Transcriptomic profiles reveal differences in zinc metabolism, inflammation, and tight junction proteins in duodenum from cholesterol gallstone subjects

Simple item page
dc.catalogadordfo
dc.contributor.authorRiveras Hernández, Eleodoro Javier
dc.contributor.authorAzócar, Lorena
dc.contributor.authorMoyano, Tomás C.
dc.contributor.authorOcares, Marcia
dc.contributor.authorMolina, Héctor
dc.contributor.authorRomero, Diego
dc.contributor.authorRoa Strauch, Juan Carlos Enrique
dc.contributor.authorValbuena Mora, José Rafael
dc.contributor.authorGutiérrez, Rodrigo A.
dc.contributor.authorMiquel P., Juan Francisco
dc.date.accessioned2024-01-29T19:28:32Z
dc.date.available2024-01-29T19:28:32Z
dc.date.issued2020
dc.description.abstractCholesterol Gallstone Disease (GSD) is a common multifactorial disorder characterized by crystallization and aggregation of biliary cholesterol in the gallbladder. The global prevalence of GSD is similar to 10-20% in the adult population but rises to 28% in Chile (17% among men and 30% among women). The small intestine may play a role in GSD pathogenesis, but the molecular mechanisms have not been clarified. Our aim was to identify the role of the small intestine in GSD pathogenesis. Duodenal biopsy samples were obtained from patients with GSD and healthy volunteers. GSD status was defined by abdominal ultrasonography. We performed a transcriptome study in a discovery cohort using Illumina HiSeq. 2500, and qPCR, immunohistochemistry and immunofluorescence were used to validate differentially expressed genes among additional case-control cohorts. 548 differentially expressed genes between GSD and control subjects were identified. Enriched biological processes related to cellular response to zinc, and immune and antimicrobial responses were observed in GSD patients. We validated lower transcript levels of metallothionein, NPC1L1 and tight junction genes and higher transcript levels of genes involved in immune and antimicrobial pathways in GSD patients. Interestingly, serum zinc and phytosterol to cholesterol precursor ratios were lower in GSD patients. A significant association was observed between serum zinc and phytosterol levels. Our results support a model where proximal small intestine plays a key role in GSD pathogenesis. Zinc supplementation, modulation of proximal microbiota and/or intestinal barrier may be novel targets for strategies to prevent GSD.
dc.fuente.origenConveris
dc.identifier.doi10.1038/s41598-020-64137-7
dc.identifier.issn2045-2322
dc.identifier.pubmedidMEDLINE:32366946
dc.identifier.scopusid2-s2.0-85084238621
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/81043
dc.identifier.wosidWOS:000560030200017
dc.information.autorucFacultad de Ciencias Biológicas; Riveras Hernandez Eleodoro Javier; S/I; 197816
dc.information.autorucFacultad de Ciencias Biológicas; Moyano Yugovic Tomas Custodio; S/I; 149778
dc.information.autorucEscuela de Medicina; Roa Strauch Juan Carlos Enrique; 0000-0001-8313-8774; 84743
dc.information.autorucEscuela de Medicina; Valbuena Mora Jose Rafael; S/I; 1004589
dc.information.autorucEscuela de Medicina; Miquel Poblete Juan Francisco; 0000-0002-0526-4377; 72216
dc.issue.numero1
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final10
dc.pagina.inicio1
dc.revistaScientific Reports
dc.rightsacceso restringido
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTranscriptomic profiles reveal differences in zinc metabolism, inflammation, and tight junction proteins in duodenum from cholesterol gallstone subjects
dc.typeartículo
dc.volumen10
sipa.codpersvinculados197816
sipa.codpersvinculados149778
sipa.codpersvinculados84743
sipa.codpersvinculados1004589
sipa.codpersvinculados72216
sipa.trazabilidadConveris;20-07-2021
Files
Collections
Artículos de revistas