Characterization of the MYB60 Gene of Phaseolus vulgaris and its Role in Regulating Stomatal Aperture

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dc.catalogadorpva
dc.contributor.advisorArce Johnson, Jorge Patricio
dc.contributor.authorMartínez Barradas, Vera Isabel
dc.contributor.otherPontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas
dc.contributor.otherPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
dc.date.accessioned2024-04-08T19:53:45Z
dc.date.available2024-04-08T19:53:45Z
dc.date.issued2024
dc.date.updated2024-04-05T20:45:22Z
dc.descriptionTesis (Doctor en Biotecnología Vegetal)--Pontificia Universidad Católica de Chile, 2024
dc.descriptionTesis (Doctor en Biotecnología Vegetal)--Université de Bordeaux, 2024
dc.description.abstractIn this thesis the Phaseolus vulgaris MYB60 gene was characterized, and it is pointed as a promising candidate to improve its tolerance to drought stress. The specific objectives were: Characterize the ‘Zorzal’ cultivar physiological and yield response to drought stress in greenhouse. Identify, clone, and characterize the MYB60 gene of Phaseolus vulgaris orthologous to Arabidopsis thaliana including its coding region and promoter. Establish an in vitro regeneration protocol for the “Zorzal” common bean cultivar and design gRNAs that can be furtherly used for its edition. Zorzal presented physiological traits associated with drought tolerance. However, these traits are insufficient to maintain a stable yield under severe drought stress at the pre-flowering stage.Phaseolus vulgaris has a PvMYB60 ortholog gene to the MYB60 of Arabidopsis thaliana, with stomata-specific expression that is involved in promoting stomatal aperture.An in vitro regeneration method was developed that allows successful fertile plant regeneration of ‘Zorzal’ common bean by direct organogenesis from embryonic axis and embryonic apical meristem explants, with regeneration rates of 58.3 and 21.1% respectively, that together with the designed gRNAs provide a potential tool for genetic manipulation of Zorzal cultivar and common bean in general.
dc.description.funderANID
dc.fechaingreso.objetodigital2024-04-05
dc.format.extent136 páginas
dc.fuente.origenAutoarchivo
dc.identifier.doi10.7764/TesisUC/BIO/84936
dc.identifier.urihttps://doi.org/10.7764/TesisUC/BIO/84936
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/84987
dc.information.autorucFacultad de Ciencias Biológicas; Arce Johnson, Jorge Patricio; S/I; 54718
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Martínez Barradas, Vera Isabel; S/I; 1092177
dc.language.isoen
dc.nota.accesocontenido completo
dc.rightsacceso abierto
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.titleCharacterization of the MYB60 Gene of Phaseolus vulgaris and its Role in Regulating Stomatal Aperture
dc.typetesis doctoral
sipa.codpersvinculados54718
sipa.codpersvinculados1092177
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