KCT), and thrombin time. The fibrinolytic effect was determined with the euglobin clot lysis time and fibrin plate methods. Extracts of green beans and tomatoes inhibited platelet aggregation induced by ADP and arachidonic acid, in a concentration-dependent manner. The methanolic extracts of grapes prolonged the PT and dPT. Finally, extracts of raspberry prolonged the APTT and also presented fibrinolytic activity. In conclusion, from a screening that included a variety of fruits and vegetables, we found antiplatelet activity in green beans and tomatoes, anticoagulant activities in grapes and raspberries, whereas fibrinolytic activity was observed only in raspberries. Further investigations are necessary to advance in knowledge of the active compounds of these fruits and vegetables and their mechanisms of action. Blood Coagul Fibrinolysis 22:197-205 (c) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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    Effect of cultivar, rootstock, and growing conditions on fruit maturity and postharvest quality as part of a six-year apple trial in Chile
    (2019) Yuri, Jose A.; Moggia, Claudia; Sepulveda, Alvaro; Poblete-Echeverria, Carlos; Valdes-Gomez, Hector; Torres, Carolina A.
    Fruit export is an important economic activity for Chile. Therefore, high productivity and quality fruit is a must in order to succeed in the business. A 6-year project was carried out to assess apple cultivars ('Galaxy', 'Brookfield (R) Gala', 'Super Chief, 'Fuji Raku Raku', 'Braebum', 'Granny Smith', and 'Cripps Pink') and rootstock's (M.M.106 and M.9 EMLA) performance across different Chilean apple-growing areas (Graneros, San Clemente, Chillan, Angol, and Temuco). Fruit maturity (firmness, soluble solids, starch degradation, internal ethylene concentration, and skin color) pre-and-postharvest was assessed weekly starting one-month prior harvest and monthly postharvest during years 4, 5, and 6 of the trial. Physiological disorders were also evaluated monthly for 6 months in RA (0-1oC, > 95% RH) storage. Based on bioclimatic variables, growing sites showed a clear distribution pattern on all principal component analyses, and it was more relevant than the growing season. In general, red skin color of most cultivars increased in sites towards the south of the country (cooler), regardless of the rootstock. Overall fruit firmness increased towards the southern sites in some cultivars, although not always statistically different. The highest softening rates pre-harvest were observed in 'Galaxy' and 'Brookfield (R) Gala'. In 'Galaxy', the lowest softening rate was observed in Temuco (coolest site). Brookfield (R) Gala' and 'Galaxy' apples grown in warmer sites (San Clemente and Angol) showed a sharper increase in internal ethylene concentration (IEC) than cooler sites (Temuco and Chillan) earlier in the season. Fruit quality and condition postharvest is also discussed.