The gene for mitochondrial ribosomal protein S14 has been transferred to the nucleus in <i>Arabidopsis thaliana</i>

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dc.contributor.authorFigueroa, P.
dc.contributor.authorGómez, I.
dc.contributor.authorCarmona, R.
dc.contributor.authorHoluigue, L.
dc.contributor.authorAraya, A.
dc.contributor.authorJordana, X.
dc.date.accessioned2025-01-21T01:31:56Z
dc.date.available2025-01-21T01:31:56Z
dc.date.issued1999
dc.description.abstractThe transfer of genetic information from the mitochondrion to the nucleus is thought to be still underway in higher plants. The mitochondrial genome of Arabidopsis thaliana contains only one rps14 pseudogene. In this paper we show that the functional gene encoding mitochondrial ribosomal protein S14 has been translocated to the nucleus. This gene transfer is a recent evolutionary event, which occurred within Cruciferae, probably after the divergence of Arabidopsis and Brassica napus. A 5' extension of the rps14 reading frame encodes a presequence which, in vitro, targets the polypeptide to isolated mitochondria and is cleaved off during or after import. No intron was found at the junction of the targeting presequence with the mitochondrially derived sequence, which are directly connected. By contrast, a 90-bp intron, which is removed by splicing to give a mature poly(A)(+) mRNA of 0.9 kb, is located in the 3' non-coding region. To our knowledge, this is the first report of an intron in such a position in a functional transferred gene in higher plants, and suggests that exon shuffling may have been involved in the acquisition of elements necessary for expression in the nucleus. Putative roles of this intron in polyadenylation and enhancement of gene expression are discussed.
dc.fechaingreso.objetodigital2025-02-16
dc.fuente.origenWOS
dc.identifier.issn0026-8925
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97172
dc.identifier.urihttps://doi.org/10.1007/s004380051068
dc.identifier.wosidWOS:000082577300016
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final144
dc.pagina.inicio139
dc.revistaMolecular and general genetics
dc.rightsacceso restringido
dc.subjectevolutionary gene transfer
dc.subjectplant mitochondria
dc.subjectribosomal protein S14
dc.subjectmitochondrial targeting peptide
dc.subjectnuclear intron
dc.subject.ods13 Climate Action
dc.subject.odspa13 Acción por el clima
dc.titleThe gene for mitochondrial ribosomal protein S14 has been transferred to the nucleus in <i>Arabidopsis thaliana</i>
dc.typeartículo
dc.volumen262
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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