Small extracellular vesicles from pregnant women with maternal supraphysiological hypercholesterolemia impair endothelial cell function in vitro

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dc.contributor.authorContreras-Duarte, Susana
dc.contributor.authorEscalona-Rivano, Rodrigo
dc.contributor.authorCantin, Claudette
dc.contributor.authorValdivia, Pascuala
dc.contributor.authorZapata, David
dc.contributor.authorCarvajal, Lorena
dc.contributor.authorBrito, Roberto
dc.contributor.authorCerda, Alvaro
dc.contributor.authorIllanes, Sebastian
dc.contributor.authorGutierrez, Jaime
dc.contributor.authorLeiva, Andrea
dc.date.accessioned2025-01-20T20:10:41Z
dc.date.available2025-01-20T20:10:41Z
dc.date.issued2023
dc.description.abstractMaternal physiological hypercholesterolemia MPH, maternal total cholesterol (TC) levels at term of pregnancy <280 mg/dL) occurs to assure fetal development. Maternal supraphysiological hypercholesterolemia (MSPH, TC levels >280 mg/dL) is a pathological condition associated with maternal, placental, and fetal endothelial dysfunction and early neonatal atherosclerosis development. Small extracellular vesicles (sEVs) are delivered to the extracellular space by different cells, where they modulate cell functions by transporting active signaling molecules, including proteins and miRNA.Aim: To determine whether sEVs from MSPH women could alter the function of endothelial cells (angiogenesis, endothelial activation and nitric oxide synthesis capacity).Methods: This study included 24 Chilean women (12 MPH and 12 MSPH). sEVs were isolated from maternal plasma and characterized by sEV markers (CD9, Alix and HSP70), nanoparticle tracking analysis, transmission electron microscopy, and protein and cholesterol content. The endothelial cell line HMEC-1 was used to determine the uptake of labeled sEVs and the effects of sEVs on cell viability, endothelial tube formation, endothelial cell activation, and endothelial nitric oxide expression and function.Results: In MSPH women, the plasma concentration of sEVs was increased compared to that in MPH women. MSPH-sEVs were highly taken up by HMEC-1 cells and reduced angiogenic capacity and the expression and activity of eNOS without changing cell viability or endothelial activation compared to MPH-sEVs. Conclusion: sEVs from MSPH women impair angiogenesis and nitric oxide synthesis in endothelial cells, which could contribute to MSPH-associated endothelial dysfunction.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.vph.2023.107174
dc.identifier.eissn1879-3649
dc.identifier.issn1537-1891
dc.identifier.urihttps://doi.org/10.1016/j.vph.2023.107174
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92089
dc.identifier.wosidWOS:001001231600001
dc.language.isoen
dc.revistaVascular pharmacology
dc.rightsacceso restringido
dc.subjectCholesterol
dc.subjectPregnancy
dc.subjectSmall extracellular vesicles
dc.subjectEndothelial function
dc.subjectAngiogenesis
dc.subjectNitric oxide
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSmall extracellular vesicles from pregnant women with maternal supraphysiological hypercholesterolemia impair endothelial cell function in vitro
dc.typeartículo
dc.volumen150
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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