Triclosan inhibits tumor necrosis factor-alpha-stimulated urokinase production in human gingival fibroblasts

Simple item page
dc.contributor.authorArancibia, R.
dc.contributor.authorCaceres, M.
dc.contributor.authorMartinez, J.
dc.contributor.authorSmith, P. C.
dc.date.accessioned2024-01-10T14:22:18Z
dc.date.available2024-01-10T14:22:18Z
dc.date.issued2009
dc.description.abstractBackground and Objectives: Destruction of the supporting periodontal tissues is mediated by the action of several proteolytic enzymes. Urokinase is a serine protease that plays a key role in connective tissue destruction through conversion of plasminogen into plasmin. The present study was conducted to evaluate the effect of triclosan on the production and activity of urokinase in cultured gingival fibroblasts.
dc.description.abstractMaterial and Methods: Urokinase production was studied in primary cultures of human gingival fibroblasts stimulated with tumor necrosis factor-alfa. Urokinase activity and production were evaluated using casein zymography and western blotting, respecively. Urokinase mRNA expression was evaluated using the reverse transcription-polymerase chain reaction. Triclosan was used to interfere with this stimulatory effect. The roles of different cell-signaling cascades involved in urokinase production were assessed through western blotting and immunofluorescence using several cell-signaling inhibitors.
dc.description.abstractResults: Tumor necrosis factor-alfa was found to be a strong stimulus for urokinase production and triclosan was able to inhibit this response at the protein and mRNA levels. Triclosan was also able to inhibit conversion of plasminogen into plasmin. Tumor necrosis factor-alfa-stimulated urokinase production was shown to be dependent on the nuclear factor-kappa B and c-Jun N-terminal kinase signaling pathways. Triclosan inhibited c-Jun N-terminal kinase phosphorylation and c-Jun production.
dc.description.abstractConclusions: Within the limits of this study, these results show that triclosan may inhibit urokinase production and plasminogen activation in gingival fibroblasts through modulation of the c-Jun N-terminal kinase signaling pathway.
dc.description.funderFONDECYT (National Fund for Science and Technology, Chile)
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1111/j.1600-0765.2008.01184.x
dc.identifier.eissn1600-0765
dc.identifier.issn0022-3484
dc.identifier.pubmedidMEDLINE:19874453
dc.identifier.urihttps://doi.org/10.1111/j.1600-0765.2008.01184.x
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79907
dc.identifier.wosidWOS:000271062400006
dc.information.autorucMedicina;Smith PC;S/I;1006488
dc.issue.numero6
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final735
dc.pagina.inicio726
dc.publisherWILEY
dc.revistaJOURNAL OF PERIODONTAL RESEARCH
dc.rightsregistro bibliográfico
dc.subjectTriclosan
dc.subjectfibroblast
dc.subjecturokinase
dc.subjectgingival
dc.subjectNF-KAPPA-B
dc.subjectTRICLOSAN/COPOLYMER DENTIFRICE
dc.subjectPLASMINOGEN-ACTIVATOR
dc.subjectPERIODONTAL-DISEASE
dc.subjectCREVICULAR FLUID
dc.subjectEXPRESSION
dc.subjectPROGRESSION
dc.subjectPLAQUE
dc.subjectSECRETION
dc.subjectMEDIATORS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTriclosan inhibits tumor necrosis factor-alpha-stimulated urokinase production in human gingival fibroblasts
dc.typeartículo
dc.volumen44
sipa.codpersvinculados1006488
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Collections
Artículos de revistas