Potential of mesenchymal stromal/stem cells and spermatogonial stem cells for survival and colonization in bull recipient testes after allogenic transplantation

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dc.contributor.authorSegunda, Moises N.
dc.contributor.authorCortez, Jahaira
dc.contributor.authorDiaz, Carlos
dc.contributor.authorArancibia, Richard
dc.contributor.authorTorres, Cristian G.
dc.contributor.authorParraguez, Victor H.
dc.contributor.authorde los Reyes, Monica
dc.contributor.authorPeralta, Oscar A.
dc.date.accessioned2025-01-20T16:07:17Z
dc.date.available2025-01-20T16:07:17Z
dc.date.issued2024
dc.description.abstractStem cell transplantation into seminiferous tubules of recipient testis could become a tool for fertility restoration, genetic improvement, or conservation of endangered species. Spermatogonial stem cells (SSCs) are primary candidates for transplantation; however, limited abundance, complexity for isolation and culture, and lack of specific markers have limited their use. Mesenchymal stromal/stem cells (MSCs) are multipotent progenitors that are simple to isolate and culture and possess specific markers for identification, and immune evasive and migratory capacities. The objective of the present study was to evaluate the potential for survival and colonization in seminiferous tubules of two different concentrations of bovine fetal adipose tissue-derived MSCs (ATMSCs), native of pre-induced, and to compare the fate of bovine adult peripheral blood-derived MSCs (PB-MSCs) and SSCs after allogenic transplantation in testis of recipient bulls. In experiment 1, AT-MSCs at two concentrations (1x107 and 2x107; n = 3) or pre-exposed to 2 mu M testosterone and 1 mu M retinoic acid (RA) for 14 days (n = 5) were evaluated. In experiment 2, adult PB-MSCs and SSCs (4x107 cells each) pre-exposed to Sertoli cell conditioned media (SCs/CM; n = 4) for 14 days were compared. Each cell type was separately labelled with PKH26 and then transplanted into testes of 8-month-old recipient bulls. Four weeks (Exp. 1) and two weeks (Exp. 2) after transplantation, testicular tissue was processed for confocal microscopy detection of PKH26-positive cells. Mean number of PKH26-positive cells were higher (P < 0.05) in testis transplanted with 2x107 AT-MSCs in the proximal (6.7 f 3.7) and medial (6.6 f 3.2) sections compared to testis transplanted with 1x107 ATMSCs (proximal: 1.9 f 1; medial: 1.9 f 1) sections or pre-induced AT-MSCs (proximal: 4.7 f 5.6; medial: 3.8 f 4.1). In Exp. 2, mean number of PKH26-positive SSCs in medial testicular section (22.5 f 1.3) were higher (P <0.05) compared to respective section in PB-MSCs group (17 f 4.2). Thus, in vivo data indicates that a higher number of transplanted AT-MSCs resulted in more cells surviving and colonizing seminiferous tubules; however, pre-induction with testosterone and RA did not improve these capacities. SSCs displayed a greater capacity for survival and colonization in recipient seminiferous tubules; however, PB-MSCs were observed in all sections of testis after two weeks of transplantation.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.theriogenology.2024.09.022
dc.identifier.eissn1879-3231
dc.identifier.issn0093-691X
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2024.09.022
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/89991
dc.identifier.wosidWOS:001327577800001
dc.language.isoen
dc.pagina.final202
dc.pagina.inicio192
dc.revistaTheriogenology
dc.rightsacceso restringido
dc.subjectBovine mesenchymal stromal/stem cell
dc.subjectSpermatogonial stem cells
dc.subjectStem cell transplantation
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePotential of mesenchymal stromal/stem cells and spermatogonial stem cells for survival and colonization in bull recipient testes after allogenic transplantation
dc.typeartículo
dc.volumen230
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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