Chromosome-Mediated Colistin Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli: Mutation Analysis in the Light of Genetic Background

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dc.catalogadorjwg
dc.contributor.authorRiquelme, María Paz
dc.contributor.authorMartínez, Rodrigo W.
dc.contributor.authorBrito, Bárbara
dc.contributor.authorGarcía, Patricia
dc.contributor.authorWozniak Banchero, Aniela
dc.contributor.authorLegarraga, Paulette
dc.date.accessioned2024-01-26T12:48:30Z
dc.date.available2024-01-26T12:48:30Z
dc.date.issued2023
dc.description.abstract© 2023 Riquelme et al.Purpose: Colistin resistance mechanisms involving mutations in chromosomal genes associated with LPS modification are not completely understood. Mutations in genes coding for the MgrB regulator frequently account for colistin resistance in Klebsiella pneumoniae, whereas mutations in genes coding for PhoPQ and PmrAB are frequent in E. coli. Our aim was to perform a genetic analysis of chromosomal mutations in colistin-resistant (MIC ≥4 µg/mL) clinical isolates of K. pneumoniae (n = 8) and E. coli (n = 7) of different STs. Methods: Isolates were obtained in a 3-year period in a university hospital in Santiago, Chile. Susceptibility to colistin, aminoglyco-sides, cephalosporins, carbapenems and ciprofloxacin was determined through broth microdilution. Whole genome sequencing was performed for all isolates and chromosomal gene sequences were compared with sequences of colistin-susceptible isolates of the same sequence types. Results: None of the isolates carried mcr genes. Most of the isolates were susceptible to all the antibiotics analyzed. E. coli isolates were ST69, ST127, ST59, ST131 and ST14, and K. pneumoniae isolates were ST454, ST45, ST6293, ST380 and ST25. All the isolates had mutations in chromosomal genes analyzed. K. pneumoniae had mutations mainly in mgrB gene, whereas E. coli had mutations in pmrA, pmrB and pmrE genes. Most of the amino acid changes in LPS-modifying enzymes of colistin-resistant isolates were found in colistin-susceptible isolates of the same and/or different ST. Eleven of them were found only in colistin-resistant isolates. Conclusion: Colistin resistance mechanisms depend on genetic background, and are due to chromosomal mutations, which implies a lower risk of transmission than plasmid-mediated genes. Colistin resistance is not associated with multidrug-resistance, nor to high-risk sequence types.
dc.description.funderDepartment of Clinical Laboratories
dc.description.funderRed de Salud UC-Christus
dc.description.funderSENTRY
dc.description.funderPontificia Universidad Católica de Chile
dc.fechaingreso.objetodigital2024-06-06
dc.fuente.origenScopus
dc.identifier.doi10.2147/IDR.S427398
dc.identifier.issn11786973
dc.identifier.scopusidSCOPUS_ID:85173000994
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/80965
dc.information.autorucEscuela de Medicina; Wozniak Banchero Aniela; 0000-0001-9559-7660; 1008612
dc.language.isoen
dc.nota.accesoContenido completo
dc.pagina.final6462
dc.pagina.inicio6451
dc.publisherDove Medical Press Ltd
dc.revistaInfection and Drug Resistance
dc.rightsacceso abierto
dc.subjectSequence type
dc.subjectLPS-modifying enzymes
dc.subjectPolymorphism vs potential mutation
dc.subjectMgrB regulator
dc.subjectPmrA-PmrB and PhoP-PhoQ three-component systems
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleChromosome-Mediated Colistin Resistance in Clinical Isolates of Klebsiella pneumoniae and Escherichia coli: Mutation Analysis in the Light of Genetic Background
dc.typeartículo
dc.volumen16
sipa.codpersvinculados1008612
sipa.trazabilidadORCID;2024-01-15
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