Lymphoid B cells induce NF-kappa B activation in high endothelial cells from human tonsils

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dc.contributor.authorNaves, R
dc.contributor.authorReyes, LI
dc.contributor.authorRosemblatt, M
dc.contributor.authorJacobelli, S
dc.contributor.authorGonzalez, A
dc.contributor.authorBono, MR
dc.date.accessioned2024-01-10T13:50:10Z
dc.date.available2024-01-10T13:50:10Z
dc.date.issued2006
dc.description.abstractImmune surveillance depends on still poorly understood lymphocyte-endothelium interactions required for lymphocyte transendothelial migration into secondary lymphoid organs. The nuclear factor kappa B (NF-kappa B) regulatory system and its inhibitory I kappa B proteins control the inducible expression of adhesion molecules, cytokines and chemokines involved in endothelial activation and lymphocyte transmigration. Here we present results showing the activation of this system in response to the interaction of high endothelial cells from human tonsils (HUTEC) with human B and T lymphoid cell lines and primary tonsillar lymphocytes. Western blot and electrophoretic mobility shift assays show that adhesion of different lymphoid cells induce varying levels of NF-kappa B activation in HUTEC, with Daudi cells, tonsil-derived B cell line 10 (TBCL-10) and primary tonsillar B lymphocytes causing the strongest activation. The main NF-kappa B protein complexes translocated to the nucleus were p65/p50 and p50/p50. Results from reverse transcription-PCR and flow cytometry analysis of HUTEC indicate that the interaction with Daudi cells induce an increased expression of IL-6 and IL-8 mRNA and cell-surface expression of intercellular adhesion molecule-1, all of which were prevented by sodium salicylate, an inhibitor of NF-kappa B activation. Transwell experiments show that NF-kappa B activation and the response of HUTEC to the interaction of Daudi cells does not depend on direct cell-cell contact but rather on the production of soluble factors that require the presence of both cell types. These results suggest that lymphocytes and high endothelium establish a cross talk leading to NF-kappa B-mediated expression of cytokines and adhesion molecules, inducing endothelial cell activation.
dc.fechaingreso.objetodigital2024-05-10
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1093/intimm/dxh365
dc.identifier.eissn1460-2377
dc.identifier.issn0953-8178
dc.identifier.pubmedidMEDLINE:16373365
dc.identifier.urihttps://doi.org/10.1093/intimm/dxh365
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79509
dc.identifier.wosidWOS:000234967000005
dc.information.autorucMedicina;Jacobelli S;S/I;98345
dc.issue.numero2
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final267
dc.pagina.inicio259
dc.publisherOXFORD UNIV PRESS
dc.revistaINTERNATIONAL IMMUNOLOGY
dc.rightsregistro bibliográfico
dc.subjectcytokines
dc.subjectendothelial activation
dc.subjecthigh endothelium
dc.subjectNF-kappa B
dc.subjectADHESION MOLECULE EXPRESSION
dc.subjectLEUKOCYTE ADHESION
dc.subjectTRANSCRIPTIONAL REGULATION
dc.subjectTYROSINE PHOSPHORYLATION
dc.subjectTRAFFIC SIGNALS
dc.subjectIL-6 PRODUCTION
dc.subjectC-REL
dc.subjectINHIBITION
dc.subjectCONTACT
dc.subjectGENE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleLymphoid B cells induce NF-kappa B activation in high endothelial cells from human tonsils
dc.typeartículo
dc.volumen18
sipa.codpersvinculados98345
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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