The transcriptional repression of equilibrative nucleoside transporter 1 by D-glucose rfsponses to transcription factor Sp1 and ZBP-89 in human foetal endothelium

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dc.catalogadorpau
dc.contributor.authorPuebla Aracena, Carlos Alberto
dc.contributor.authorFarías Jofré, Marcelo Enrique
dc.contributor.authorVega Pizarro, José Luis Eduardo
dc.contributor.authorPastor-Anglada, M.
dc.contributor.authorCasanello Toledo, Paola Cecilia
dc.contributor.authorSobrevía Luarte, Luis Alberto
dc.date.accessioned2023-07-21T17:46:16Z
dc.date.available2023-07-21T17:46:16Z
dc.date.issued2008
dc.description.abstractObjectives: Adenosine is a vasodilator in most vascular beds, an effect depending on its extracellular concentration. Uptake of this nucleoside in human umbilical vein endothelial cells (HUVEC) is mainly mediated by human equilibrative nucleoside transporters 1 (hENT1). hENT1 expression and transport activity are reduced in HUVEC exposed to high D-glucose. Since specific mechanisms for these effects of D-glucose are unknown, we examined the role of Sp1 and BP-89 transcription factors on SLC29A1 (for hENT1) promoter activity in response to D-glucose. Methods: HUVEC from normal pregnancies were isolated and exposed (24 h) to 5 mM (normal) or 25 mM (high) D-glucose. Sp1 protein levels were evaluated by western blot in nuclear fractions. Reporter activity of plasmid constructs containing a wild type promoter region of SLC29A1 (-1114 bp to ATG, pGL3-hENT1-1114), or mutations (by PCR) for Sp1 (-815/-801 bp, pGL3-hENT1-1114mutSp1) or ZBP-89 (-992/-969 bp, pGL3-hENT1-1114mutZBP) or both (-1114 bp, pGL3-hENT1-1114mutSp1/ZBP) binding sites were assayed in cells over-expressing Sp1 (using pCGN-Sp1 vector). Results: Nuclear Sp1 abundance was increased, but pGL3-hENT1-1114 transcriptional activity was reduced by high D-glucose. Sp1 over-expression reduced pGL3-hENT1-1114 transcriptional activity in normal or high D-glucose. The effect of high D-glucose or Sp1 over-expression was absent in pGL3-hENT1-1114mutSp1, pGL3-hENT1-1114mutZBP and pGL3-hENT1-1114mutSp1/ZBP cells. Conclusion: A repression of the SLC29A1 promoter activity by Sp1 and ZBP-89 could explain the reduced hENT1 expression and activity exhibited by HUVEC in high extracellular D-glucose. FONDECYT 1070865/1080534/7070249 (Chile), AECI A/5484/06 (Spain). C Puebla andJL Vega hold CONICYT fellowships. M Farías holds CONICYT and PUC-School of Medicine PhD fellowships.
dc.format.extent1 página
dc.fuente.origenORCID
dc.identifier.doi10.1016/j.placenta.2008.06.002
dc.identifier.issn0143-4004
dc.identifier.urihttps://doi.org/10.1016/j.placenta.2008.06.002
dc.identifier.urihttps://publons.com/wos-op/publon/18766737/
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/74281
dc.information.autorucFacultad de Ciencias Biológicas ; Puebla Aracena, Carlos Alberto ; 0000-0001-6648-2135 ; 124965
dc.information.autorucEscuela de Medicina ; Farías Jofré, Marcelo Enrique ; 0000-0003-0473-2295 ; 12286
dc.information.autorucFacultad de Ciencias Biológicas ; Vega Pizarro, José Luis Eduardo ; 0000-0001-9333-8729 ; 165832
dc.information.autorucEscuela de Medicina ; Casanello Toledo, Paola Cecilia ; 0000-0002-2355-1476 ; 146772
dc.information.autorucEscuela de Medicina ; Sobrevía Luarte, Luis Alberto ; 0000-0001-5802-2243 ; 1002656
dc.issue.numero8
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.finalA18
dc.pagina.inicioA18
dc.relation.ispartofMeeting of the International-Federation-of-Placental-Associations (14° ; 2008 ; Seggau castle, Austria)
dc.revistaPlacentaes_ES
dc.rightsacceso restringido
dc.titleThe transcriptional repression of equilibrative nucleoside transporter 1 by D-glucose rfsponses to transcription factor Sp1 and ZBP-89 in human foetal endotheliumes_ES
dc.typecomunicación de congreso
dc.volumen29
sipa.codpersvinculados124965
sipa.codpersvinculados12286
sipa.codpersvinculados165832
sipa.codpersvinculados146772
sipa.codpersvinculados1002656
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