Effect of CRISPR/Cas9 Targets Associated with Iron Metabolism and Its Variation on Transcriptional Regulation of SHK-1 Cell Line as a Model for Iron Metabolism

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dc.article.number198
dc.catalogadoraba
dc.contributor.authorDettleff Faundes, Phillip James
dc.contributor.authorYehwa Jin
dc.contributor.authorCarolina Peñaloza
dc.contributor.authorRodrigo Pulgar
dc.contributor.authorSáez Beltrán, Alejandro José
dc.contributor.authorDiego Robledo
dc.contributor.authorEscobar Aguirre, Sebastián Gonzalo
dc.date.accessioned2024-06-10T21:25:29Z
dc.date.available2024-06-10T21:25:29Z
dc.date.issued2024
dc.description.abstractIn this study, we investigated the function of a gene associated with iron metabolism using CRISPR-Cas9 and RNA sequencing in SHK-1 salmon cells. Our objective was to understand how different guide RNA (gRNA) sequences against the transferrin gene tf could influence gene expression and cellular processes related to iron uptake. RNA-Seq analysis was performed to evaluate the transcriptomic effects of two distinct gRNA targets with high knock-out (KO) efficiencies for the targeted tf gene in the SHK-1 genome. Our results showed no significant differential expression in transferrin-related transcripts between wild-type and CRISPR-edited cells; however, there were major differences between their transcriptomes, indicating complex transcriptional regulation changes. Enrichment analysis highlighted specific processes and molecular functions, including those related to the nucleus, cytoplasm, and protein binding. Notably, different sgRNAs targeting tf might result in different mutations at DNA levels in SHK-1 salmon cells.
dc.description.funderFONDECYT ANID
dc.description.funderBBSRC
dc.fechaingreso.objetodigital2024-06-10
dc.format.extent13 páginas
dc.fuente.origenORCID
dc.identifier.doi10.3390/fishes9060198
dc.identifier.eissn2410-3888
dc.identifier.urihttps://doi.org/10.3390/fishes9060198
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/86689
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Escobar Aguirre, Sebastian Gonzalo; 0000-0001-8826-2096; 1092624
dc.information.autorucEscuela de Medicina Veterinaria; Dettleff Faundes, Phillip James; S/I; 1252622
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Sáez Beltrán, Alejandro José; S/I; 1027712
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Escobar Aguirre, Sebastián Gonzalo; 0000-0001-8826-2096; 1092624
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final13
dc.pagina.inicio1
dc.revistaFishes
dc.rightsacceso abierto
dc.rights.licenseATTRIBUTION 4.0 INTERNATIONAL
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCRISPR-Cas9
dc.subjectIron metabolism
dc.subjectRNA-seq
dc.subjectGenome editing
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEffect of CRISPR/Cas9 Targets Associated with Iron Metabolism and Its Variation on Transcriptional Regulation of SHK-1 Cell Line as a Model for Iron Metabolism
dc.typeartículo
dc.volumen9
sipa.codpersvinculados1092624
sipa.codpersvinculados1252622
sipa.codpersvinculados1027712
sipa.trazabilidadORCID;2024-06-03
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