Transfer of the mitochondrial <i>rps10</i> gene to the nucleus in rice

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dc.contributor.authorKubo, N
dc.contributor.authorJordana, X
dc.contributor.authorOzawa, K
dc.contributor.authorZanlungo, S
dc.contributor.authorHarada, K
dc.contributor.authorSasaki, T
dc.contributor.authorKadowaki, K
dc.date.accessioned2025-01-21T01:31:24Z
dc.date.available2025-01-21T01:31:24Z
dc.date.issued2000
dc.description.abstractMitochondrial ribosomal protein S10 (rps10) is encoded by the mitochondrial genome in potato and pea. Here we show that the rps10 gene is absent from the mitochondrial genome of rice and has been transferred to the nucleus. Cloning and transcriptional analysis show that there are two rps10 genes in the rice nuclear genome and that their transcripts differ in abundance. Western analysis detected the RPS10 protein in the soluble fraction of rice mitochondrial although neither RPS10 has any obvious N-terminal presequence for targeting to mitochondria. This result suggests that targeting information is present in the internal region of rice RPS10. Genomic sequence analysis indicated that each rps10 gene has an intron in the 5' untranslated region (5' UTR) and that these intron sequences are homologous to each other. This result strongly suggests that a duplication event occurred after transfer of the rps10 gene to the nucleus. The duplicated rps10 genes have since been translocated to different chromosomes, because the two rps10 genes were mapped on chromosomes 6 and 12 by RFLP analysis. Interestingly, the 5' UTR and the intron of the rice rps10 genes are homologous to sequences found in several rice genes with various functions, such as osk4, EF-1 beta 2 and RAG1, suggesting a common origin and a functional role for the 5' UTR. Acquisition of the 5' flanking region-might have accelerated the activation of the mitochondrial rps10 gene which was transferred to the nuclear genome.
dc.fuente.origenWOS
dc.identifier.issn0026-8925
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97055
dc.identifier.wosidWOS:000087452100021
dc.issue.numero4
dc.language.isoen
dc.pagina.final739
dc.pagina.inicio733
dc.revistaMolecular and general genetics
dc.rightsacceso restringido
dc.subjectmitochondria
dc.subjectgene transfer
dc.subjectribosomal protein S10
dc.subjectrice
dc.subject5 ' untranslated region (UTR)
dc.subject.ods13 Climate Action
dc.subject.odspa13 Acción por el clima
dc.titleTransfer of the mitochondrial <i>rps10</i> gene to the nucleus in rice
dc.typeartículo
dc.volumen263
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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