<i>In vivo</i> expression of β-galactosidase by rat oviduct exposed to naked DNA or messenger RNA

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dc.contributor.authorRios, M
dc.contributor.authorVenegas, A
dc.contributor.authorCroxatto, HB
dc.date.accessioned2025-01-21T01:30:34Z
dc.date.available2025-01-21T01:30:34Z
dc.date.issued2002
dc.description.abstractIntra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (Rios et al., 1997). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 mug of pure beta-galactosidase (beta-gal) mRNA, 50 mug of pure DNA from the reporter gene beta-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the beta-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-beta-D-galactopyranoside as a substrate. The administration of beta-gal mRNA and pSVBgal plasmid increased beta-gal activity by 71% and 142%, respectively, over the control oviducts. These results indicate that naked DNA and mRNA coding for beta-gal can enter oviductal cells and be translated into an active enzyme. They are consistent with the interpretation that embryo transport acceleration caused by the injection of estradiol-induced RNA in the oviduct involves translation of the injected mRNA.
dc.fuente.origenWOS
dc.identifier.issn0716-9760
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96815
dc.identifier.wosidWOS:000179238700002
dc.issue.numero3-4
dc.language.isoen
dc.pagina.final338
dc.pagina.inicio333
dc.revistaBiological research
dc.rightsacceso restringido
dc.subjectbeta-galactosidase
dc.subjectgene transfer
dc.subjectoviduct
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.title<i>In vivo</i> expression of β-galactosidase by rat oviduct exposed to naked DNA or messenger RNA
dc.typeartículo
dc.volumen35
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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