Epigallocatechin Gallate Enhances MAL-PDT Cytotoxic Effect on PDT-Resistant Skin Cancer Squamous Cells

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dc.contributor.authorLeon, Daniela
dc.contributor.authorBuchegger, Kurt
dc.contributor.authorSilva, Ramon
dc.contributor.authorRiquelme, Ismael
dc.contributor.authorViscarra, Tamara
dc.contributor.authorMora-Lagos, Barbara
dc.contributor.authorZanella, Louise
dc.contributor.authorSchafer, Fabiola
dc.contributor.authorKurachi, Cristina
dc.contributor.authorRoa, Juan Carlos
dc.contributor.authorIli, Carmen
dc.contributor.authorBrebi, Priscilla
dc.date.accessioned2025-01-23T19:51:45Z
dc.date.available2025-01-23T19:51:45Z
dc.date.issued2020
dc.description.abstractPhotodynamic therapy (PDT) has been used to treat certain types of non-melanoma skin cancer with promising results. However, some skin lesions have not fully responded to this treatment, suggesting a potential PDT-resistant phenotype. Therefore, novel therapeutic alternatives must be identified that improve PDT in resistant skin cancer. In this study, we analyzed the cell viability, intracellular protoporphyrin IX (PpIX) content and subcellular localization, proliferation profile, cell death, reactive oxygen species (ROS) detection and relative gene expression in PDT-resistant HSC-1 cells. PDT-resistant HSC-1 cells show a low quantity of protoporphyrin IX and low levels of ROS, and thus a low rate of death cell. Furthermore, the resistant phenotype showed a downregulation of HSPB1, SLC15A2, FECH, SOD2 and an upregulation of HMBS and BIRC5 genes. On the other hand, epigallocatechin gallate catechin enhanced the MAL-PDT effect, increasing levels of protoporphyrin IX and ROS, and killing 100% of resistant cells. The resistant MAL-PDT model of skin cancer squamous cells (HSC-1) is a reliable and useful tool to understand PDT cytotoxicity and cellular response. These resistant cells were successfully sensitized with epigallocatechin gallate catechin. The in vitro epigallocatechin gallate catechin effect as an enhancer of MAL-PDT in resistant cells is promising in the treatment of difficult skin cancer lesions.
dc.fuente.origenWOS
dc.identifier.doi10.3390/ijms21093327
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttps://doi.org/10.3390/ijms21093327
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100598
dc.identifier.wosidWOS:000535581700298
dc.issue.numero9
dc.language.isoen
dc.revistaInternational journal of molecular sciences
dc.rightsacceso restringido
dc.subjectnon-melanoma skin cancer
dc.subjectphotodynamic therapy
dc.subjectsquamous cell carcinoma
dc.subjectmethyl aminolevulinate
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEpigallocatechin Gallate Enhances MAL-PDT Cytotoxic Effect on PDT-Resistant Skin Cancer Squamous Cells
dc.typeartículo
dc.volumen21
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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