In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents
dc.contributor.author | Guzman, Leda | |
dc.contributor.author | Villalon, Katherine | |
dc.contributor.author | Jose Marchant, Maria | |
dc.contributor.author | Elena Tarnok, Maria | |
dc.contributor.author | Cardenas, Pilar | |
dc.contributor.author | Aquea, Gisela | |
dc.contributor.author | Acevedo, Waldo | |
dc.contributor.author | Padilla, Leandro | |
dc.contributor.author | Bernal, Giuliano | |
dc.contributor.author | Molinari, Aurora | |
dc.contributor.author | Corvalan, Alejandro | |
dc.date.accessioned | 2024-01-10T13:14:06Z | |
dc.date.available | 2024-01-10T13:14:06Z | |
dc.date.issued | 2020 | |
dc.description.abstract | The cytotoxic mechanism of the saponin QS-21 and its aglycone quillaic acid (QA) was studied on human gastric cancer cells (SNU1 and KATO III). Both compounds showed in vitro cytotoxic activity with IC50 values: 7.1 mu M (QS-21) and 13.6 mu M (QA) on SNU1 cells; 7.4 mu M (QS-21) and 67 mu M (QA) on KATO III cells. QS-21 and QA induce apoptosis on SNU1 and KATO III, as demonstrated by TUNEL, Annexin-V and Caspase Assays. Additionally, we performed in silico docking studies simulating the binding of both triterpenic compounds to key proteins involved in apoptotic pathways. The binding energies (G(bin)) thus calculated, suggest that the pro-apoptotic protein Bid might be a plausible target involved in the apoptotic effect of both triterpenic compounds. Although QA shows some antiproliferative effects on SNU1 cells cultured in vitro, our results suggest that QS-21 is a more powerful antitumor agent, which merits further investigation regarding their properties as potential therapeutic agents for gastric cancer. | |
dc.description.funder | Direccion de Investigacion de la Vicerrectoria de Investigacion y Estudios Avanzados | |
dc.description.funder | Pontificia Universidad Catolica de Valparaiso, Chile | |
dc.description.funder | CONICYT-FONDAP | |
dc.description.funder | CONICY | |
dc.description.funder | DESERT KING Chile | |
dc.fechaingreso.objetodigital | 2024-05-09 | |
dc.format.extent | 10 páginas | |
dc.fuente.origen | WOS | |
dc.identifier.doi | 10.1038/s41598-020-67442-3 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.pubmedid | MEDLINE:32601436 | |
dc.identifier.uri | https://doi.org/10.1038/s41598-020-67442-3 | |
dc.identifier.uri | https://repositorio.uc.cl/handle/11534/78373 | |
dc.identifier.wosid | WOS:000548359400007 | |
dc.information.autoruc | Arquitectura;Corvalan Rodriguez Alejandro;S/I;63885 | |
dc.information.autoruc | Ciencias Biológicas;Guzman Maluenda Leda;S/I;104061 | |
dc.information.autoruc | Ciencias Biológicas;Padilla Iglesias Leandro Mauri;S/I;93966 | |
dc.issue.numero | 1 | |
dc.language.iso | en | |
dc.nota.acceso | Sin adjunto | |
dc.publisher | NATURE RESEARCH | |
dc.revista | SCIENTIFIC REPORTS | |
dc.rights | registro bibliográfico | |
dc.subject | CELL-DEATH | |
dc.subject | APOPTOSIS | |
dc.subject | SAPONINS | |
dc.subject | GROWTH | |
dc.subject | FAS | |
dc.subject | STAUROSPORINE | |
dc.subject | INDUCTION | |
dc.subject | LIFE | |
dc.subject.ods | 03 Good Health and Well-being | |
dc.subject.odspa | 03 Salud y bienestar | |
dc.title | In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents | |
dc.type | artículo | |
dc.volumen | 10 | |
sipa.codpersvinculados | 63885 | |
sipa.codpersvinculados | 104061 | |
sipa.codpersvinculados | 93966 | |
sipa.index | WOS | |
sipa.index | Scopus | |
sipa.trazabilidad | Carga SIPA;09-01-2024 |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- In vitro evaluation and molecular.pdf
- Size:
- 3.31 MB
- Format:
- Adobe Portable Document Format
- Description: