Importance of different <i>tfd</i> genes for degradation of chloroaromatics by <i>Ralstonia eutropha</i> JMP134

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dc.contributor.authorPlumeier, I
dc.contributor.authorPérez-Pantoja, D
dc.contributor.authorHeim, S
dc.contributor.authorGonzález, B
dc.contributor.authorPieper, DH
dc.date.accessioned2025-01-21T01:30:22Z
dc.date.available2025-01-21T01:30:22Z
dc.date.issued2002
dc.description.abstractThe tfdC(I)D(I)E(I)F(I), and tfdD(II)C(II)E(II)F(II) gene modules of plasmid pJP4 of Ralstonia eutropha JMP134 encode complete sets of functional enzymes for the transformation of chlorocatechols into 3-oxoadipate, which are all expressed during growth on 2,4-dichlorophenoxyacetate (2,4-D). However, activity of tfd(1)-encoded enzymes was usually higher than that of tfd(II)-encoded enzymes, both in the wild-type strain grown on 2,4-D and in 3-chlorobenzoate-grown derivatives harboring only one tfd gene module. The tfdD(II)-encoded chloromuconate cycloisomerase exhibited special kinetic properties, with high activity against 3-chloromuconate and poor activity against 2-chloromuconate and unsubstituted muconate, thus explaining the different phenotypic behaviors of R. eutropha strains containing different tfd gene modules. The enzyme catalyzes the formation of an equilibrium between 2-chloromuconate and 5-chloro- and 2-chloromuconolactone and very inefficiently catalyzes dehalogenation to form trans-dienelactone as the major product, thus differing from all (chloro)muconate cycloisomerases described thus far.
dc.fuente.origenWOS
dc.identifier.doi10.1128/JB.184.15.4054-4064.2002
dc.identifier.eissn1098-5530
dc.identifier.issn0021-9193
dc.identifier.urihttps://doi.org/10.1128/JB.184.15.4054-4064.2002
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96720
dc.identifier.wosidWOS:000176853500002
dc.issue.numero15
dc.language.isoen
dc.pagina.final4064
dc.pagina.inicio4054
dc.revistaJournal of bacteriology
dc.rightsacceso restringido
dc.titleImportance of different <i>tfd</i> genes for degradation of chloroaromatics by <i>Ralstonia eutropha</i> JMP134
dc.typeartículo
dc.volumen184
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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