Low Expression of Vitamin D Receptor in Patients With Dry Eye Disease

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dc.contributor.authorVieira, Antonia
dc.contributor.authorMeza, Javiera
dc.contributor.authorGarreton, Rodolfo
dc.contributor.authorGrau, Arturo E.
dc.contributor.authorZoroquiain, Pablo
dc.date.accessioned2025-01-20T16:04:28Z
dc.date.available2025-01-20T16:04:28Z
dc.date.issued2024
dc.description.abstractPurpose:This study aimed to compare the expression of vitamin D receptor (VDR) on the ocular surface of patients with dry eye disease (DED) and controls without ophthalmological pathologies.Methods:Patients with DED without previous treatment were studied and compared with healthy subjects. Ocular Surface Disease Index assessement and ocular surface cytology were performed in all patients. The immunohistochemical expression of VDR was evaluated using fully automated immunohistochemistry. The evaluation involved multiplying the percentage of nuclear-labeled cells (0-100) by their intensity (0-3), resulting in a score ranging from 0 to 300 (VDR H-score). Squamous metaplasia was morphologically evaluated using liquid-phase cytology with Papanicolaou/periodic acid-Schiff staining using Nelson's grade (scale 0-3, higher grade to higher metaplastic change).Results:Eighteen patients with DED without previous treatment and 10 healthy subjects were studied. Squamous metaplasia was observed in 74% of patients with DED, in contrast to 0% in the control group. In patients with DED, there was a lower expression of VDR than in the control group (VDR H-score: 11.2 +/- 13.9 vs. 80.9 +/- 56; P = 0.0001). Furthermore, an inverse correlation was observed between Nelson grade and VDR H-score (P = 0.0001, rs = -0.71). No correlation was observed between Ocular Surface Disease Index and VDR H-score.Conclusions:This is the first study to evaluate the VDR in patients with DED. These patients presented with a lower expression of VDR than healthy subjects. No correlation was found with more severe symptoms. Patients with DED also had a higher frequency of squamous metaplasia.
dc.description.funderPontificia Universidad Catolica de Chile, from "Concursode Residentes 2022"
dc.fuente.origenWOS
dc.identifier.doi10.1097/ICO.0000000000003555
dc.identifier.eissn1536-4798
dc.identifier.issn0277-3740
dc.identifier.urihttps://doi.org/10.1097/ICO.0000000000003555
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/89769
dc.identifier.wosidWOS:001349626400007
dc.issue.numero12
dc.language.isoen
dc.pagina.final1546
dc.pagina.inicio1542
dc.revistaCornea
dc.rightsacceso restringido
dc.subjectvitamin D
dc.subjectdry eye disease
dc.subjectconjunctiva
dc.subjectimmunohistochemistry
dc.subjectcitology
dc.titleLow Expression of Vitamin D Receptor in Patients With Dry Eye Disease
dc.typeartículo
dc.volumen43
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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