Mutations in <i>trpγ</i>, the homologue of <i>TRPC6</i> autism candidate gene, causes autism-like behavioral deficits in <i>Drosophila</i>

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dc.contributor.authorPalacios-Munoz, Angelina
dc.contributor.authorde Paula Moreira, Danielle
dc.contributor.authorSilva, Valeria
dc.contributor.authorGarcia, Isaac E.
dc.contributor.authorAboitiz, Francisco
dc.contributor.authorZarrei, Mehdi
dc.contributor.authorCampos, Gabriele
dc.contributor.authorRennie, Olivia
dc.contributor.authorHowe, Jennifer L.
dc.contributor.authorAnagnostou, Evdokia
dc.contributor.authorAmbrozewic, Patricia
dc.contributor.authorScherer, Stephen W.
dc.contributor.authorRita Passos-Bueno, Maria
dc.contributor.authorEwer, John
dc.date.accessioned2025-01-20T21:08:31Z
dc.date.available2025-01-20T21:08:31Z
dc.date.issued2022
dc.description.abstractAutism Spectrum Disorder (ASD) is characterized by impaired social communication, restricted interests, and repetitive and stereotyped behaviors. The TRPC6 (transient receptor potential channel 6) represents an ASD candidate gene under an oligogenic/multifactorial model based on the initial description and cellular characterization of an individual with ASD bearing a de novo heterozygous mutation disrupting TRPC6, together with the enrichment of disruptive TRPC6 variants in ASD cases as compared to controls. Here, we perform a clinical re-evaluation of the initial non-verbal patient, and also present eight newly reported individuals ascertained for ASD and bearing predicted loss-of-function mutations in TRPC6. In order to understand the consequences of mutations in TRPC6 on nervous system function, we used the fruit fly, Drosophila melanogaster, to show that null mutations in transient receptor gamma (trp gamma; the fly gene most similar to TRPC6), cause a number of behavioral defects that mirror features seen in ASD patients, including deficits in social interactions (based on courtship behavior), impaired sleep homeostasis (without affecting the circadian control of sleep), hyperactivity in both young and old flies, and defects in learning and memory. Some defects, most notably in sleep, differed in severity between males and females and became normal with age. Interestingly, hyperforin, a TRPC6 agonist and the primary active component of the St. John's wort antidepressant, attenuated many of the deficits expressed by trp gamma mutant flies. In summary, our results provide further evidence that the TRPC6 gene is a risk factor for ASD. In addition, they show that the behavioral defects caused by mutations in TRPC6 can be modeled in Drosophila, thereby establishing a paradigm to examine the impact of mutations in other candidate genes.
dc.fuente.origenWOS
dc.identifier.doi10.1038/s41380-022-01555-1
dc.identifier.eissn1476-5578
dc.identifier.issn1359-4184
dc.identifier.urihttps://doi.org/10.1038/s41380-022-01555-1
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93477
dc.identifier.wosidWOS:000789735500001
dc.issue.numero8
dc.language.isoen
dc.pagina.final3342
dc.pagina.inicio3328
dc.revistaMolecular psychiatry
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMutations in <i>trpγ</i>, the homologue of <i>TRPC6</i> autism candidate gene, causes autism-like behavioral deficits in <i>Drosophila</i>
dc.typeartículo
dc.volumen27
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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