Browsing by Author "Zanocco, Antonio L."

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    3-Hydroxykynurenine bound to eye lens proteins induces oxidative modifications in crystalline proteins through a type I photosensitizing mechanism
    (2019) Avila, Felipe; Ravello, Natalia; Zanocco, Antonio L.; Gamon, Luke F.; Davies, Michael J.; Silva, Eduardo
    Photosensitized reactions mediated by endogenous chromophores have been associated with the etiology of age-related cataract disease. Endogenous chromophores such as 3-hydroxykynurenine (3OHKN) can be found in both free form, and bound to crystallin proteins. However, their efficiency in generating photo-induced oxidative modifications on eye lens proteins is not completely understood. In this work, the efficiency and photodynamic activity of 3OHKN bound to both lysine (3OHKN-Lys) and bovine lens proteins (3OHKN-BLP) was assessed and compared with the photosensitizing activity of the major chromophore arising from glucose degradation (GDC). The photosensitizing activity of 3OHKN-Lys, 3OHKN-BLP and GDC was characterized by measurement of singlet oxygen quantum yields, O-2 consumption, SDS-PAGE and amino acid analysis of the photo-oxidized proteins.
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    Photophysics and photochemistry of naphthoxazinone derivatives
    (2008) Nonell, Santi; Ferreras, Lourdes R.; Cañete Molina, Álvaro; Lemp, Else; Günther, German; Pizarro, Nancy; Zanocco, Antonio L.