Assessing the Effect of Deep Eutectic Solvents on α-Chymotrypsin Thermal Stability and Activity

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dc.article.numbere202401414
dc.catalogadorgjm
dc.contributor.authorGajardo-Parra, Nicolás F.
dc.contributor.authorCea-Klapp, Esteban
dc.contributor.authorChandra, Anshu
dc.contributor.authorCanales Muñoz, Roberto
dc.contributor.authorGarrido, José Matías
dc.contributor.authorHeld, Christoph
dc.contributor.authorGuajardo Ramírez, Nadia Verónica
dc.date.accessioned2025-03-20T13:04:05Z
dc.date.available2025-03-20T13:04:05Z
dc.date.issued2024
dc.description.abstractOptimizing the liquid reaction phase holds significant potential for enhancing the efficiency of biocatalytic processes since it determines reaction equilibrium and kinetics. This study investigates the influence of the addition of deep eutectic solvents on the stability and activity of α-chymotrypsin, a proteolytic enzyme with industrial relevance. Deep eutectic solvents, composed of choline chloride or betaine mixed with glycerol or sorbitol, were added in the reaction phase at various concentrations. Experimental techniques, including kinetic and fluorometry, were employed to assess the α-chymotrypsin activity, thermal stability, and unfolding reversibility. Atomistic molecular dynamics simulations were also conducted to assess the interactions and provide molecular-level insights between α-chymotrypsin and the solvent. The results showed that among all studied mixtures, adding choline chloride + sorbitol improved thermal stability up to 18 °C and reaction kinetic efficiency up to two-fold upon adding choline chloride + glycerol. Notably, the choline chloride + sorbitol system exhibited the most substantial stabilization effect, attributed to the surface preferential accumulation of sorbitol, as corroborated by the computational analyses. This work highlights the potential of tailoring liquid reaction phase of α-chymotrypsin catalyzed reaction using neoteric solvents such as deep eutectic solvents to enhance α-chymotrypsin performance and stability in industrial applications.
dc.description.funderNational Agency for Research and Development
dc.description.funderFONDECYT
dc.description.funderANID
dc.format.extent9 páginas
dc.fuente.origenSCOPUS
dc.identifier.doi10.1002/cssc.202401414
dc.identifier.issn1864-564X
dc.identifier.scopusidSCOPUS_ID:85210175112
dc.identifier.urihttps://doi.org/10.1002/cssc.202401414
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102855
dc.information.autorucEscuela de Ingeniería; Canales Muñoz, Roberto; 0000-0002-2535-6527; 1039369
dc.information.autorucEscuela de Ingeniería; Guajardo Ramírez, Nadia Verónica; S/I; 1334439
dc.issue.numero5
dc.language.isoen
dc.nota.accesocontenido parcial
dc.revistaChemSusChem
dc.rightsacceso restringido
dc.subjectDeep Eutectic Solvents
dc.subjectPreferential hydration
dc.subjectProtein unfolding
dc.subjectSolvation
dc.subjectThermal stability
dc.subject.ddc600
dc.subject.deweyTecnologíaes_ES
dc.titleAssessing the Effect of Deep Eutectic Solvents on α-Chymotrypsin Thermal Stability and Activity
dc.typeartículo
dc.volumen18
sipa.codpersvinculados1039369
sipa.codpersvinculados1334439
sipa.trazabilidadSCOPUS;2024-12-08
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