Browsing by Author "Garcia-Rio, Luis"

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    A comparative study on the cucurbit[7]uril-based indicator displacement assay for methyl Viologen. A theoretical and experimental perspective
    Pavez Guerrero, Paulina; Fierro Huerta, Angelica Maria; Rojas Guerrero, Mabel Natalia; Garcia-Rio, Luis; Dinamarca-Villarroel, Luis; Fuentealba Patino, Denis Alberto; Droguett Muñoz, Kevin Arturo; Santos Blanco, José Guillermo; Aliaga Miranda, Margarita Elly
    One colorimetric and one fluorescent-indicator displacement assay (IDA) were studied to assess their interaction with methyl viologen (MV), an herbicide commercially known as paraquat. The host–guest complexes formed by cucurbit[7]uril (CB[7]) and the 4-nitroaniline (NA) or Berberine (Be) dyes function as colorimetric- and fluorescent-IDA for MV, respectively. The CB[7]@NA and CB[7]@Be complexes were characterized by UV–vis, fluorescence spectroscopy, and dynamic simulations. Isothermal titration calorimetry (ITC) experiments and dynamic simulations were employed to obtain thermodynamic data for the formation of the 1:1 inclusion complexes. Finally, NA is proposed as a reference colorimetric dye for determining CB[7]-binding affinities using competitive host–guest titrations, and the fluorescent-IDA as the best assay proposed, as it exhibits a high binding constant, a good detection limit of 2.7 × 10−6 mol L-1, good linearity in the range from 4 × 10−6 mol L-1 to 3.5 × 10−5 mol L-1, in aqueous solution.
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    Host–guest interaction of coumarin-derivative dyes and cucurbit[7]uril : leading to the formation of supramolecular ternary complexes with mercuric ions
    (2015) Aliaga Miranda, Margarita Elly; Garcia-Rio, Luis; Pessego, Marcia; Montecinos Escobar, Rodrigo Andrés; Fuentealba Patiño, Denis Alberto; Uribe, Ivan; Martin-Pastor, Manuel; Garcia-Beltran, Olimpo
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    Kinetic and mechanistic study of the reactions of aryl chloroformates with quinuclidines
    (2006) Castro R., Enrique; Aliaga Miranda, Margarita Elly; Campodónico Galdames, Paola Rossana; Leis, J. Ramon; Garcia-Rio, Luis; Santos Blanco, José Guillermo